Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659846.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 100 G
PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt] is a high-quality laboratory reagent supplied in a 100g bottle. This sodium salt form of PIPES is used to prepare buffers with precise pH control, particularly in biological and biochemical research. The sodium salt version is more soluble in water than the free acid form, making it ideal for high-concentration buffer solutions. It is widely used in protein purification, enzymatic assays, and molecular biology applications that require pH stability under varying conditions. The 100g pack size is suitable for small-scale experiments, and the reagent provides a reliable means of controlling pH in critical laboratory workflows.
Alkali Scientific MES, Anhydrous, 25 G
MES, Anhydrous, 25 G, is a small-volume, high-purity buffering agent used to maintain pH stability in laboratory experiments. With its anhydrous form, it dissolves readily in solvents for precise pH adjustments. MES buffers in the pH range of 5.5 to 6.7, making it particularly useful in applications such as electrophoresis, enzyme assays, and in preparing cell culture media. The 25 g package is ideal for small-scale experiments or for laboratories that require frequent pH adjustments but do not need larger quantities of the reagent. MES is an essential tool for many biochemical and biological processes that require exacting conditions for reproducible results.
AG Scientific Inc MOPS Free Acid 1 KG
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions. The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
Medchemexpress LLC MOPS sodium salt | 71119-22-7 | 99.8% | 500 G
MOPS sodium salt is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It significantly improves the thermal stability of BSA. MOPS sodium salt is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.
- Purity: 99.8%
- Appearance: Solid
- Color: White to off-white
- Storage: 4°C, sealed, away from moisture. In solvent: -80°C for 6 months; -20°C for 1 month (sealed, away from moisture).
- Solubility in H2O: 100 mg/mL (needs ultrasonic)
- Solubility in DMSO: 50 mg/mL (needs ultrasonic; use newly opened DMSO)
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 49 Grams of Powder, Makes 10 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (10 liters, 49 grams of powder) is designed for plant tissue culture applications that require large volumes of prepared solution. This medium is enriched with essential nutrients, growth regulators, and MES buffer, which helps to stabilize the pH during plant tissue growth. The formula ensures optimal growth conditions for plant cells, tissues, and organs in vitro, making it ideal for shoot multiplication, regeneration, and propagation experiments. It is commonly used in commercial plant propagation, agricultural research, and plant breeding. The large 10-liter yield makes this medium suitable for labs and research facilities with higher throughput needs.
eMolecules 58884-35-8 | 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | 58884-35-8 | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659807.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Free Acid is a biochemical reagent used for maintaining a stable pH environment in biological and chemical applications. This 250g package is ideal for preparing buffered solutions for use in cell culture, protein analysis, and other biochemical experiments. HEPES, or 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, is widely used because of its excellent buffering capacity in the pH range of 6.8 to 8.2. It is commonly used in cell biology to maintain physiological pH during experiments. This reagent is highly stable, non-toxic to cells, and is ideal for applications that require precise pH control over extended periods of time.
Biotang Inc HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080
HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
ILEX LIFE SCIENCES LLC PAN Biotech HEPES Buffer 1 M GMP Grade Cell Culture Buffer 100 ML 1/EA
PAN-Biotech HEPES Buffer 1 M GMP Grade 100 ml INTRODUCTION HEPES is a zwitterionic sulfonic acid buffering agent widely used in cell culture largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers GMP GRADE PAN-Biotech HEPES Buffer 1 M GMP Grade is a ready-to-use highly concentrated buffer solution designed for direct use in GMP-regulated clinical and industrial applications The GMP grade version is based on the proven HEPES formulation and is manufactured under strictly controlled GMP conditions Defined raw materials high batch-to-batch consistency and comprehensive documentation make HEPES Buffer 1 M GMP a true ready-to-use solution suitable for immediate use in sensitive cell culture and manufacturing workflows COMPOSITION HEPES 238 31 g/L QUALITY GMP Grade (EU Annex 1) LIQUID/POWDER Liquid STERILE Yes STORAGE 2-8 C