Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC One-step TUNEL Cy5 Apoptosis Detection Kit 20rxns
One-step TUNEL Cy5 Apoptosis Detection Kit is designed for detecting cell nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases are activated cleaving genomic DNA between nucleosomes and resulting in DNA fragmentation into segments approximately 180-200 bp or multiples thereof The fragmented genomic DNA contains exposed 3 -OH ends which are labeled with fluorescent-tagged dUTP in a TdT (Terminal Deoxynucleotidyl Transferase) catalyzed reaction allowing visualization and detection by fluorescence microscopy or flow cytometry (Ex/Em 649 nm/670 nm) This kit has a broad range of applications and can be utilized to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells
Med Vet International Lisinopril Usp Tablets, 20mg, 100ct
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
Lisinopril is used to treat high blood pressure (hypertension) in adults and children who are at least 6 years old. Lisinopril is also used to treat congestive heart failure in adults, or to improve survival after a heart attack.
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 500mL
CellPro™ DPBS Buffer, offered in a 1X concentration and a convenient 500mL volume, provides a balanced salt solution essential for numerous cell culture and molecular biology procedures. Its molecular biology grade ensures high purity and reliability in maintaining cellular health and integrity during experiments.
Quality Biological Inc PBS pH 7.2, CS 10 X 500 mL
PBS (phosphate buffered saline) is a balanced salt solution ¿ Used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents ¿ Endotoxin and Mycoplasma tested ¿ Also useful in immunological applications, such as Western Blotting ¿ Storage Condition: 15¿C ¿ 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.1 -7.3 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline1L
Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures as well as during sample preparation of cell lysates for use in general molecular biology applications.
Gold Biotechnology Inc MES Free Acid 250 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.0, pH Adjusted with KOH) - 250ml
HEPES Buffer (0 5 M pH 7 0 pH Adjusted with KOH) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MOPS-EDTA Sodium Acetate, MESA (10X, RNase Free) - 1L
MOPS-EDTA Sodium Acetate MESA (10X RNase Free) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc PIPES Buffer (1 M, pH 7.0) - 500ml
PIPES Buffer (1 M pH 7 0) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
Hampton Research HR2-902-10/StockOptions HEPES #10 pH 7.7, 185 ml
HR2-902-10/StockOptions HEPES #10 pH 7.7, 185 ml. Please note items from this supplier are non-returnable.