Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hampton Research Tris Buffer
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL) Please note items from this supplier are non-returnable.
Alkali Scientific Tris Acetate Ultra Pure, 500 G
Tris Acetate Ultra Pure is a high-quality buffering agent formulated for high-precision laboratory applications. With its ultra-pure grade, it ensures minimal interference in sensitive experiments, maintaining a stable pH environment. This 500g bottle is ideal for medium-scale preparations, supporting a wide range of biochemical and molecular biology applications, including electrophoresis, protein assays, and enzyme reactions. The tris acetate buffer is particularly effective for experiments requiring consistent pH conditions, making it a reliable choice for researchers working in protein analysis, DNA/RNA extraction, and various other scientific fields where precision is critical.
Bioworld Tricine-SDS Sample Buffer, Non-Reducing (2X), 25 mL
Product DescriptionTricine-SDS is a sample buffer under non-reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, and 0.01% phenol red.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Alkali Scientific TRIS Buffered Saline, 10X Solution, 1 L
TRIS Buffered Saline (TBS) 10X solution is a concentrated buffer used in various biological applications, including immunohistochemistry, protein assays, and cell biology research. This 1L bottle of 10X solution is designed for dilution to 1X working concentration, making it convenient for a wide range of protocols. TBS provides a stable, isotonic environment that is ideal for washing cells, diluting samples, and preparing buffers for antibody conjugation. The buffering capacity of TRIS ensures stable pH conditions, making TBS essential for maintaining protein and enzyme functionality during experiments. It is commonly used for western blotting, enzyme-linked immunosorbent assays (ELISA), and other immunological procedures.
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959188 ELUTE BUFFER B 50MM TRIS HCL8
![Alkali Scientific TG 10X Solution [TRIS Glycine Buffer 10X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661625.jpg-250.jpg)
Alkali Scientific TG 10X Solution [TRIS Glycine Buffer 10X Solution], 1 Liter
TRIS Glycine Buffer 10X Solution is a high-performance buffer designed for use in electrophoresis, particularly for running SDS-PAGE gels. The 1-liter bottle of 10X solution allows for efficient dilution and preparation of multiple electrophoresis runs. This buffer system is well-suited for separating a wide range of proteins, providing reliable and consistent results. The TRIS-Glycine formulation is known for its excellent buffering capacity and is widely used in molecular biology and biochemistry for protein analysis. This buffer is compatible with numerous protein electrophoresis protocols, including those for high-precision applications such as Western blotting and protein quantification.
Nusep Inc NUVIEW UNIV GEL 10 10-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 10% 10well. Cassette size: 10 x 8 cm. Fits Hoefer, Cytiva, Merck Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.