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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Length (Metric)

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Phase

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211 – 225 of 17,666 results
211

MilliporeSigma™ Supelco™ apHera™ C18 HPLC Column, 5 μm Particle Size

apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either.

Pore Size 300 Å
Particle Size 5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Product Line apHera
212

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

213

MilliporeSigma™ Supelco™ Ascentis™ Express C18 Micron Capillary HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
214

MilliporeSigma™ Supelco™ SUPELCOSIL™ ABZ+Plus HPLC Column, 5 μm Particle Size

Offer both high deactivation and unique selectivity.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
215

MilliporeSigma™ Supelco™ Ascentis™ Express F5 UHPLC Column, 2 μm Particle Size

Ascentis™ Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Pore Size 90 Å
Particle Size 2 μm
Type LC-MS Column
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase
Matrix PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core UHPLC
Product Line Ascentis
216

MilliporeSigma™ Supelco™ BIOshell™ A160 Peptide C18 Column, 2 μm Particle Size

Shell Thickness: 0.4 μm. Solid Core: 1.2 μm. Extent of labeling: 4.0%

Pore Size 160 Å
Particle Size 2 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Spherical Silica Particle Platform
Endcapped No
Material Stainless Steel Hardware
Technique Fused-Core HPLC
Surface Area 65 m2/g
Product Line BIOshell
217

MilliporeSigma™ Supelco™ Discovery™ Cyano HPLC Column, 5 μm Particle Size

Discovery™ Cyano HPLC column is used for the retention and separation of strongly basic analytes including quaternary ammonium salts, with excellent peak shapes.

Pore Size 180 Å
Particle Size 5 μm
Stationary Phase Cyano
Phase Reversed Phase, Normal Phase, HILIC
Matrix Cyano Phase/Silica Gel, High Purity, Spherical Base Material
Endcapped Yes
pH 2 to 8
Surface Area 200 m2/g
Product Line Discovery
218

MilliporeSigma™ Supelco™ Kromasil C18 HPLC Column, 100 Å, 3.5 μm Particle Size

For small molecules and peptides.

Pore Size 100 Å
Particle Size 3.5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Product Line Kromasil
219

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 10 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pore Size 300 Å
Particle Size 10 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/silica particle platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
220

MilliporeSigma™ Supelco™ SUPELCOGEL™ Pb, 6% Crosslinked HPLC Column, 9 μm Particle Size

SUPELCOGEL™ Pb, 6% crosslinked HPLC column may be used for the separation and determination of d-mannose in human serum samples using liquid chromatography coupled with tandem mass spectrometry.

Particle Size 9 μm
Stationary Phase Sulfonated Polystyrene
Phase Ion Exclusion
Matrix Sulfonated Polystyrene/Divinylbenzene Phase/Spherical Particle Platform
Diameter (Metric) 7.8 mm
Crosslinking 0.06
Technique Crosslinked HPLC
Product Line SUPELCOGEL
221

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-Diol HPLC Column, 5 μm Particle Size

LC-Diol columns can be used to separate proteins by gel filtration chromatography.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase Diol
Phase Reversed Phase
Matrix Diol Phase/Silica Gel, Spherical Particle Platform
Length (Metric) 25 cm
Endcapped No
Surface Area 170 m2/g
Product Line SUPELCOSIL
222

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-NH2 HPLC Column, 3 μm Particle Size

The amino column is most often employed for the separation of mono- and disaccharides.

Pore Size 120 Å
Particle Size 3 μm
Stationary Phase Amino (NH2)
Phase Reversed Phase
Matrix Amino (NH2) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
223

MilliporeSigma™ Supelco™ SUPELCOSIL™ ABZ+Plus HPLC Column, 3 μm Particle Size

Offer both high deactivation and unique selectivity.

Pore Size 120 Å
Particle Size 3 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
224

MilliporeSigma™ Supelco™ BIOshell™ A160 Peptide C18 HPLC Column, 160 Å, 2.7 μm Particle Size

BIOshell™ 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins.

Pore Size 160 Å
Particle Size 2.7 μm
Carbon Load 4.6%
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped No
pH 1 to 8
Material Stainless Steel Hardware
Technique Fused-Core HPLC
Surface Area 90 m2/g
Product Line BIOshell