
HPLC Columns and Cartridges
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MilliporeSigma™ Supelco™ Ascentis™ Express HILIC HPLC Column, 2.7 μm Particle Size
Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures.
Pore Size | 90 Å |
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Particle Size | 2.7 μm |
Stationary Phase | Silica |
Phase | Reversed Phase |
Matrix | Silica Phase/Fused-Core Particle Platform |
Endcapped | No |
pH | 2 to 8 |
Technique | Fused-Core HPLC |
Product Line | Ascentis |
MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size
Ascentis™ Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Pore Size | 90 Å |
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Particle Size | 5 μm |
Stationary Phase | PFP (Pentafluorophenyl) |
Phase | Reversed Phase |
Matrix | PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform |
Endcapped | Yes |
pH | 1 to 9 |
Technique | Fused-Core HPLC |
Product Line | Ascentis |
MilliporeSigma™ Supelco™ Ascentis™ Express ES-Cyano HPLC Column, 5 μm Particle Size
The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.
Pore Size | 90 Å |
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Particle Size | 5 μm |
Stationary Phase | Diisopropyl-Cyanopropylsilane |
Phase | Reversed Phase |
Matrix | Diisopropyl-Cyanopropylsilane Phase/Fused-Core Particle Platform |
Endcapped | Yes |
pH | 2 to 9 |
Technique | Fused-Core HPLC |
Product Line | Ascentis |
TSKgel™ Amide 80 HPLC Column, 2 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ Amide-80 columns are used for the analysis of saccharides, glycans, oligosaccharides, peptides and polar compounds from natural products.
Particle Size | 2 μm |
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Stationary Phase | Carbamoyl |
Temperature | 50°C |
Phase | HILIC |
Matrix | Spherical Silica |
pH | 2.0 - 7.5 |
Material | Stainless Steel Hardware |
Surface Area | 450 m2/g |
Max. Pressure | 150 bar |
Product Line | TSKgel |
USP Type | L68 |
TSKgel™ G2000SW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ G2000SW SEC columns provide excellent separation of peptides and proteins with molecular weights up to 100,000 Da.
Pore Size | 125 Å |
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Particle Size | 10 μm |
Solvents | 0 to 100% aqueous soluble organic solvents |
Flow Rate | 1.2 mL/min. |
Stationary Phase | Diol |
Temperature | 10°C to 30°C |
Phase | Size Exclusion |
Matrix | Silica Particle Platform |
Diameter (Metric) | 7.5 mm |
pH | 2.5 - 7.5 |
Product Line | TSKgel |
USP Type | L20, L59 |
TSKgel™ G5000PW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ G5000PW SEC columns can be used for the analysis of water-soluble polymers with a molecular weight of less than 1,000,000 Da.
Pore Size | 1000 Å |
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Particle Size | 17 μm |
Solvents | ≤20% organic solvent |
Flow Rate | 1.2 mL/min. |
Stationary Phase | Hydroxylated Methacrylate |
Temperature | 80°C |
Phase | Size Exclusion |
Matrix | Polymer Particle Platform |
Diameter (Metric) | 7.5 mm |
pH | 2.0 - 12 |
Product Line | TSKgel |
USP Type | L38, L39 |
TSKgel™ PW-Type Guard Column, 13 μm Particle Size, Sold by MilliporeSigma™ Supelco™
For use with TSKgel™ Columns, shipped in water.
Particle Size | 13 μm |
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Solvents | ≤ 20% Organic Solvent |
Flow Rate | 1.2 mL/min |
Stationary Phase | Hydroxylated Methacrylate |
Description | Salt Concentration: ≤ 0.5 M |
Length (Metric) | 7.5 cm |
Packing Material | Polymer Particle Platform |
Diameter (Metric) Inner | 7.5 mm |
pH | 2 to 12 |
Technique | Size Exclusion (SEC) |
Temperature (Metric) Maximum | 80°C |
Product Line | TSKgel |
USP Type | L38, L39 |
TSKgel™ ODS-120A HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ ODS-120A columns contain 150 Å pore size silica particles. The exclusion limit of 10,000 Da makes TSKgel™ ODS-120A columns a good choice for the reversed phase chromatography of peptides, small proteins, and environmental samples such as poly-aromatic hydrocarbons.
Pore Size | 150 Å |
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Particle Size | 10 μm |
Solvents | 0 to 100% organic solvent |
Carbon Load | 20% |
Stationary Phase | C18 (octadecyl) |
Temperature | 0°C to 45°C |
Phase | Reversed Phase |
Matrix | Spherical Silica |
Length (Metric) | 30 cm |
Endcapped | No |
pH | 2.0 - 7.5 |
Product Line | TSKgel |
USP Type | L1 |
TSKgel™ G3000PW HPLC Column, 12 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ G3000PW SEC columns can be used for the analysis of water-soluble polymers of less than 50,000 Da molecular weight.
Pore Size | 200 Å |
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Particle Size | 12 μm |
Solvents | ≤20% organic solvent |
Flow Rate | 1.2 mL/min. |
Stationary Phase | Hydroxylated Methacrylate |
Temperature | 80°C |
Phase | Size Exclusion |
Matrix | Polymer Particle Platform |
Diameter (Metric) | 7.5 mm |
pH | 2.0 - 12 |
Product Line | TSKgel |
USP Type | L37, L38, L39 |
TSKgel™ ODS-120A HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ ODS-120A columns use a 150 Å pore size silica base support. The silica′s exclusion limit of 10,000 Da makes them a good choice for the reversed phase chromatography of peptides, small proteins, and environmental samples such as poly-aromatic hydrocarbons.
Pore Size | 150 Å |
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Solvents | 0 to 100% organic solvent |
Stationary Phase | C18 (octadecyl) |
Phase | Reversed Phase |
Endcapped | No |
Surface Area | 200 m2/g |
Particle Size | 5 μm |
Flow Rate | 1.2 mL/min. |
Carbon Load | 20% |
Matrix | Silica Particle Platform |
Diameter (Metric) | 4.6 mm |
pH | 2.0 - 7.5 |
Product Line | TSKgel |
USP Type | L1 |
TSKgel™ G2500PW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Use TSKgel™ G2500PW for the analysis of water-soluble polymers with molecular weights of less than 3,000 Da.
TSKgel™ Phenyl-5PW RP HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ Phenyl-5PW RP columns are filled with 10 micron porous polymethacrylate particles, the surface of which is covered with a high density of phenyl groups that causes proteins to be retained in a low salt buffer mobile phase and requiring a solvent gradient for their elution, as is the protocol in reversed phase chromatography.
TSKgel™ G2000SWXL HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel™ SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
TSKgel™ SP-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel SP-NPR columns are packed with non-porous, hydrophilic polymer beads which are surface modified with a strong cation exchanger. They are used for the separation and analysis of proteins and peptides.
TSKgel™ Boronate-5PW HPLC Guardgel Kit, Sold by MilliporeSigma™ Supelco™
Boronate-5PW HPLC guardgel kit
Particle Size | 20 μm |
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Stationary Phase | M-aminophenyl boronate phase |
pH | 2 to 9 |
Technique | Affinity |
Column Capacity | 40 μmol/mL, resin adsorption capacity (sorbitol) |