Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Supply Solutions Sigma-Aldrich -500ml Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0 (Fluka) * Third Party Item
Sigma-Aldrich -500ml Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0 (Fluka) * Third Party Item
S2 Media 20x Tris Buffered Saline (400mM Tris-HCl, 3M NaCl), pH 7.4, Sterile, 1L Media Bottle, 1ea
Tris Buffered Saline (TBS), formulated at a 20x concentration, is used in cell culture, molecular biology, protein research, and purification applications. It can be utilized as a wash buffer, storage buffer, and for resuspension of cells. he Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration.• Tris Buffered Saline should be used by trained professionals within a laboratory setting. • Tris is an abbreviation for tris(hydroxymethyl)aminomethane).• Product is not intended for use in the diagnosis or treatment of disease or other human conditions.• For Research Use Only (RUO). Not for use in diagnostic procedures.
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eMolecules benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g
Pharmablock | benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g | 551109237 | PBJP030 | 305.374 | C17H23NO4
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eMolecules 220598-43-6 | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | MFCD18073347 | 1g
Ambeed | (4-((4-(tert-Butoxycarbonyl)piperazin-1-yl)sulfonyl)phenyl)boronic acid | 250mg | 731676797 | A260715 | 486422-54-2 | MFCD28384269 | 370.230 | C15H23BN2O6S
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Biocare Medical, LLC Tris Buffer Saline (TBS) Plus, 10X
For laboratory use and procedures requiring a high quality Tris buffer saline. TBS is sodium azide and thimerosal free and provides superior pH stability. It can also be used for manual immunohistochemistry applications or on an automated staining system with the addition of surfactant.
Biotang Inc TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
Biotang Inc TRIS-Buffered Saline (10X, Low Salt), 1000 ml
TRIS-Buffered Saline (10X, Low Salt), 1000 ml.