HPLC Columns and Cartridges
Waters Corporation Atlantis T3 Column, 100Å, 3 μm, 3 mm x 50 mm
Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns providing balanced retention of polar and hydrophobic molecules. They are a primary choice for developing separations of polar and non-polar compounds. Columns offer extended longevity at low pH and improved peak shape for amine-containing bases at pH 7, ensuring enhanced performance across a broad pH range. They retain and separate highly polar chemicals, offering strong retention and selectivity for neutral and hydrophobic chemicals in addition to polar analytes.
- Universal, silica-based, reversed-phase C18 column.
- Balanced retention of polar & hydrophobic molecules.
- Extended longevity; improved peak shape at low pH.
- Effective over wide pH range (2-8 pH).
- T3 bonding for polar retention, aqueous compatibility.
- Patented T3 end-capping for enhanced efficiency.
- Max pressure: 6000 psi (415 Bar).
Waters Corp Waters Atlantis T3 Column, 100 Å, 3 μm, 3 mm x 100 mm
Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns for balanced retention of polar and hydrophobic molecules, ideal for developing separations of polar and non-polar compounds.
- T3 bonding technology promotes polar compound retention and mobile phase compatibility with a trifunctional C18 alkyl phase.
- Efficient end-capping is superior to traditional trimethyl silane (TMS) end-capping.
- Superior performance offers enhanced column performance, improved lifetime, peak shape, and stability.
- HPLC methods can be easily transferred to UPLC methods due to T3 bonding.
- Extended column lifetimes under low pH conditions; T3 bonding protects the silica substrate from dissolution.
Waters Corp Waters XBridge BEH C8 Column, 5 μm, 4.6 mm × 250 mm
The XBridge BEH C8 column offers less retention than C18, improving peak shape and chromatographic performance for faster method development across a wide pH range (1 to 12).
- Trifunctionally bonded sorbent provides superior low pH stability and ultra-low column bleed.
- Offers unmatched potential column lifetime and flexibility across mobile phase pH and temperatures.
- Enables robust separations and seamless migration to UPLC technology.
- Proprietary BEH Technology facilitates scaling across UPLC, analytical, and preparative platforms.
- Features reduced USP peak tailing, increased chemical/mechanical stability, and high pH stability.
- Each batch is chromatographically tested for excellent, reproducible performance.
- Manufactured in a cGMP, ISO 9001:2000 certified plant using ultra-pure reagents.
Waters Corp Waters Column, C18, 130Å, 3.5 μm, 3 mm × 150 mm
The XBridge BEH C18 sorbent serves as a workhorse for HPLC method development, offering compatibility across a mobile phase pH range of 1-12. This column features a trifunctionally bonded C18 ligand, providing universal utility for most HPLC separations, with superior low pH stability and ultra-low column bleed.
- Compatible with mobile phase pH from 1 to 12.
- Trifunctionally bonded C18 ligand for universal HPLC separations.
- Provides superior low pH stability and ultra-low column bleed.
- Allows operation under various mobile phase, temperature, and pH conditions.
- Offers maximum stability under extreme pH conditions.
- Minimizes LC/MS bleed and enhances hydrolytic stability and particle strength.
- Optimized for control over analyte retention and selectivity.
Waters Corp Waters XBridge BEH Shield RP18 Column, 3.5 μm, 3 mm x 150 mm
XBridge BEH Shield RP18 columns are designed to complement XBridge BEH C18 and C8 phases, offering an alternative selectivity, particularly for phenolic compounds, due to an embedded carbamate group in the bonded phase ligand. They provide a powerful tool for HPLC method development.
- C18 chemistry for reversed phase separation
- Hybrid particle substrate
- Broad pH range from 2 to 11
- Withstands maximum pressure of 6000 psi (415 Bar)
- Endcapped design
- Features Shield RP18 bonding technology
- Low silanol activity
- Spherical particle shape
- Particle size of 3.5 μm
- Pore size of 130 Å
- Optimized for HPLC systems
- Utilizes BEH particle technology
- Meets USP L1 classification
- Dimensions of 3 mm inner diameter and 150 mm length
- 17% carbon load
- Supplied as 1 unit per package
Waters Corporation Column, XBridge BEH Shield RP18, 130Å, 3.5 μm
These BEH Shield RP18 columns are designed for high-performance liquid chromatography (HPLC) method development, offering alternate selectivity for compounds like phenolics. They deliver excellent peak shape, high efficiency, and stability across acidic and basic mobile phases, enabling robust and confident separations through advanced bonding and particle technology.
- Complements C18/C8 phases for HPLC method development with alternate selectivity.
- Embedded carbamate group provides specific selectivity for phenolic compounds.
- Achieves excellent peak shape, high efficiency, and stability in acidic/basic mobile phases.
- Shield Technology ensures stable retention in 100% aqueous mobile phases.
- BEH particle offers wide usable pH range (pH 1–12) with superior low pH stability.
Waters Corp XBridge BEH Shield RP18 Column, 130Å, 3.5 μm, 4.6 mm, 100 mm
These columns are designed to complement BEH C18 and C8 phases, providing an essential tool for HPLC method development. They feature an embedded carbamate group in the bonded phase ligand, which offers alternate selectivity, particularly for phenolic compounds, compared to traditional straight-chain alkyl columns.
- Alternate selectivity for phenolic compounds with embedded carbamate group.
- Improved peak shape by reducing silanol interactions with basic analytes.
- Robust separations through full utilization of pH.
- Superior pH stability over widest range (1 to 12) for method ruggedness.
- Enhanced productivity, maximizing particle efficiency and column lifetime.
- Unmatched reproducibility through controlled manufacturing and testing.
