Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules N-Cyclohexyltaurine | Combi-Blocks | 103-47-9 | MFCD00003835 | 207.290 | C8H17NO3S | 98.000 | OS(=O)(=O)CCNC1CCCCC1 | 100g | 290700396
N-Cyclohexyltaurine | Combi-Blocks | 103-47-9 | MFCD00003835 | 207.290 | C8H17NO3S | 98.000 | OS(=O)(=O)CCNC1CCCCC1 | 100g | 290700396
Biotang Inc MOPS-EDTA-Sodium Acetate Buffer (MESA), 500 ml
MOPS-EDTA-Sodium Acetate Buffer (MESA), 500 ml.
Biotang Inc RNAse Free Phosphate-Buffered Saline, 250 ml
RNAse Free Phosphate-Buffered Saline, 250 ml.
Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm
MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm.
Sigma Aldrich Fine Chemicals Biosciences PIPES Dipotassium Salt | 108321-27-3 | MFCD00069751 | 100g
PIPES Dipotassium Salt | Purity: 99% | Mol Wt: 378.55 | 108321-27-3 | MFCD00069751 | 100g
Medchemexpress LLC 2-methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.4% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptor agonist, with EC50s of 19, 6.2, and 5 nM for human P2Y13, mouse P2Y13 and human P2Y12, respectively. It has pEC50s of 8.29 and 5.75 for human P2Y1 and rat P2Y6, respectively. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptor agonist
- Acts on human P2Y13, mouse P2Y13, human P2Y12, human P2Y1 and rat P2Y6 receptors
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 25 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier.
- Buffering agent with a useful pH range from 7.3 to 8.7.
- Reduces Aβ-aggregate-induced memory deficits.
- Rescues cognitive deficits in mice.
- Orally active.
- Penetrates the blood-brain barrier.