Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
PROVISION KINETICS INC TRIS- SALINE BUFFER
500 ml bottle of Tris-saline buffer, pH 8.4 designed for use with Anti-IIa and Anti-Xa heparin test kits (sold separately)
American Research Products Inc TRIS-ACETATE BUFFER, 0.2M,
TRIS-Acetate Buffer, 0.2M, pH 7.8-500ml. 0.2M Tris base dissolved in 18.2 megohms water, pH adjusted to 7.8 with glacial acetic acid and filtered through 0.22-micron filter.
Biotang Inc Tris-Glycine Native Sample Buffer (4X), 25 ml
Tris-Glycine Native Sample Buffer (4X), 25 ml.
Biotang Inc Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
Alkali Scientific CellPro™ Tris Buffer-HCL, 1M, pH 8.0, 20L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
LAB BIOREAGENTS NGB I Buffer 100ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.
Kd Medical Inc TRIS GLYC SDS BUFFER, 10X, 4L
10X Tris Glycine SDS is used for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. One 4 liter cubitainer.
Polysciences, Inc. Tris Buffered Saline; Composition: 1M Tris-HCl, 3M Sodium chloride; 8.0+/-0.2 pH; Storage: 25 deg.C
Tris Buffered Saline; Composition: 1M Tris-HCl, 3M Sodium chloride; 8.0+/-0.2 pH; Storage: 25 deg.C
LAB BIOREAGENTS LowBinding I Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.