HPLC Columns and Cartridges
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Thermo Scientific™ Hypersil GOLD™ Silica HPLC Columns
Efficiently separate nonpolar and moderately polar organic compounds by normal phase chromatography using Thermo Scientific™ Hypersil GOLD™ Silica LC Columns.
| Pore Size | 175 Å |
|---|---|
| Stationary Phase | Silica |
| Temperature | 60°C |
| Phase | Normal Phase and HILIC |
| pH | 2 to 8 |
| Surface Area | 220 m²/g |
| USP Type | L3 |
MilliporeSigma™ Supelco™ Ascentis™ Express Phenyl-Hexyl HPLC Column, 2.7 μm Particle Size
Ascentis™ Express Phenyl-Hexyl phase columns have unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2.7 μm |
| Stationary Phase | Phenyl-Hexyl |
| Phase | Reversed Phase |
| Matrix | Phenyl-Hexyl Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
Thermo Scientific™ EASY-Spray™ HPLC Columns
Robust capillary flow HPLC columns for sensitive protein, peptide and mAbs separation using the stand alone or integrated easy to use design.
| Phase | Reversed Phase |
|---|
Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Columns
Separate challenging peptide mapping samples with Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC columns.
| Pore Size | 100 Å |
|---|---|
| Stationary Phase | C18 |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| For Use With (Application) | Bottom-up Proteomics, LC-MS |
| Surface Area | 450 m2/g |
| Max. Pressure | 22,000 psi (1500 bar) |
| Column Format | Analytical Column, EASY-Spray™ |
| Particle Size | 2 μm |
| Carbon Load | 15% |
| Packing Material | Spherical, Fully Porous, Ultrapure Silica |
| Diameter (Metric) | 75 μm |
| pH | 2 to 8 |
| Product Line | Easy Spray |
| USP Type | L1 |
Thermo Scientific™ Accucore™ Biphenyl Reversed Phase HPLC Columns
Use this solid core column for fast and reliable separation of critical pairs and isomers.
| Pore Size | 80 Å |
|---|---|
| Stationary Phase | Biphenyl |
| Temperature | 70°C |
| Phase | Reversed Phase |
| For Use With | UHPLC |
| Endcapped | Yes |
| Surface Area | 130 m²/g |
| Max. Pressure | 8700 psi (600 bar) |
| Particle Size | 2.6 μm |
| Carbon Load | 6% |
| Packing Material | Ultrapure Silica |
| Diameter (Metric) | 2.1 mm |
| pH | 2 to 8 |
| Particle Shape | Solid Core |
| Product Line | Accucore |
| USP Type | L11 |
MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-ABZ HPLC Column, 5 μm Particle Size
SUPELCOSIL™ LC-ABZ phase has a unique deactivation technology which provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar.
| Pore Size | 120 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Amide, Alkyl |
| Phase | Reversed Phase |
| Matrix | Amide, Alkyl Phase/Silica Gel, Spherical Particle Platform |
| Endcapped | Yes |
| Surface Area | 170 m2/g |
| Product Line | SUPELCOSIL |
Thermo Scientific™ BioBasic™ 8 HPLC Columns
Use this column for reversed-phase separation of proteins and other biomolecules.
| Pore Size | 300 Å |
|---|---|
| Particle Size | 5 μm |
| Carbon Load | 5% |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| pH | 2 to 9 |
| Surface Area | 100 m²/g |
| Max. Pressure | 5800 psi (400 bar) |
| Product Line | BioBasic |
| USP Type | L7 |
MilliporeSigma™ Supelco™ Ascentis™ RP-Amide HPLC Column, 3 μm Particle Size
Ascentis™ RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis™ RP-Amide is the first choice in embedded polar group HPLC phases.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | Amide, Alkyl |
| Phase | Reversed Phase |
| Matrix | Amide, Alkyl Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| pH | 2 to 8 |
| Technique | Fused-Core HPLC |
| Surface Area | 450 m2/g |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ Ascentis™ Express ES-Cyano HPLC Column, 5 μm Particle Size
The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Diisopropyl-Cyanopropylsilane |
| Phase | Reversed Phase |
| Matrix | Diisopropyl-Cyanopropylsilane Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| pH | 2 to 9 |
| Technique | Fused-Core HPLC |
| Product Line | Ascentis |
Thermo Scientific™ Hypersil GOLD™ SAX HPLC Columns
Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil™ GOLD SAX LC Columns.
| Pore Size | 175 Å |
|---|---|
| Carbon Load | 2.5% |
| Stationary Phase | SAX |
| Temperature | 60°C |
| Phase | Ion Exchange |
| pH | 2 to 8 |
| Surface Area | 220 m²/g |
| USP Type | L14 |
Thermo Scientific™ Syncronis™ Amino HPLC Columns
Maximize liquid chromatography reproducibility and laboratory productivity using Thermo Scientific™ Syncronis™ Amino LC Columns.
| Pore Size | 100 Å |
|---|---|
| Carbon Load | 4% |
| Stationary Phase | Amino (NH2) |
| Temperature | 60°C |
| Phase | Reversed Phase, Normal Phase, Ion Exchange, HILIC |
| Endcapped | Yes |
| Packing Material | Spherical, Fully Porous, Ultrapure Silica |
| Column Type | Mixed Mode |
| pH | 2 to 8 |
| Surface Area | 320 m²/g |
| Max. Pressure | 5800 psi (400 bar) |
| USP Type | L8 |
MilliporeSigma™ Chromolith™ High Resolution RP-18 Endcapped HPLC Guard Cartridges
Chromolith™ High Resolution 2 mm I.D. columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilan with full end-capping.
MilliporeSigma™ LiChrospher™ LiChroCART™ RP-18 C18 Reversed Phase HPLC Column, 5 μm, 4.6 mm x 125 mm
Column Format: Cartridge; Pore Size: 100 Å; Column Phase: Reversed Phase
MilliporeSigma™ LiChroCART™ RP-18 HPLC Cartridge, 250-4
Functions in reverse phase with RP-18 stationary phase. MilliporeSigma™ LiChroCART™ RP-18 HPLC Cartridge, 250-4 comes with customized packing (please specify packing material) and is 250mmL x 4mmI.D.
Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC Columns
Get the performance you need with ProPac™ 3R SCX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and biosimilar proteins.
| Pore Size | Nonporous |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | Proprietary |
| Temperature | Ambient to 60°C |
| Phase | Strong Cation Exchange |
| Packing Material | Monodisperse Polymer Resin |
| Column Type | Ion Exchange |
| Mobile Phase Compatibility | Recommended for use with CX-1 pH buffers |
| pH | 2 to 12 |
| Technique | Bio-LC |
| Max. Pressure | 4500 psi (310 bar) |
| Product Line | ProPac |
| Column Format | Analytical Column |