Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules MES Monohydrate | 145224-94-8 | MFCD00149409 | 100g
Chem-Impex | MES Monohydrate | 100g | 288406858 | 21885 | | 145224-94-8 | MFCD00149409 | 213.250 | C6H15NO5S
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Sigma Aldrich Fine Chemicals Biosciences MES hemisodium salt 98 tit100G
SODIUM MES hemisodium salt 98 tit100G
Research Products International Corp TAPS, 100 G
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 25 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications requiring precise pH regulation. ADA is commonly used in chromatography, protein studies, and enzyme assays, providing a stable pH environment during various biochemical experiments. Its ability to maintain a controlled pH is essential for accurate results in protein purification, cell culture, and molecular biology studies. The 25g size is perfect for smaller-scale experiments or routine laboratory use where reliable buffering capacity is necessary for optimal results.
Sigma Aldrich Fine Chemicals Biosciences MOPS BioUltra, for molecular biology, >=99.5% (T) | 1132-61-2 | MFCD00006183 | 500G
MOPS BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 500G
National Diagnostics MOPS-SDS Running Buffer (20X), 1L
Reliable and Economical, 18 Megohm Water, and 0.2 Micron Filtered. Ideal running buffer for high resolution SDS PAGE of proteins. 20X concentrate makes a working solution of 50mM Tris MOPS, 0.1% SDS and 1mM EDTA.
Biotang Inc HEPES Protein Extraction Buffer (5X), 500 ml
HEPES Protein Extraction Buffer (5X), 500 ml.