Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
UNITED STATES BIOLOGICAL CORPORATION DULBECCO MEM W/O GLUCOSE 25L
NC3790589 DULBECCO MEM W/O GLUCOSE 25L
Boston Bioproducts Inc Tris-Base (2 M, pH > 10.0) - 250ml
Tris-base is a widely used biological buffer in various research applications in biochemistry, molecular biology, and cell biology. The buffering capabilities of Tris-base are effective in the pH range of 7.0-9.0, making it suited for various biological applications. This buffer is widely used in protocols for DNA and RNA extraction, in situ hybridization, protein extraction from cells, in agarose gel electrophoresis, polyacrylamide gel electrophoresis and as a wash buffer in Western blotting.
LAB BIOREAGENTS NGB I Buffer 100ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.
LAB BIOREAGENTS LowBinding V Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerforman1L
MOPS SOLUTIONon BioPerforman1L
Sigma Aldrich Fine Chemicals Biosciences MOPS-EDTA-Sodium Acetate M1L
MOPS - EDTA - Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum reSOLUTIONon.
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-1000.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 1 Kilogram
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Sigma Aldrich Fine Chemicals Biosciences MES Monohydrate | 145224-94-8 | MFCD00149409 | 100g
MES Monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 100g
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.