Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp Triton Lysis Buffer Solution, 500 ML
1X buffer used to lyse cells under non-denaturing conditions.
1X Formulation:
NaCI: 150 mM
TritonX-100: 1%
EDTA: 5 mM
Research Products International Corp MOPS Free Acid 50 G
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes.
Heavy Metals: <5 ppm
pKa: (20°C) 7.20 ± 0.2
pH Range: 6.5-7.9
Epigentek Inc TET ASSAY BUFFER (6ML)
The TET Assay Buffer is a component of Epigenase 5mC Hydroxylase TETActivity/Inhibition Assay Kit (Colorimetric)
Electron Microscopy Sciences Tris Buffer 0.2M, pH 8.0 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 8.0
Electron Microscopy Sciences Tris HCL Buffer, pH range 7.2-9.0 250 ML
Please specify pH when ordering.
pH: 7.2-9.0
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 250G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 250G
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt dry powder | 103404-87-1 | 10PAK
HEPES hemisodium salt dry powder | Mol Wt: 249.3 | 103404-87-1 | 10PAK
Teknova 2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 20X PBS with 1% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1X PBS, pH 7.2, 9mL Tubes, 25 pack, Sterile 12 pack.
137mM Sodium Chloride,4.3mM Disodium Phosphate, 2.7mM Potassium Chloride,1.4mM Monopotassium Phosphate,Adjusted to pH 7.2
Teknova 1M MOPS Buffer, pH 7.4, 1000mL, Sterile. 2 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Teknova 10mM Tris-HCl, pH 8.5. 2mL, 100-Pack, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova Organic Sperm Wash Buffer. 1000mL, DNase Tested, Sterile. 8 Pack.
50mM Sodium Chloride, 10mM Tris-HCl, 10mM Ethylenediaminetetraacetic acid (EDTA), 2.0% SDS