Waters Corp Waters XBridge BEH C8 Column, 3.5 μm, 4.6 mm ID, 100 mm Length
The XBridge BEH C8 sorbent is less retentive compared to the XBridge BEH C18 sorbent, offering speed, improved peak shape, and chromatographic performance. The trifunctionally bonded sorbent provides the widest usable pH range (1 to 12), superior low pH stability, and ultra-low column bleed.
- Less retentive compared to BEH C18 sorbent.
- Offers the widest usable pH range (1 to 12).
- Superior low pH stability.
- Ultra-low column bleed.
Waters Corp Waters SunFire C18 Column, 100Å, 2.5 μm, 4.6 mm X 50 mm
These C18 columns are designed for general purpose method development at low pH, featuring high retention for basic compounds and suitability for purification and impurity assays.
- High retention for basic compounds; ideal for purification and impurity profile assays.
- Superior low pH stability extends column lifetime.
- Patented synthesis and bonding increase efficiency and sensitivity.
- Effective design eliminates method reoptimization.
- Comparable performance for scaling up analytical to preparative columns.
- Achieves sharp peaks, outstanding sensitivity, and minimal bleed in MS applications.
- Superior resolution, speed, and low back pressure reduce cost and analysis time.
- Produces symmetrical peaks for better quantification and resolution of substances across pH ranges.
Waters Corp Waters Prep Column, Silica, OBD, 100Å, 5 μm Particle
The SunFire Prep OBD Silica columns are preparative columns specifically designed for use under normal phase conditions. They feature an Optimum Bed Density (OBD) hardware format, which significantly extends column lifetime and ensures robust performance across a wide range of operating parameters.
- Optimum Bed Density (OBD) hardware provides superior column lifetime and robust performance.
- Normal-phase separation mode for chromatographic applications.
- High silanol activity for specific chromatographic selectivity.
- Broad pH range of 2 to 8 for versatile sample matrices.
- High maximum pressure (6000 psi / 415 Bar) compatible with preparative HPLC systems.
- Spherical silica particle shape (5 μm) ensures efficient separations.
- 100 Å pore size optimized for a range of analytes.
Waters Corp Waters Column, C18, 5 μm, 4.6 mm x 100 mm, 100 Å
These reversed-phase columns incorporate Waters’ patented embedded polar group technology, which “shields” the silica’s residual silanol surface from highly basic analytes. This reduced silanol activity significantly improves peak shape and resolution.
- Utilizes patented embedded polar group technology to shield against basic analytes.
- Provides improved peak shape and resolution across a broad pH range.
- Retains basic chemicals less than conventional C18 and C8 columns.
- Offers tight specification ranges for outstanding reproducibility, minimizing resolution shifts.
- Produces more consistent results from batch to batch and column to column.
- Long-lasting due to hydrolytic stability and mechanical stability of packed bed, lowering lab costs.
- Provides a wide pH range (2 to 8 pH) for optimization at neutral or low pH.
Waters Corp Waters OBD Prep Column, C8, 100Å, 5 μm, 30 mm × 75 mm
SunFire C8 columns are used for general purpose method development at low pH. The C8 ligand is preferred for some assays because it is less hydrophobic and therefore less retentive compared to SunFire C18.
- General purpose method development at low pH
- Less hydrophobic and less retentive compared to C18
Waters Corp Waters XBridge BEH C8 OBD Prep Column, 130Å, 5 μm, 30 mm X 50 mm
The XBridge BEH C8 sorbent is less retentive than the C18, offering faster analysis. It's designed for method developers seeking improved peak shape and chromatographic performance. This trifunctionally bonded sorbent provides the widest usable pH range (1-12), excellent low pH stability, and ultra-low column bleed.
- C8 chemistry
- Reversed phase separation
- Hybrid particle substrate
- pH range: 1 to 12
- Temperature limit: 60°C
- Max pressure: 6000 psi (415 Bar)
- Endcapped
- Low silanol activity
- Spherical particle shape
- 5 μm particle size
- 130 Å pore size
- OBD prep column format
- For HPLC preparative systems
- BEH particle technology
- Supports LC/MS and LC
- USP classification: L7
- Inner diameter: 30 mm
- Length: 50 mm
- 13% carbon load
Waters Corp Waters XBridge Preparative Column, BEH Shield RP18, 5 µm, 30 x 100 mm
This XBridge Preparative Column is an OBD type column designed for preparative chromatography. It features BEH Shield RP18 chemistry, a particle size of 5 µm, a pore diameter of 130 Å, and dimensions of 30 x 100 mm.
- OBD column type for preparative applications.
- BEH Shield RP18 chemistry provides unique selectivity.
- 5 µm particle size enables efficient separations.
- 130 Å pore diameter for optimized analyte interaction.
- Dimensions of 30 x 100 mm for specific chromatography requirements.
Waters Corp WATERS CORPORATION XBridge BEH Shield RP18 Column, 130Å, 5 μm, 3 mm x 100 mm
XBridge BEH Shield RP18 columns are designed to complement XBridge BEH C18 and C8 phases, offering a valuable tool for HPLC method development. The embedded carbamate group within the bonded phase ligand provides an alternative selectivity, particularly for phenolic compounds, when compared to traditional straight-chain alkyl columns.
- Chemistry: C18
- Separation mode: Reversed Phase
- Particle substrate: Hybrid
- pH range: 2 pH to 11 pH
- Maximum pressure: 6000 psi (415 Bar)
- Endcapped: Yes
- Bonding technology: Shield RP18
- Silanol activity: Low
- Particle shape: Spherical
- Particle size: 5 μm
- Pore size: 130 Å
- Format: Column
- Surface area: 185
- System: HPLC
- Particle technology: BEH
- USP classification: L1
- Inner diameter: 3 mm
- Length: 100 mm
- Carbon load: 17%