Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 1KG Stor
Bioworld PEB- Protein Extraction Buffer (5x), 250 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Waters Corp Tris CaCl2 Buffer Salts, pH 7.5, 4/pk
Waters Corporation Tris CaCl2 Buffer Salts, pH 7.5, 4/pk
Crescent Chemical Co Inc EDTA ANALYTICAL GRADE
Ethylenediamine tetraacetic acid analytical grade Size - 100G Storage Conditions - +15 °C TO +30 °C Catalog Number - 11278.01 CAS 60-00-4
Alkali Scientific TE Buffer, 1X, 500 Milliliters
TE Buffer, 1X, 500 Milliliters is a pre-made solution used for a variety of molecular biology applications that require a stable pH and buffering capacity. This formulation, specifically designed for use in nucleic acid purification, electrophoresis, and other biochemical assays, ensures that DNA and RNA samples are preserved and stabilized during handling. The 1X concentration is ready for immediate use, eliminating the need for dilution. It maintains an optimal pH and ionic strength to protect sensitive molecules from degradation, making it an essential component in any laboratory focusing on nucleic acid research and molecular diagnostics.
![Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502387606.png-250.jpg)
Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.
1.54M Sodium Chloride,55mM Sodium Phosphate Dibasic,12mM Potassium Phosphate Monobasic
Selleck Chemical LLC Bicine
Bicine (N N-Bis(2-hydroxyethyl)glycine Diethylolglycine Diethanol glycine Dihydroxyethylglycine) is an organic compound used as a buffering agent
Bioworld TAE Buffer, Quick-Mix Pack (25X), 1 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 25X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Bioworld TBS Buffer, Low Salt (10X), 1 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Bioworld STEBuffer (1X), 4 L
STE Reagent Common reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, ready to use 1X solution Technical Information:NaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 48 Grams of Powder, Makes 10 Liters of Solution
Murashige & Skoog Medium with MES Buffer is a plant tissue culture medium designed for larger-scale applications. The 10-liter solution, made from 48 grams of powder, is enhanced with MES buffer, which provides pH stability during plant cell culture. This medium is essential in plant biotechnology studies where pH control is crucial for ensuring the proper growth and development of plant cells. The MES buffer helps prevent pH fluctuations that could disrupt cell metabolism and growth. This formulation is widely used in laboratories involved in plant tissue culture, regeneration, and studies of plant cell development, offering a reliable solution for maintaining consistent pH levels.