Protein Method Blocking Buffers
Sigma Aldrich Fine Chemicals Biosciences OmniPur(R) Nonfat Powdered Milk | 10GM
OmniPur(R) Nonfat Powdered Milk | 10GM
Clontech Labs 3P Wash and Stop Solution for ELISA without Sulfuric Acid | 5 x 96 Rxns
This product contains 10X PBS and Tween20 for preparing the wash solution and Stop Solution for stopping the peroxidase reaction, which are used together with TaKaRa Bio' s ELISA Kits. Although the Stop Solution is not sulfuric acid (H2SO4) -based, it can be used like 1N H2SO4. Use the prepared wash solution and Stop Solution according to the user manual for each ELISA Kit.
Alpha Diagnostic International ELISA PLATE BLOCKING BUFFER CO
ELISA Plate Blocking Buffer concentrate (10X) BSA-based
Teknova 1X PBS Solution with 0.05% Tween-20 (pH 7.4). 20L Carboy, Sterile
137mM Sodium Chloride, 2.7mM Potassium Chloride,4.3mM Sodium Phosphate, 1.4mM Potassium Phosphate,0.05% Tween (v/v)
Santa Cruz Biotechnology Tris Buffered Saline with Tween 20: 1 L of 20X, 1 L
Tris Buffered Saline with Tween 20: 1 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Surmodics Inc StabilBlock Immunoassay Stabilizer 50ml
StabilBlock™ Immunoassay Stabilizer effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefits are reduction of non-specific binding while increasing signal-to-noise ratios within immunoassay applications.
Nacalai USA Inc BLOCKING ONE, 500ML
Blocking One is superior to conventional blocking solutions. The preservative does not affect the enzyme activity of POD or ALP. Only simple refrigerator storage is necessary, even after opening the bottle.
Boca Scientific Inc The Blocking Solution, 50ml
The Blocking Solution is a high-performance blocking reagent designed for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces in immunoassays. It is ideal for assays that require high reproducibility and low coefficients of variation, such as assays for pre-clinical and clinical studies, production of high quality diagnostics or measurement of precious specimens. The Blocking Solution is based on highly purified casein that has been chemically modified for maximum adsorption efficiency and a dense blocking layer. By incubating a surface with The Blocking Solution, such as a microtiter plate or western blotting membrane, free spaces on the surface are saturated by the components of this blocker. This minimizes unwanted binding of the analyte or the detection antibody to the surface, avoiding background and improving sensitivity of the assay.
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (10X, with 1% Tween-20, pH 7.4) - 1L
10X Phosphate Buffered Saline with Tween-20 (PBST) is a specialized variant of PBS, supplemented with Tween-20, a non-ionic surfactant. Tween-20 minimizes the non-specific binding and background noise, resuting in enhanced signal to noise ratio. PBST is often used in immunoassays such as ELISA and Western Blotting, where it serves as a washing and blocking buffer. With a stable buffering pH range between 7.0 and 7.4, PBST maintains ionic strength and protein solubility for biological assays.
Electron Microscopy Sciences Aurion R-Gent SE-EM Kit 30 ML
AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected and is compatible with heavy metal counterstaining. The reagent has extremely delayed auto-nucleation and can be used under standard laboratory light conditions. It also has low viscosity, which is especially advantageous for pre-embedding immunogold applications.
Alkali Scientific CellPro™ PBS 20X + Tween 20, 1 L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Quality Biological Inc DPBS with TWEEN 20 without Ca & Mg, 1000 mL
DPBS can be used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6. ¿ DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ DPBS is also used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate, to wash the wells of microtiter plates, and to wash cells during preparation and serial transfer. ¿ 1X working solution is recommended ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months