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Assay plates (typically polystyrene) whose well surfaces are pre-coated with proteins or other biomolecules to enable affinity binding and assay detection of specific molecules from samples; includes surface-activated plates for covalent binding.
YT Microplate, Appearance: Clear To Opaque Dry Material Adhering To Internal Well Surfaces, Solubility: Clear Solution At 1x Concentration, Extinguishing Media: Waterspray, Stability: Stable.
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Application: This Streptavidin (SA) Coated Plate is intended for Immunoassay and ELISA. The plate could be separated to 12× 8-well strips and the frame is white. Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications.
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Lipoprotein Lipase (LPL) is the key plasma lipase responsible for the hydrolysis of the triglyceride core in VLDL and chylomicrons. Cell Biolabs' Lipoprotein Lipase Activity Assay provides a convenient assay to quantify LPL activity in plasma, serum, and lysates. LPL activity levels are quantified in a 96-well fluorescence plate reader. This kit also detects endothelial + hepatic lipase activity. Each kit provides sufficient reagents for 100 assays including standards and unknown samples.
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bioWorld's Glutathione Coated Plates are standard 96-well polystyrene microplates whosesurface has been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. Applications:Analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.
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Nickel-Coated Microplates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear plates can be used with colorimetric detection methods. Specifications:Suitable for ELISA bases studiesBinding Capacity: 5-10 pmols/well for His-tagged protein
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Jacalin, belonging to the lectins family, is a Hemagglutinin from the seeds of the jack fruit Artrocarpus integrifolia. It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with...
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