Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC BES sodium | 66992-27-6 | 97.0% | 25 G
BES sodium is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is used in the diagnostic testing area.
- Biochemical buffer
- Effective pH range: 6.4 to 7.8 (pKa: 7.15 at 20 °C)
- Used in diagnostic testing
- Appearance: Solid
- Color: White to off-white
- Target: Biochemical assay reagents
- Pathway: Others
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659758.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 50 G
HEPES, Free Acid is a critical biochemical reagent for maintaining pH stability in cell cultures and biochemical experiments. This 50g package is ideal for smaller scale applications, where precise pH control is essential for the success of experiments. HEPES is effective in a pH range of 6.8 to 8.2, which makes it a preferred choice for a variety of applications including enzymatic assays, electrophoresis, and cell-based assays. Due to its excellent buffering capacity and low toxicity to cells, HEPES ensures that pH variations are minimized during extended incubation periods, supporting reliable and reproducible experimental outcomes.
Medchemexpress LLC 2-methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.4% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptor agonist, with EC50s of 19, 6.2, and 5 nM for human P2Y13, mouse P2Y13 and human P2Y12, respectively. It has pEC50s of 8.29 and 5.75 for human P2Y1 and rat P2Y6, respectively. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptor agonist
- Acts on human P2Y13, mouse P2Y13, human P2Y12, human P2Y1 and rat P2Y6 receptors
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1
eMolecules 7365-45-9 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | 7365-45-9 | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820
Bioworld CAPS, Transfer Buffer (5X), 500 mL
Product DescriptionCAPS-based transfer buffer at 5X contains a concentration between 90-110 mM CAPS and 10% methanol, with a pH value between 10.8-11.2 at 25C°. Buffer may be preferable for transfers of high molecular weight proteins (such as >150 kD) and in cases where the glycine component in buffer may interfere with downstream protein sequencing applications. Protease (P/F): None
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660290.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg
HEPES, Free Acid is a widely used biochemical reagent, available in a 25kg package for larger-scale applications. This highly stable buffer is effective in maintaining a pH range of 6.8–8.2, making it ideal for a variety of biochemical and biological applications such as protein analysis, cell culture, and drug formulation. HEPES helps maintain physiological pH levels, preventing changes that could interfere with experiments. This bulk package is especially beneficial for laboratories requiring frequent buffer preparation, allowing for efficient cost-saving and consistent results across a wide range of biological and chemical protocols.
Apexbio Technology LLC MES sodium salt 71119-23-8 1g
MES sodium salt (CAS 71119-23-8) is a buffering agent designed to maintain stable pH conditions in the range of 5 5 7 0 supporting physiological experimental environments MES sodium salt does not have a direct cellular or molecular target and is not a small-molecule inhibitor agonist or antagonist Its key role is pH stabilization in various biological and biochemical assays including enzymatic activity assays receptor pathway investigations and isolation of cellular organelles or biomolecules Experimental models include plant cell culture systems and physiological in vitro assays Based on these properties MES sodium salt holds research potential in applications such as plant cell culture controlled metabolic pathway studies and signal transduction research requiring consistent pH conditions
Biotang Inc HEPES-Buffer, 0.5M pH 7.6, 100ml 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 7.6 +/- 0.15. BB-2078
HEPES-Buffer, 0.5M pH 7.6, 100ml 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 7.6 +/- 0.15. BB-2078.
![Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00116527-BIOTANGBIOLOGICAL-BUFFERS.JPG-250.jpg)
Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm
MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661234.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 100 G
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a biochemical reagent supplied in a 100G package. It is used primarily for preparing buffered solutions in biological and chemical experiments. PIPES is highly stable across a broad pH range and is especially useful for maintaining pH in experiments involving biological systems such as enzymes, cells, and proteins. It is commonly utilized in electrophoresis, chromatography, and other biochemical processes where pH control is crucial. The sesquisodium form of PIPES ensures that solutions maintain consistent acidity or basicity without interference with the biological samples under study.
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 250 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255311.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 250 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
Bioworld ACES Buffer, 0.5M, pH 6.5, 500 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 6.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Gold Biotechnology Inc HEPPSO 100 g
HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.
Medchemexpress LLC Tapso | 68399-81-5 | 98.0% | 100 G
TAPSO is a widely used biological buffer and pH stabilization reagent. It offers an effective pH range between 7 and 8, with pKa values typically falling between 7.5 and 9.0. This compound contains Tris groups and has been observed to exhibit reactive activity with zwitterionic glycine peptides. It is primarily intended for research applications.
- Effective pH range of 7-8.
- pKa values ranging from 7.5-9.0.
- Contains Tris groups.
- Shows reactive activity with zwitterionic glycine peptides.
- Suitable for various research applications.