Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova SM Buffer. 500mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
LAB BIOREAGENTS LowBinding III Buffer 250ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Alkali Scientific CellPro™ TBE 10X (Tris-Borate EDTA), 1L
Alkali Scientific™ CellPro™ TBE (Tris-borate EDTA) solution offers a sterile and filtered formulation of this essential buffer for molecular biology and electrophoresis applications. With its meticulous preparation, this solution ensures purity and consistency, meeting the rigorous demands of scientific research and experimentation. The sterile nature of the solution minimizes the risk of contamination, making it ideal for various molecular biology techniques, including DNA and RNA agarose gel electrophoresis. Additionally, the filtered formulation guarantees clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used for nucleic acid separation, DNA fragment analysis, or other molecular biology procedures, CellPro™ TBE solution delivers consistent performance and reproducible results, supporting the success of laboratory workflows.
Bioworld Tris-Glycine Sample Buffer (4X), 25 mL
Product DescriptionTris-Glycine (TG) sample buffer has a concentration of 4X. It contains 400 mM Tris-HCl at a pH of 8.6, 40% glycerol, and 0.02% bromophenol blue.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Bioworld TE (Tris-EDTA) Buffer, Sterile, 100 mL
Product DescriptionTE (Tris-EDTA) is a sterile buffer that has a pH level of 8.0 brought up with HCl and is stored at room temperature. It contains 10 mM Tris and 1 mM EDTA. It is of biotechnology grade and no DNAse activity is detected.TE buffer is used to solubilize DNA or RNA while protecting it from degradation. Commonly used for DNA isolation and electrophoresis protocols.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4) - 4x1L
Tris Buffered Saline-Tween TBST (10X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 100 g
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration), Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol.
Crescent Chemical Co Inc EDTA ANALYTICAL GRADE
Ethylenediamine tetraacetic acid analytical grade Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 11278.02 CAS 60-00-4
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 100G GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade, multicompendial
S2 Media 1M Tris-HCl, pH 8.0, Sterile, 1L Media Bottle, 1ea
1M Tris-HCl Buffer (sterile) is commonly used in labs for pH stabilization in biochemical reactions, as a buffer in analytical chemistry, and as a sample diluent near neutral to slightly basic pH. At pH 8.0, Tris (pKa ≈ 8.1 at 25°C) provides effective buffering between pH 7–9, making it compatible with most biological systems and minimally reactive with biomolecules.• 1M Tris-HCl should be used by trained professionals within a laboratory setting. • Tris is an abbreviation for tris(hydroxymethyl)aminomethane).• Product is not intended for use in the diagnosis or treatment of disease or other human conditions.• For Research Use Only (RUO). Not for use in diagnostic procedures.
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 1 kg
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration), Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol. Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.
Kd Medical Inc TRIS HCL, 1M, PH 7.5, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.5. One 500ml bottle.