Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.8 (0.2 micron filtered), 500 ml.
eMolecules 2044704-80-3 | tert-butyl 2-(hydroxymethyl)-4-methyl-1H-indole-1-carboxylate | MFCD30489087 | 1g
Ambeed | 2-Bromo-4-(bromomethyl)-1-(trifluoromethyl)benzene | 250mg | 600846886 | A744018 | 372120-77-9 | MFCD13185413 | 317.931 | C8H5Br2F3
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool
Rockland Immunochemicals TM Buffer (50 mM Tris Cl, pH 7.5, 10 mM Magnesium Sulfate) - MB-059
1X TM Buffer (50 mM Tris HCl, 10 mM Magnesium Sulfate at pH 7.5) is suitable for molecular biology assays.
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 5kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
Kd Medical Inc TAE 10X 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 10X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer Bi
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This SOLUTIONon is effective under slightly basic conditions which keeps DNA deprotonated water-soluble and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
eMolecules 1065608-51-6 | TERT-BUTYL 4-(HYDROXYMETHYL)AZEPANE-1-CARBOXYLATE | MFCD18088895 | 1g
ChemScene | 2-Bromo-34-difluoro-6-nitroaniline | 100mg | 714104035 | CS-0146683 | 112062-62-1 | MFCD20441752 | 253.003 | C6H3BrF2N2O2
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool
Research Products International Corp TG 10X Solution, 2 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 4 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Teknova DNA Suspension Buffer, pH 8.0, 10L, DNase/RNase Tested, PCR Grade, Sterile. 2 Pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Sigma Aldrich Fine Chemicals Biosciences Tris-Glycine Buffer 10x Co1L
TRIS Tris-Glycine Buffer 10x Co1L
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 100g
Trizma base | Purity: 99.9% | Mol Wt: 121.41 | 77-86-1 | MFCD00004679 | 100g