Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
BLD PHARMATECH CO LIMITED Tris(perfluorophenyl)borane, 1109-15-5, Purity: 97%, 10g
Tris(perfluorophenyl)borane, 1109-15-5, Purity: 97%, 10g
eMolecules tert-butyl 3-(hydroxymethyl)-4-methyl-pyrrolidine-1-carboxylate | 1184985-22-5 | MFCD08461237 | 1g
Pharmablock | tert-butyl 3-(hydroxymethyl)-4-methyl-pyrrolidine-1-carboxylate | 1g | 551098866 | PB96772 | | 1184985-22-5 | MFCD08461237 | 215.293 | C11H21NO3
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Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 10G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 10G
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 500G
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 500G
Medchemexpress LLC N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid | 10191-18-1 | 98.0% | 500 G
BES is a secondary standard biochemical buffer used in diagnostic testing. It offers a stable pH environment for various applications.
- Effective pH range of 6.4 to 7.8
- pKa of 7.15 at 20 °C
- Suitable for diagnostic testing areas
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS Propane | 64431-96-5 | MFCD00004689 | 100g
BIS-TRIS Propane | Purity: 99% | Mol Wt: 282.33 | 64431-96-5 | MFCD00004689 | 100g
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959194 10X ADJ BUF 1.0M NACL .5M THCL
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959187 ELUTE BUFFER A 50MM TRIS HCL8
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959188 ELUTE BUFFER B 50MM TRIS HCL8
Quality Biological Inc PBS (10X) pH 7.2 20L
PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications such as washing cells before dissociation transporting cells or tissue diluting cells for counting and preparing reagents.Endotoxin and Performance tested Manufactured in an ISO 90012015 certified facility.
