Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Abcam 10X ANNEXIN V/ANXA5 BINDING BU ..
10X ANNEXIN V/ANXA5 BINDING BU ..
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences HANKS BALANCED SALT SOLUTION
NC3660344 HANKS BALANCED SALT SOLUTION
Bio Rad Laboratories XT MES RUNNING BUFFER 500ML
XT MES Running Buffer, 20x, 500mL, Liquid, White, Odorless, Boiling Point: 100degreeC, pH: 6.4 at 20degreeC, Fully miscible in water, No special requirements for storage
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 4L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Boston Bioproducts Inc MES Buffer (1 M, pH 6.0) - 500ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Abcam 10 x RIPA Buffer, 15ML
10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins
The product is subject to the following: Abcam Restricted Use Statement
Fd Neurotechnologies Inc PHOSPHATE-BUFFERED 4% PARAFORM
This 1000ml filtered and ready-to-use tissue fixative is freshly made from high purity paraformaldehyde powder. This solution may be stored at room temperature for at least one year without change of the original quality. The standard solution contains 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Other concentrations can also be provided upon request.
Beckman Coulter Flow-Check Pro Fluorospheres
Flow-Check Pro Fluorospheres is a suspension of fluorescent microspheres used for daily verification of the Navios™or FC 500 Flow Cytometer optical alignment and fluidics system.
Lonza Walkersville Tris Buffer 50 mM
Tris buffer can be used in place of water as the sample diluent for highly acidic or basic samples (for endotoxin testing, samples and sample dilutions should be between pH 6-8). Tris buffer may be used to prepare samples for any of our endotoxin detection assays. Contains <0.005 EU/ml endotoxin.Storage: 2°C - 8°CContent:1 vial