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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (33,744)
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136 – 150 of 33,003 results
136

Thermo Scientific™ POROS™ Heparin 50 μm Affinity Columns
GREENER_CHOICE

Thermo Scientific™ POROS™ Prepacked Heparin, Affinity Column. POROS Heparin is based on an immobilized Heparin functional group designed for high throughput purification of proteins or viruses with specific Heparin affinity.

Particle Size 50 μm
Stationary Phase Heparin (Porcine)
Column Type Affinity
Quantity 1 Column
Material PEEK
Max. Pressure 750 psi (50 bar)
Product Line POROS
137

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-8 HPLC Column, 5 μm Particle Size

Provides less retention of both polar and non-polar compounds than C18.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
138

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18 HPLC Column, 5 μm Particle Size

A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
139

MilliporeSigma™ Supelco™ Kromasil C18 HPLC Column, 100 Å, 5 μm Particle Size

Kromasil C18 HPLC Column, 100 Å, 5 μm Particle Size for small molecules and peptides.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Product Type HPLC Column
Packing Material Silica-based Monomeric
Product Line Kromasil
140

TSKgel™ G3000SW HPLC Column, 13 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G3000SW SEC columns provide excellent separation of proteins and other biomolecules with molecular weights up to 500,000 Da.

Particle Size 13 μm
Solvents 0 to 100% aqueous soluble organic solvents
Flow Rate 8 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 21.5 mm
pH 2.5 - 7.5
Product Line TSKgel
USP Type L20, L59
141

TSKgel™ SuperHM-L HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

SuperHM-L Mixed-Bed Columns are packed with rigid polymer beads in varying pore sizes. They are used for the non-aqueous separation of polymers with a broad molecular weight range, or for analyzing unknown samples.

142

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-8-DB HPLC Column, 5 μm Particle Size

SUPELCOSIL™ LC-DB phases are specially deactivated for basic compounds. These columns provide less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
143

TSKgel™ Amide-80 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase Carbamoyl
Temperature 10°C to 50°C
Max. Pressure 200 bar
Product Line TSKgel
USP Type L68
144

TSKgel™ SuperSW mAb HR HPLC Column, 4 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).

Pore Size 250 Å
Particle Size 4 μm
Solvents 0 to 100% aqueous soluble organic solvents
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
pH 2.5 - 7.5
Material Stainless Steel
Product Line TSKgel
USP Type L20, L59
145

MilliporeSigma™ Supelco™ Ascentis™ Express RP-Amide HPLC Column, 2.7 μm Particle Size

Ascentis™ Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis™ Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from 'phase collapse'.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
146

TSKgel™ G5000PW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G5000PW SEC columns can be used for the analysis of water-soluble polymers with a molecular weight of less than 1,000,000 Da.

Pore Size 1000 Å
Particle Size 17 μm
Solvents ≤20% organic solvent
Flow Rate 1.2 mL/min.
Stationary Phase Hydroxylated Methacrylate
Temperature 80°C
Phase Size Exclusion
Matrix Polymer Particle Platform
Diameter (Metric) 7.5 mm
pH 2.0 - 12
Product Line TSKgel
USP Type L38, L39
147

TSKgel™ UP-SW3000 HPLC Column, 2 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ UP-SW3000 columns deliver dramatically increased resolution between fragments, monomers, and aggregates.

Pore Size 250 Å
Particle Size 2 μm
Solvents 0 to 30% aqueous soluble organic solvents
Flow Rate 0.35 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 4.6 mm
pH 2.5 - 7.5
Max. Pressure 340 bar
Product Line TSKgel
USP Type L20, L59
148

Thermo Scientific™ POROS™ CaptureSelect™ FcXL Affinity HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ FcXL Affinity HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect affinity ligands.

Shipping Condition Wet Ice
Pore Size 2000 Å
Stationary Phase IgG Fc affinity ligand, human‐specific
Temperature 2-40°C
Column Type Affinity
Quantity 1 Column
Material PEEK
Max. Pressure 2400 psi (170 bar)
Column Format Analytical Column, Biocompatible PEEK
Particle Size 20 μm
Packing Material Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
Binding Capacity 7.3 mg/mL
pH 1-10
Technique Bio-LC
Product Line POROS CaptureSelect
149

MilliporeSigma™ SeQuant™ ZIC™-pHILIC Polymeric Analytical PEEK Guard Columns

PEEK coated column

Pore Size 200 Å
Particle Size 5 μm
Length (Metric) 20 mm
Product Type Guard Column
Diameter (Metric) 2.1 mm
Porosity 200 Å
150

Thermo Scientific™ HyPURITY™ Cyano HPLC Columns

HyPURITY HPLC Columns are highly characterized, tested, and validated.

Particle Size 5 μm
Stationary Phase Cyano (CN)
Packing Material Silica
Column Type Mixed Mode
Diameter (Metric) 4.6 mm
Surface Area 200 m²/g
USP Type L10