Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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421 – 435 of 3,556 results

421

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PIPES, Monosodium Salt, Biological Buffer, High Purity, 98.5%, Spectrum™ Chemical

CAS: 10010-67-0 Molecular Formula: C8H17N2NaO6S2 Molecular Weight (g/mol): 324.34 MDL Number: MFCD00065472 InChI Key: OGGAIRCLBMGXCZ-UHFFFAOYSA-M IUPAC Name: sodium 2-[4-(2-sulfoethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OS(=O)(=O)CCN1CCN(CCS([O-])(=O)=O)CC1

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Specifications

CAS	10010-67-0
Molecular Weight (g/mol)	324.34
MDL Number	MFCD00065472
SMILES	[Na+].OS(=O)(=O)CCN1CCN(CCS([O-])(=O)=O)CC1
IUPAC Name	sodium 2-[4-(2-sulfoethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	OGGAIRCLBMGXCZ-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO6S2

422

TRIS, MP Biomedicals™

TRIS, Molecular Biology Reagent, ≥99.95%, White Crystalline Powder

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Specifications

Storage Note 1	Store at Room Temperature (15-30°C). Store dessicated.
Molecular Weight (g/mol)	121.14
Loss on Drying	≤1%
Color	White
Grade	Molecular Biology Reagent
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
CAS	77-86-1
MDL Number	MFCD00004679
Solubility Information	Soluble in water (40% aq soln) Clear, Colorless Solution (550mg/mL), ethyleneglycol (79.1mg/mL), methanol (26mg/mL), anhydrous ethanol (14.6mg/mL), 95% ethanol (22.0mg/mL), DMF (14mg/mL), acetone (2mg/mL), ethyl acetate (0.5mg/mL), olive oil (0.4mg/mL), and chloroform (0.05mg/mL).
pH	10 to 11.5 (5% aq. soln.)
Synonym	Trometamol,THAM,Tromethamine,Tris base
Purity Grade Notes	≥99.95%
RTECS Number	TY2900000
Beilstein	741883
Molecular Formula	C4H11NO3
Melting Point	167°C to 172°C

423

Tris, MP Biomedicals™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

424

MilliporeSigma™ HEPES, Sodium Salt, ULTROL™ Grade, Calbiochem™,

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid, Na PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

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Specifications

PubChem CID	2724248
CAS	75277-39-3
Molecular Weight (g/mol)	260.28
ChEBI	CHEBI:46758
MDL Number	MFCD00036463
SMILES	[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Synonym	N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid, Na
IUPAC Name	sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO4S

425

BES, 99+%, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: n,n-bis 2-hydroxyethyl-2-aminoethanesulfonic acid,bes,ethanesulfonic acid, 2-bis 2-hydroxyethyl amino,2-bis 2-hydroxyethyl amino ethanesulfonic acid,n,n-bis 2-hydroxyethyl taurine,n,n-bis hydroxyethyl-2-aminoethanesulfonic acid,bes buffering agent,taurine, n,n-bis 2-hydroxyethyl,2-bis 2-hydroxyethyl amino-ethanesulfonicacid PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

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Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	n,n-bis 2-hydroxyethyl-2-aminoethanesulfonic acid,bes,ethanesulfonic acid, 2-bis 2-hydroxyethyl amino,2-bis 2-hydroxyethyl amino ethanesulfonic acid,n,n-bis 2-hydroxyethyl taurine,n,n-bis hydroxyethyl-2-aminoethanesulfonic acid,bes buffering agent,taurine, n,n-bis 2-hydroxyethyl,2-bis 2-hydroxyethyl amino-ethanesulfonicacid
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

426

N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, free acid, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N, N-Bis(2-hydroxyethyl)taurine,N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

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Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	N, N-Bis(2-hydroxyethyl)taurine,N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

427

HEPPS, MP Biomedicals™

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428

Thermo Scientific Chemicals AMPSO, 98+%

CAS: 68399-79-1 Molecular Formula: C7H17NO5S Molecular Weight (g/mol): 227.28 MDL Number: MFCD00041777 InChI Key: ACERFIHBIWMFOR-UHFFFAOYNA-N Synonym: N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid PubChem CID: 100211 IUPAC Name: 2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid SMILES: CC(C)(CO)NCC(O)CS(O)(=O)=O

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Specifications

PubChem CID	100211
CAS	68399-79-1
Molecular Weight (g/mol)	227.28
MDL Number	MFCD00041777
SMILES	CC(C)(CO)NCC(O)CS(O)(=O)=O
Synonym	N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid
IUPAC Name	2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid
InChI Key	ACERFIHBIWMFOR-UHFFFAOYNA-N
Molecular Formula	C7H17NO5S

429

MilliporeSigma™ TE Buffer, 100X, Molecular Biology Grade, Calbiochem™,

A 100X concentrate that, when diluted to 1X, contains 10mM Tris and 1mM EDTA

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430

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 8.0

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

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Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

431

ADA, 0.2M buffer soln., pH 6.5, Thermo Scientific™

CAS: 7415-22-7 Molecular Formula: C6H9N2NaO5 Molecular Weight (g/mol): 212.137 MDL Number: MFCD00065483 InChI Key: QCGKKVWCABJQQI-UHFFFAOYSA-M PubChem CID: 23679048 IUPAC Name: sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate SMILES: C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+]

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Specifications

PubChem CID	23679048
CAS	7415-22-7
Molecular Weight (g/mol)	212.137
MDL Number	MFCD00065483
SMILES	C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+]
IUPAC Name	sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate
InChI Key	QCGKKVWCABJQQI-UHFFFAOYSA-M
Molecular Formula	C6H9N2NaO5

432

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 8.0

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

433

Thermo Scientific™ TRIS, 1.0M buffer soln., pH 7.0

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

434

Thermo Scientific Chemicals HEPES hemisodium salt

CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	2724248
CAS	103404-87-1
Molecular Weight (g/mol)	260.28
ChEBI	CHEBI:46758
MDL Number	MFCD00036463
SMILES	[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Synonym	4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt
IUPAC Name	sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO4S

435

MOBS, 0.2M buffer soln., pH 8.5, Thermo Scientific™

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Specifications

CAS	115724-21-5
Molecular Weight (g/mol)	223.29
MDL Number	MFCD00674916
Color	Colorless
Physical Form	Liquid
pH	8.5
SMILES	OS(=O)(=O)CCCCN1CCOCC1
Synonym	4-(4-Morpholinyl)butanesulfonic acid
Recommended Storage	Keep cold
Concentration	0.2 M
IUPAC Name	4-(morpholin-4-yl)butane-1-sulfonic acid
InChI Key	VTOWJTPBPWTSMK-UHFFFAOYSA-N
Molecular Formula	C8H17NO4S

Life Science Buffers

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PIPES, Monosodium Salt, Biological Buffer, High Purity, 98.5%, Spectrum™ Chemical Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

PIPES, Monosodium Salt, Biological Buffer, High Purity, 98.5%, Spectrum™ Chemical