Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc TBS, 20X, PH7.4, 2000ML
20X Tris Buffer Saline is ready for dilution to a 1X working solution. Used in various research applications. One 2 Liter cubitainer.
Growcells PBS 1X 1L EA
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Kd Medical Inc PBS 20X Solution, PH 7.4, 1L
Phosphate Buffered Saline in a 20X concentrate for use as a wash buffer in immuno-labeling techniques. pH 7.4 One 1 Liter bottle.
Kd Medical Inc TRIS BUFFER, 2M,PH 8.0, BTL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. Tris Solution, 2M, provides a convenient stock solution for preparing Tris buffers> One 500 ml bottle.
Kd Medical Inc PBS 1X, PH 7.4 W/SOD AZIDE,20L
Phosphate Buffered Saline is used as a wash buffer in several lab applications. Contains sodium azide FACS washing grade, pH 7.4. One 20 liter cubitainer.
Growcells TAE BUFFER 50X 2L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
Kd Medical Inc TRIS-HCL, 1M, PH 7.4, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.4. One 500ml bottle.
Kd Medical Inc TRIS 1M, PH 8.5, 1000ML BTL
Buffer used in a wide range of Molecular Biology applications. 1 Molar solution pH 8.5. One 1 Liter bottle.
Kd Medical Inc TBE 10X 1 LITER BOTTLE
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 1 liter bottle.
Kd Medical Inc TRIS HCL 1M PH 8.O 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 8.0. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set Crystals, 250 g
Trizma Pre-set Crystals, 250 g.
Newcomer Supply Phosphate Buffer 0.1M, pH 7.2 to 7.4, 1 gallon
Use: IHC wash and rinse. Shelf Life is 1 year from date of manufacture. Store at 2-8°C.
Hampton Research HR2-729/1.0 M HEPES pH 7.5 - 100 ml
HR2-729/1.0 M HEPES pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 10L Sto
AdipoGen PBS Endotoxin-free (sterile) (3 x 10 ml)
PBS Endotoxin-free (sterile) (3 x 10 ml), Adipogen Life Sciences. Chemical. Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).