Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 5kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
Bioworld Tricine-SDS Sample Buffer, Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, 0.01% phenol red, and 4% β-mercaptoethanol.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Kd Medical Inc TAE (TRIS-ACETATE EDTA), 50X
TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. 50X concentrated ready to be diluted to a 1X working solution. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 8 Bis-Tris Precast
mPAGE 8% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Crescent Chemical Co Inc TBE BUFFER (10X)
TBE Buffer (10x) 10 x concentrated aqueous solution.TBE Buffer is widely used for the electrophoresis of nucleic acids and has a higher buffer capacity than TAE. It can be used for DNA and RNA polyacrylamide and agarose gel electrophoresis. Size - 2x500ML
Biotang Inc TRIS-HEPES-SDS Running Buffer (10X), 500 ml
TRIS-HEPES-SDS Running Buffer (10X), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml.
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
Biotang Inc BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml
BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml.