Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ Tris Buffer, 0.5M, 10X, pH 7.6, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Teknova DNA Suspension Buffer, pH 8.0, 10L, DNase/RNase Tested, PCR Grade, Sterile. 2 Pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
![eMolecules tert-butyl 8-(hydroxymethyl)-2-azaspiro[4.5]decane-2-carboxylate | 1420955-72-1 | MFCD24384294 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159466.JPG-250.jpg)
eMolecules tert-butyl 8-(hydroxymethyl)-2-azaspiro[4.5]decane-2-carboxylate | 1420955-72-1 | MFCD24384294 | 1g
Pharmablock | tert-butyl 8-(hydroxymethyl)-2-azaspiro[4.5]decane-2-carboxylate | 1g | 551108067 | PBJL100461 | | 1420955-72-1 | MFCD24384294 | 269.385 | C15H27NO3
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Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Quality Biological Inc TAE Buffer 10X, 1000 mL
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE is often prepared in concentrated stock solutions of 10X or 50X in the laboratory. ¿ A 1X working solution is prepared prior to electrophoresis. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 41105323 ¿ ¿ Max Shelf Life: 36 months
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(tert-butyl) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 50 MG
NODAGA-tris(t-Bu ester) is a tert-butyl-protected bifunctional chelator precursor used for covalent attachment to biomolecules. It is designed to conjugate to peptides, proteins, or antibodies; after deprotection the chelator can be radiolabeled for PET imaging studies. The reagent is supplied as a high-purity research chemical suitable for labeling workflows and analytical characterization.
- Tert-butyl protected chelator precursor suitable for conjugation to peptides and antibodies.
- Compatible with deprotection and subsequent radiolabeling for PET imaging.
- High purity as measured by HPLC, appropriate for research use.
- Soluble in DMSO for formulation and conjugation workflows.
- Molecular weight 543.69 g/mol; chemical formula C27H49N3O8.
Teknova 5X TBE DNA Sequencing Grade Solution. 4L, Non-Sterile. 4 pack.
445mM Tris, 445mM Boric Acid, 10mM Ethylenediaminetetraacetic acid (EDTA)
Kd Medical Inc TRIS-HCL BUFFER 1M, PH 7.5, 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 7.5. One 1 liter bottle.
Abcam Antigen Retrieval Buffer (100 x Tris-EDTA Buffer, pH 9.0), 125ML
Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification
The product is subject to the following: Abcam Restricted Use Statement
Biotang Inc Nylon Membrane stripping Buffer(10X), 250 ml
Nylon Membrane stripping Buffer(10X), 250 ml.
eMolecules tert-butyl 3-(hydroxymethyl)-3-methylpiperidine-1-carboxylate | 406212-48-4 | MFCD21642046 | 1g
Pharmablock | tert-butyl 3-(hydroxymethyl)-3-methylpiperidine-1-carboxylate | 1g | 551129390 | PBN2011457 | | 406212-48-4 | MFCD21642046 | 229.320 | C12H23NO3
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Electron Microscopy Sciences Tris HCL Buffer, pH range 7.2-9.0 250 ML
Please specify pH when ordering.
pH: 7.2-9.0