HPLC Columns
Mac-Mod Vydac 219TP Diphenyl, 300 Å, 5 µm, 2.1 x 250 mm HPLC Column
Vydac TP was the first range of wide pore media phases developed and became the industry standard for peptide and protein separations. The 300 ¿TP silica gives polypeptide molecules complete access to the interior of the silica pores. Vydac TP is known for long column lifetime and negligible phase leaching. Vydac TP provides reliable protein purifications, scalable from analytical to preparative scale. Vydac 218TP and 238TP are used for small polypeptides of 4-5kDa, enzymatic digest fragments, natural and synthetic peptides and multi-ring compounds. Vydac 208TP is recommended for polypeptides of molecular weight 10-20kDa. Vydac 214TP is recommended for the analysis of glycoproteins, hemoglobin variants, histones, insulin variants and membrane proteins. Vydac 219TP is suitable for polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides and fusion proteins from inclusion bodies.
Waters Corp Waters Anion-Exchange Column, 2.5 μm, 4.6 mm, 100 mm, Ion-Exchange
Gen-Pak FAX columns offer high resolution for anion-exchange HPLC of nucleic acids. These columns contain a weak anion exchanger based on DEAE functionalized non-porous resin with 2.5 μm spherical particles, suitable for analytical and micro-preparative applications. Measuring 4.6 mm in inner diameter and 100 mm in length, they are constructed from steel and designed for high-resolution analysis and purification of various nucleic acid species.
- High resolution for anion-exchange HPLC of nucleic acids.
- Weak anion exchanger based on DEAE functionalized non-porous resin.
- 2.5 μm spherical particles for analytical and micro-preparative use.
- Dimensions: 4.6 mm inner diameter and 100 mm length.
- Constructed from steel.
- Designed for high-resolution analysis and purification of nucleic acid species.
- pH operating range of 2 to 8.
- Maximum pressure of 6000 psi (415 bar).
Sepax Technologies Inc Sepax HPLC Column Carbomix K-NP10, 5% Crosslinkage, 10 x 300 mm
Carbomix phases are composed of spherical, low cross-linking polystyrene/divinylbenzene (PS/DVB) particles with their surfaces modified with sulfonic acid (-SO3H), followed by chelating of metal ions of calcium (Ca2+), lead (Pb2+), sodium (Na+) or potassium (K+) to form Carbomix Ca-NP, Pb-NP, Na-NP, K-NP phases, respectively. The PS/DVB support has cross-linking degree of 5%, 8% and 10% with the particle sizes of 5 and 10 µm. The uniform particle size adds the benefit of high separation efficiency to Carbomix. Carbomix resins have been specifically designed for high resolution separation of carbohydrates, organic acids, peptides, and nucleic acids.
Mac-Mod Avantor ACE C18, 100 Å, 3 µm, 2.1 x 35 mm HPLC Column
Avantor ACE Excel UHPLC and HPLC columns are manufactured with 100 Å Ultra-Inert Base Deactivated Silica specifically designed for your HPLC method development needs - Note SuperC18 is 95 Å Avantor ACE HPLC columns are available in a wide range of bonded phases (i.e. C18, C18-AR, C18-PFP, C18-Amide, CN-ES, SuperC18, NH2, C8, C4, Phenyl, AQ, CN and Sil). Avantor ACE Excel UHPLC and HPLC columns are avaialble in 1.7, 2, 3, and 5 µm particle sizes in all analytical column sizes. All Avantor ACE Excel columns are known for their excellent peak shape and batch to batch reproducibility. Columns are packed in 1000 bar UHPLC hardware. Note: Avantor ACE Excel UHPLC guard columns are available for all 1.7 and 2 µm particle sizes across all 2.1 mm - 4.6 mm column ID - Note: H0011 connects directly to the analytical column - Guard column cartridges are available in 3-packs. For 3 and 5 µm Excel columns use same guard systems used for Avantor ACE HPLC analytical columns.
