Methyl Green is a non-intercalating fluorescent labeling agent that selectively binds to the major groove of DNA. It electrostatically interacts with the major groove of DNA through positively charged groups, exhibiting high affinity, resistance to photobleaching, and stable fluorescence emission. It can be directly measured by microscopy and flow cytometry, with peaks at 633 and 677 nm.
- Selectively binds to the major groove of DNA
- Exhibits high affinity and stable fluorescence emission
- Resistant to photobleaching
- Directly measurable by microscopy and flow cytometry
- Used for fluorescent labeling of nuclei
- Applicable for DNA staining and cell activity detection in gel electrophoresis
- Suitable for flow cytometry to detect cell viability
- Useful for fluorescence spectroscopy in DNA binding analysis