Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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2,251 – 2,265 of 3,503 results

2,251

Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.

MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.

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2,252

Biotang Inc HEPES-Buffer 0.5M pH, 8.5, 100 ml

HEPES-Buffer 0.5M pH, 8.5, 100 ml.

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2,253

ILEX LIFE SCIENCES LLC PAN-Biotech HEPES Buffer 1M (100 ml)

HEPES Buffer 1M. QUANTITY: 100 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. COMPOSITION: HEPES 238.31 g/L.

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2,254

Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 100 mL

Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0

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2,255

Biotang Inc MOPS Buffered Saline 5X, pH 7.0. 125 mM MOPS, 750 mM Sodium Chloride, pH 7.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-570.

MOPS Buffered Saline 5X, pH 7.0. 125 mM MOPS, 750 mM Sodium Chloride, pH 7.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-570.

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2,256

Alkali Scientific MOPSO Sodium Salt, 2 Kg

MOPSO Sodium Salt is a buffering reagent used to stabilize pH in laboratory applications. The 2 kg weight offers ample supply for large-scale experiments, ensuring consistency and reproducibility in analytical procedures. This sodium salt of MOPSO (Morpholinopropanesulfonic acid) provides excellent buffering capacity, especially in the pH range of 7.0–8.5, which is critical for maintaining the integrity of sensitive biological samples and chemical reactions. MOPSO is frequently used in the preparation of buffers for chromatography, electrophoresis, and enzyme assays. Its ability to maintain pH stability in various solutions makes it an essential reagent for biochemical and pharmaceutical applications.

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2,257

Medchemexpress LLC MES hydrate | 1266615-59-1 | MFCD00149409 | 99.9% | 10 G

MES (2-Morpholinoethanesulphonic acid) hydrate is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15. Its pKa value changes with temperature, a factor to consider when used at varying temperatures. MES hydrate exhibits good buffering properties in biological experiments and has a poor affinity for metal ions, making it suitable for studies involving such ions.

Amphoteric ion buffer
Buffer capacity ranging pH 5.5-7.0
Widely used in biochemistry and molecular biology experiments
Effective buffering capacity at approximately pH 6.15
Poor affinity with metal ions

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2,258

Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES

NC4005335 HEPES EMPROVE EVOLVE 100G

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2,259

Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg

PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a biochemical buffer available in USP-grade, designed for a wide range of scientific and research applications. This 5kg container is ideal for laboratories that require large volumes of buffer solution for high-throughput processes, including protein studies, enzymatic assays, and cell culture applications. PIPES is known for its ability to maintain a stable pH under various conditions where other buffers may not be suitable. It is especially effective in high-salt and high-temperature environments. The 5kg pack ensures that large-scale laboratory projects can proceed without the need for frequent reordering, making it both a cost-effective and convenient solution.