Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TAE Buffer (10X), 1 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 10X concentration and is prepared with 18 megohm water. It has a pH of 8.3 ± 0.2. A concentration of 1X contains 0.4M Tris acetate and 0.01M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Sigma Aldrich Fine Chemicals Biosciences MOBS >=99% (titration) | 115724-21-5 | MFCD00674916 | 25G
MOBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 223.29 | 115724-21-5 | MFCD00674916 | 25G
Seracare Life Sciences Inc 10X TRIS-GLYCINE-SDS BUFFER
Tris-Glycine-SDS (TGS) is a running buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. pH=8.3. Conductivity controlled.
![Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659834.jpg-250.jpg)
Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 100 Grams
BIS TRIS Propane (1,3 Bis (TRIS(hydroxymethyl)methylamino)propane) is a biochemical reagent used as a buffering agent in laboratory research. The 100g quantity provides enough material for numerous experiments that require pH stability in a range of biological and chemical applications. BIS TRIS Propane is particularly valuable for creating buffers with a stable pH in the physiological range, making it ideal for protein studies, enzymatic assays, and nucleic acid work. It is commonly used in molecular biology, microbiology, and biochemistry for maintaining consistent conditions during reactions. This reagent is a reliable choice for labs that require precise control over pH to ensure the integrity of their experiments.
![Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659752.jpg-250.jpg)
Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 500 G
BIS TRIS Propane (1,3 Bis (TRIS(hydroxymethyl)methylamino)propane) is a high-purity biological buffer commonly used in laboratory applications requiring precise pH control. This powder form of the buffer is highly soluble in water, allowing for easy preparation of buffered solutions with specific pH values. It is frequently used in protein electrophoresis, chromatography, and enzyme assays where stable pH is crucial for maintaining the activity and structure of biological molecules. The high purity of BIS TRIS Propane ensures minimal interference in sensitive biochemical analyses, making it ideal for use in research and clinical laboratories where accuracy is essential. The 500g size provides an ample supply for routine laboratory procedures.
MOLTOX Molecular Toxicology Inc 0.1M Tris-HCL Buffer, pH 8.0, 1000 mL/bottle, Store at Room Temperature
MOLTOX manufactures a wide variety of buffers for your laboratory needs. All MOLTOX reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Growcells TAE BUFFER 50X 2L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
Kd Medical Inc TRIS-HCL, 1M, PH 7.4, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.4. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences Tris Acetate-EDTA buffer B1L
TRIS Tris Acetate-EDTA buffer B1L
Quality Biological Inc Tris HCL (1 M), pH 8.0, 500 mL
A hybridization buffer specifically formulated for use with nylon membranes. ¿ It does not contain Denhardt¿s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. ¿ Formulated for Nylon membrane ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase, and Protease tested ¿ Applications: Denatured fragmented salmon sperm DNA may be added to this hybridization buffer to reduce nonspecific background. ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Boston Bioproducts Inc Tris-Acetate Buffer (0.2 M, pH 7.4) - 500ml
The Tris-Acetate Buffer is a buffering agent composed of Tris-base and acetic acid. The combination of these ingredients yields a pH range of 6.0-8.0. This buffer is ideal for requiring a pH slightly above physiological conditions. Often used to support the electrophoresis of nucleic acids on agarose gel and protein electrophoresis employing SDS-PAGE and related blotting techniques.
Sigma Aldrich Fine Chemicals Biosciences TRIS Glycine buffer soluti1L
TRIS Glycine buffer SOLUTION1L