Staining Reagents
Sartorius Incucyte Reagent Kit
The MMP Orange Reagent Kit assesses changes in MMP an indicator of mitochondrial health. The cell-permeable MMP Orange Reagent accumulates in active mitochondria in proportion to the MMP generated by the electrochemical gradient across the mitochondrial membrane.
Enzo Life Sciences JC-1 (ultra pure) (5mg). CAS: 47729-63-5
JC-1 is widely used for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. However, upon cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to other commonly employed fluorescent probes of mitochondrial membrane potential is that it allows for both qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio. Wavelength Maxima: Excitation 515nm, Emission 529nm Alternative name: 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide. Purity: ≥95% (TLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Electron Microscopy Sciences Nigrosin 25 GR
(Acid Black 2; Gray R, B, BB; Indulin Black; Nigrosin W, WL ; Silver Gray; Steel Gray)
Solubility: 10.0% Water; 0.0% Alcohol; 1.0% Cellosolve; 11.0% Glycol; 0.0% Xylene
Nigrosin is also known as Acid Black 2; Gray R, B, BB, Indulin Black, Nigrosin W, WL , Silver Gray and Steel Gray. It is used for negative staining of bacteria. With Basic Fuchsin, staining bacteria spores. Dorner, Le Lait, 6, 8 (1926). Biochem. Biophys. Acta 69,159 (1963).
Electron Microscopy Sciences Safranin O, Certified, C.I. DcS-25 25 GR
(Safranin T, Y or A; Basis Red 2; Cotton Red; Gossypimine)
C₂₀H₁₉N₄Cl F.W. 350.84
Solubility: Water; 3.5% Alcohol; 5.0% Cellosolve; 3.5% Glycol; 0.0% Xylene
Also known as Safranin T, Y or A, Basis Red 2, Cotton Red and Gossypimine.
Electron Microscopy Sciences Papanicolaou Stain Solution, OG-6 1 LT
Papanicolaou Stain Solution, OG-6 solution is a prepared, ready-to-use, high quality staining solution that saves time and effort and produces consistent uniform results.
IMEB, Inc Masson Trichrome Stain Kit, Includes: 1- 8 Oz Bottle/Solutions:Bouin’s, Biecrich’s Scarlet/Acid Fuchsin, Phosphotungstic/Phosphomolybdic Acid, Hematoxylin, 4/2oz Staining Containers, & Storage Tray
Masson Trichrome stain used for collagen, muscle and pituitary cells. Includes: 1- 8 oz. Bottle Bouin’s solution, 1- 8 oz. Bottle Biecrich’s Scarlet/acid Fuchsin solution, 1- 8 oz. Bottle Phosphotungstic/ Phosphomolybdic acid solution, 1- 8 oz. Bottle Hematoxylin solution, 4- 2 oz. Staining containers and storage tray
Electron Microscopy Sciences Aurion Cold Water Fish Skin Gelatine 40% 10 ML
The use of Cold Water Fish Skin Gelatin to prevent background reactions has been recommended by e.g. Behnke et al. (J. Cell Biol. 41, [1986], 386). The product is supplied as a liquid concentrate (40%).
Cayman Chemical Malachite Green Phosphate Ass
Cayman’s Malachite Green Phosphate Assay provides a fast, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on the formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm. Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates. This assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate and is amenable to high-throughput screening applications. The assay is formatted to a 96-well format, but could EAsily be modified for use in 384-well or cuvette-based assays.
Aqua Solutions Phenolphthalein 1% w/v in Isopropanol | 77-09-8 | MFCD00005913 | 250ml
Phenolphthalein 1% w/v in Isopropanol | MW: 318.327 | 77-09-8 | MFCD00005913 | 250ml
Electron Microscopy Sciences Modified Ea Staining Solution 500 ML
Used in Papanicolaou Stain for Membrane Filter. Reference: Gill, G.W., ActaCytol., 18:300-311, 1974, Diagnostic Cytology by Membrane Filter, Application Bulletin 100, Gelman Instrument Co., Ann Arbor, Michigan, 1978
Chem-Impex International, Inc. Methylene Blue Trihydrate | 7220-79-3 | MFCD00150008 | 100G
Methylene Blue Trihydrate, 7220-79-3, MFCD00150008, 100G
Chem-Impex International, Inc. Metalphthalein | 2411-89-4 | MFCD00005911 | 25G
Metalphthalein, 2411-89-4, MFCD00005911, 25G
GFS Chemicals Inc VITEX, (Modified Starch) | 100 G | GFS CHEMICALS, INC. Item #: 76601 | Bulk #: 331
VITEX, (Modified Starch) | Chemical Formula : | CAS # : 9005-84-9 | Specifications : Insoluble 0.01 % , Suitablilty as iodometric indicator
Electron Microscopy Sciences Weigerts Iron Hematoxylin, Solution B 500 ML
For use in Truant's Fluorescent Method for Acid Fast - Organism For bacterial stain. See Truant, J.P. Henry Ford Hospital Med. Bull., 10:287, 1962. AFIP Manual of Histological Staining Methods, 3rd ed. Ed. L. Luna: New York: McGraw Hill Publications. c. 1968. p. 219. or Clark, G.: Staining Procedures. Williams and Wilkens Co., Baltimore. 3rd edition. c.1973. p. 225