Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

Search Within Results

421 – 435 of 3,501 results

421

Thermo Scientific Chemicals BIS-TRIS propane, 0.2M buffer soln., pH 6.5

CAS: 64431-96-5 Molecular Formula: C11H26N2O6 Molecular Weight (g/mol): 282.337 MDL Number: MFCD00004689 InChI Key: HHKZCCWKTZRCCL-UHFFFAOYSA-N PubChem CID: 125132 ChEBI: CHEBI:40947 IUPAC Name: 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CNC(CO)(CO)CO)CNC(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	125132
CAS	64431-96-5
Molecular Weight (g/mol)	282.337
ChEBI	CHEBI:40947
MDL Number	MFCD00004689
SMILES	C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
IUPAC Name	2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol
InChI Key	HHKZCCWKTZRCCL-UHFFFAOYSA-N
Molecular Formula	C11H26N2O6

422

HEPPS, (Crystalline),MP Biomedicals

CAS: 16052-06-5 Molecular Formula: C9H20N2O4S Molecular Weight (g/mol): 252.329 InChI Key: OWXMKDGYPWMGEB-UHFFFAOYSA-N Synonym: hepps,epps,4-2-hydroxyethyl-1-piperazinepropanesulfonic acid,3-4-2-hydroxyethyl piperazin-1-yl propane-1-sulfonic acid,1-piperazinepropanesulfonic acid, 4-2-hydroxyethyl,n-2-hydroxyethyl piperazine-n'-3-propanesulfonic acid,4-2-hydroxyethyl piperazine-1-propanesulfonic acid,4-2-hydroxyethyl-1-piperazinepropane sulfonic acid,4-2-hydroxyethyl-1-piperazinepropanesulphonic acid PubChem CID: 85255 ChEBI: CHEBI:42298 IUPAC Name: 3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid SMILES: C1CN(CCN1CCCS(=O)(=O)O)CCO

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Specifications

PubChem CID	85255
CAS	16052-06-5
Molecular Weight (g/mol)	252.329
ChEBI	CHEBI:42298
SMILES	C1CN(CCN1CCCS(=O)(=O)O)CCO
Synonym	hepps,epps,4-2-hydroxyethyl-1-piperazinepropanesulfonic acid,3-4-2-hydroxyethyl piperazin-1-yl propane-1-sulfonic acid,1-piperazinepropanesulfonic acid, 4-2-hydroxyethyl,n-2-hydroxyethyl piperazine-n'-3-propanesulfonic acid,4-2-hydroxyethyl piperazine-1-propanesulfonic acid,4-2-hydroxyethyl-1-piperazinepropane sulfonic acid,4-2-hydroxyethyl-1-piperazinepropanesulphonic acid
IUPAC Name	3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid
InChI Key	OWXMKDGYPWMGEB-UHFFFAOYSA-N
Molecular Formula	C9H20N2O4S

423

MilliporeSigma™ Tris Base, ULTROL™ Grade, Calbiochem™,

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: tris(Hydroxymethyl)aminomethane PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	tris(Hydroxymethyl)aminomethane
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

424

MilliporeSigma™ Tricine, ULTROL™ Grade, Calbiochem™,

CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-[tris(Hydroxymethyl)methyl]glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	79784
CAS	4-1-5704
Molecular Weight (g/mol)	179.17
ChEBI	CHEBI:39063
SMILES	C(C(=O)O)NC(CO)(CO)CO
Synonym	N-[tris(Hydroxymethyl)methyl]glycine
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula	C6H13NO5

425

MilliporeSigma™ MES, ULTROL™ Grade, Calbiochem™,

CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic Acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O

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Specifications

PubChem CID	78165
CAS	4432-31-9
Molecular Weight (g/mol)	195.233
ChEBI	CHEBI:39005
SMILES	C1COCCN1CCS(=O)(=O)O
Synonym	2-(N-Morpholino)ethanesulfonic Acid
IUPAC Name	2-morpholin-4-ylethanesulfonic acid
InChI Key	SXGZJKUKBWWHRA-UHFFFAOYSA-N
Molecular Formula	C6H13NO4S