Mac-Mod Princeton HA-DEA (Diethylamino) 60 Å, 1.8 µm, 4.6 x 50 mm SFC Column
Princeton Chromatography Achiral SFC columns are designed and manufactured in the US, well known for innovating novel and robust workhorse phase chemistries, for the analysis of achiral SFC applications. Princeton designs myriad phase chemistries to improve the selectivity and efficiency of SFC analysis. Known for originally innovating the 2-EP phase chemistry, explore what Princeton has to offer. Columns are available from analytical to preparative sizes and are available in a wide range of pore sizes, particle sizes, column ID's and lengths. If you do not see something that you want, please just ask!
Mac-Mod Evosphere PFP, 100 Å, 5 µm, 0.5 x 150 mm Monodisperse Fully Porous Particle HPLC Column
Evosphere UHPLC and HPLC columns are 100 A Monodispersed Fully Porous Silica-based columns designed to combine the benefits of Fully Porous and Core-Shell particle technology into an improved particle design and morphology that brings cutting-edge loading capacity to ultra-high-resolution particle columns. The Monodispersed Particles provide similar efficiency to Core-Shell products when comparing them directly via particle size matching and significantly increase loadability which allows ultra-high resolution analytical separations to scale to preparative separations for isolation work if needed. The bonded phase chemistries are exciting as well to maximize selectivity with novel C12 bonded phase, 2 multi-modal C18/AR, and C18/PFP bonded phases, an extended and stabilized phenyl funtionality in the Diphenyl bonded phase, a polar endcapped AQUA phase, a Phenyl-Hexyl phase, a propyl PFP phase and the RP18-Amide bonded phases which is great for doing bases at low pH.
Advanced Materials Technology HALO 160 Å PCS C18, 2 µm, 2.1 mm x 100 mm
Built upon proven Fused-Core technology for speed and efficiency, the HALO PCS column products are positively charged surface chemistries designed to deliver improved peak shapes for basic compounds observed with standard C18 chemistry. Ideal for use with low ionic strength mobile phases, HALO PCS maintains peak symmetry at higher loading capacities and provides alternate selectivities from C18. Available in both a 90 Å and 160 Å pore size for small molecule and peptide analysis. The columns are optimized to deliver performance for reproducible, high efficiency LC and LCMS separations.
Waters Corp Waters Column, C18, 5 μm, 3 mm, 100 mm
The XBridge BEH C18 sorbent serves as a workhorse for HPLC method development and is suitable for use across a mobile phase pH range of 1 to 12. Its trifunctionally bonded C18 ligand offers a universal column for most HPLC separations, providing the widest usable pH range, superior low pH stability, and ultra-low column bleed.
- Utilizes patented trifunctional bonding and end-capping technology to prevent low-pH instability caused by siloxane bond cleavage via acid hydrolysis.
- Ethylene bridges within the silica matrix address issues associated with silica-based chromatographic particle failure at high pH, such as particle dissolving, column voids, efficiency loss, and peak degradation.
- Produced in cGMP, ISO9001, and ISO13485 certified facilities, ensuring exceptional reproducibility and consistent column performance.
Mac-Mod Avantor Alltima C8, 100 Å, 3 µm, 4.6 x 50 mm HPLC Column
vantor Alltima columns combines a range of different phase chemistries with base deactivated silica. Avantor Alltima columns provides stable bonding for long column lifetimes. Avantor Alltima columns are made of high purity silica that provide excellent column stability with multiple selectivity options.
Sepax Technologies Inc Sepax HPLC Column GP-C18, 5 µm, 120 Å, 7.8 x 250 mm
GP-C18 uses full coverage bonded silica packing, which provides exceptionally high stability. The unique mono-functional bonding chemistry of GP-C18 avoids the formation of multiple C18 layers. Such uniform stationary phase allows the separation to achieve high selectivity and high efficiency.
Mac-Mod Avantor Alltima C18, 100 Å, 5 µm, 4.6 x 250 mm HPLC Column-W (Waters Port)
vantor Alltima columns combines a range of different phase chemistries with base deactivated silica. Avantor Alltima columns provides stable bonding for long column lifetimes. Avantor Alltima columns are made of high purity silica that provide excellent column stability with multiple selectivity options.