Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Buffer; National Diagnostics; Tris-Glycine-SDS PAGE (10X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Size: 1L
National Diagnostics PROTOGEL RESOLVING BUFFER 1 LT
ProtoGel Resolving Buffer (4X), 4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE, 1 LT
Hoefer, Inc MSML PRECSTGEL 10 BIS-TRIS 12W
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.#11;Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cm#11;Wells: 12-well, 15-well Concentration:#11;• Gradient gels: 4-12%ĉĉĉĉĉĉĉĉĉĉMighty Small precast gels, 10% Bis-Tris, 12wellsĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
Boston Bioproducts Inc MES-SDS Buffer (20X) - 1L
The 20X MES-SDS Running Buffer is formulated specifically for running proteins on Bis-Tris gels for separating small to medium-sized proteins under denaturing conditions in SDS-PAGE. When reconstituted from the 20X concentrate, this buffer maintains a stable pH of approximately 7.3. The MES-SDS Running Buffer enables faster and efficient resolution of well-defined protein bands in comparison to other running buffers in SDS-PAGE experiments.
Phoenix Flow Systems Inc CHEAP SHEATH PBS CONCENTRATE
Cheap Sheath Is A Concentrated Ph Balanced Formulation Of Phosphate Buffered Saline Which Has Been Pre-Filtered And Is Ready To Use In Any Flow Cytometer As Your Sheath Solution Upon Dilution With Distilled Water. Each Bottle Of Cheap Sheath Contains Enough Concentrate To Prepare 20 Liters Of Flow Cytometer Sheath Soluton. Formulation Is Simple For Flow Cytometers That Do Not Use 20 Liter Sheath Containers. For Any Flow Cytometer With A 3.5 Liter Sheath Tank, Add 100 Mls Cheap Sheath To 2.9 Liters Of Distilled Water In The Sheath Tank. 1Qt Bottle (2 Lbs).
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G
Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
Mitegen Bis-Tris/ HCl, 1M, pH5.2, 250 ml, CAS # 6976-37-0
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Sigma Aldrich Fine Chemicals Biosciences HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 500g
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | Purity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 500g
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, for Western Blot Washing) - 1L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration, and is useful for washing in Western Blotting.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 6.8) - 100ml
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.