426

HEPES Sodium Salt, Biological Buffer, High Purity, 98%, Spectrum™ Chemical

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

Pricing & Availability
Specifications

CAS	75277-39-3
Molecular Weight (g/mol)	260.28
SMILES	[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
IUPAC Name	sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO4S

427

Gibco™ HBSS, calcium, magnesium

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

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428

Tricine, Electrophoresis Grade, MP Biomedicals

CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	79784
CAS	4-1-5704
Molecular Weight (g/mol)	179.17
ChEBI	CHEBI:39063
SMILES	C(C(=O)O)NC(CO)(CO)CO
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula	C6H13NO5

429

Bis-Tris, ≥99%, MP Biomedicals™

CAS: 6976-37-0 Molecular Formula: C8H19NO5 Molecular Weight (g/mol): 209.242 MDL Number: MFCD00002853 InChI Key: OWMVSZAMULFTJU-UHFFFAOYSA-N Synonym: 2,2-bis(Hydroxymethyl)-2,2',2''-nitrilotriethanol,2, 2-bis(Hydroxymethyl)-2, 2' PubChem CID: 81462 ChEBI: CHEBI:41250 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CO)N(CCO)C(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	81462
CAS	6976-37-0
Molecular Weight (g/mol)	209.242
ChEBI	CHEBI:41250
MDL Number	MFCD00002853
SMILES	C(CO)N(CCO)C(CO)(CO)CO
Synonym	2,2-bis(Hydroxymethyl)-2,2',2''-nitrilotriethanol,2, 2-bis(Hydroxymethyl)-2, 2'
IUPAC Name	2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
InChI Key	OWMVSZAMULFTJU-UHFFFAOYSA-N
Molecular Formula	C8H19NO5

430

POPSO, 0.2M buffer soln., pH 8.0, Thermo Scientific™

CAS: 108321-07-9 Molecular Formula: C10H20N2Na2O8S2 Molecular Weight (g/mol): 406.376 MDL Number: MFCD00070005 InChI Key: XMRCLLXGMGEVHS-UHFFFAOYSA-L PubChem CID: 53393457 IUPAC Name: disodium;2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazin-1-yl]propane-1-sulfonate SMILES: C1CN(CCN1CC(CS(=O)(=O)[O-])O)CC(CS(=O)(=O)[O-])O.[Na+].[Na+]

Pricing & Availability
Specifications

PubChem CID	53393457
CAS	108321-07-9
Molecular Weight (g/mol)	406.376
MDL Number	MFCD00070005
SMILES	C1CN(CCN1CC(CS(=O)(=O)[O-])O)CC(CS(=O)(=O)[O-])O.[Na+].[Na+]
IUPAC Name	disodium;2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazin-1-yl]propane-1-sulfonate
InChI Key	XMRCLLXGMGEVHS-UHFFFAOYSA-L
Molecular Formula	C10H20N2Na2O8S2

431

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 6.8

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Pricing & Availability
Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

432

MP Biomedicals™ SM Buffer

SM Buffer is a sterile solution.

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433

Gibco™ PBS Tablets

Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

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434

Gibco™ Tris Base Solutions

Gibco concentrates are intended for dilution at the point of use and come sterile-filtered and packaged in conveniently sized bioprocess containers. Sourcing concentrated stock solutions can help streamline buffer preparation by helping reduce preparation time, storage space, and the manufacturing footprint required for hydration.

Pricing & Availability
Specifications

Percent Purity	Not Available
CAS	77-86-1
For Use With (Equipment)	ILC/ILD System
Molecular Weight (g/mol)	Raw Material: 121.14
Packaging	Bioprocess Container in Secondary Packaging
Physical Form	Solution
pH	10.0 to 12.0
Manufacturing Quality	CGMP
Grade	EP/USP
For Use With (Application)	Bioproduction
Solution Type	Buffer