Protein Method Blocking Buffers
Biotium Permeabilization and Blocking Buffer (5X)
Concentrated buffer stock solution (5X) for one step permeabilization and blocking for immunofluorescence staining. Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer for use with anti-goat and anti-sheep secondary antibodies.
LICORbio Intercept T20 (TBS) Protein-Free Antibody Diluent (500 mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Sigma Aldrich Fine Chemicals Biosciences Carbonate-Bicarbonate Buffer capsule | 50CAP
Carbonate-Bicarbonate Buffer capsule | 50CAP
Electron Microscopy Sciences Aurion Blocking Solution Donkey 30 ML
Aurion has developed Blocking Solutions which effectively block ‘sticky' surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.
Carbosynth LLC. HAMA blocking reagent, 1 gram
HAMA blocking reagent for use in assays specific for clinical false positive samples
Cell Signaling Technology Animal-Free Blocking Solution, 5X, 250 mL
Animal-Free Blocking Solution (5X) is a plant-based reagent developed to minimize background in chromogenic immunohistochemical assays and western blotting. This formulation is designed to replace traditional blocking agents such as normal goat serum, BSA, or non-fat dry milk, providing a solution free of animal-derived proteins.
- Contains no animal-derived proteins
- Formulated for use in western blotting and immunohistochemistry
- Concentrated 5X formulation requiring dilution to 1X with deionized water prior to use
- Stable for 36 months when stored at +4°C and protected from light
LICORbio Intercept (PBS) Protein-Free Blocking Buffer (3x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
LICORbio Intercept (PBS) Protein-Free Blocking Buffer (10x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Abcam Blocking Buffer 10X, 50ML
Blocking Buffer 10X (ab210904) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
The product is subject to the following: Abcam Restricted Use Statement
LICORbio Intercept (TBS) Blocking Buffer and Diluent Kit
Try Intercept (TBS) Blocking Buffer and Intercept T20 (TBS) Antibody Diluent for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability. Intercept T20 Antibody Diluents improve the specificity of the primary and secondary antibodies, reducing off-target effects. They are preformulated with Tween 20. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation. This kit includes Intercept (TBS) Blocking Buffer (1 x 500 mL) and Intercept T20 (TBS) Antibody Diluent (2 x 500 mL)
Biolegend Tris-Buffered Saline 20X (1.0 M) w/ Tween 20
Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 Apps: IHC; Size: 500 mL
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting - MB-070S
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Leica Biosystems PEROXIDASE BLOCK NOVOCASTRA
Novocastra Peroxidase Block, RE7101, is intended for use in the peroxidase based immunohistochemical (IHC) staining procedures. The presence of pseudoperoxidase (erythrocytes) and endogenous peroxidase in paraffin sections to be stained by immunoperoxidase procedures, can result in nonspecific staining. A method for the blocking of pseudoperoxidase was described (StreefkerkJG, Journal of Histochemistry and Cytochemistry. 20: 829 (1972)). This product is used in a peroxidase based IHC procedure. Incubating sections with Novocastra Peroxidase Block, RE7101, can neutralize endogenous peroxidase activity. 25 mL of reagent is supplied.