Protein Method Blocking Buffers
Teknova 0.01M PBS with 0.02% Tween-80. 1000mL, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Active Motif MAXblock™ Blocking Medium
MAXblock Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.
Seracare Life Sciences Inc 10X TBS W/ 10% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 10% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. This formulation of TBST has a higher concentration of Tween 20 and provides a more stringent wash than standard TBST formulations.
Seracare Life Sciences Inc 10X TBS W/ 0 5% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 0.5% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures.
Research Products International Corp TBS with Tween, 20X, 7.4 pH, Sterile Buffered, 1 Liter
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is a commonly used buffer solution in the life science marketplace. TBS stands for Tris-Buffered Saline, and Tween refers to Tween 20, a nonionic detergent.
TBS with Tween is primarily used in various applications such as Western blotting, immunohistochemistry, and antibody-based assays. It serves as a buffer for sample dilution, antibody washing, and blocking steps during these experimental procedures.
The buffer solution is prepared at a 20X concentration, meaning it is twenty times more concentrated than the final desired concentration. This concentrated form allows for efficient storage and transportation.
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is specifically formulated to have a pH of 7.4, which is the physiological pH of many biological systems.
LAB BIOREAGENTS StrongBlock III | 500mL
Lab Bioreagents Buffers have been developed through the screening of >2000 compounds with the support of machine learning (AI) and optimized through kinetic and dynamic binding tests. StrongBlock (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlock (I, II, III) are a variety of buffers designed by different blocker components.
Biocare Medical, LLC Mouse-on-Mouse HRP-Polymer Bundle - 6.0 ml
The Mouse-on-Mouse HRP-Polymer is specially designed for using mouse primary antibodies on mouse tissues with minimum cross-reactivity to endogenous mouse IgG. Utilization of this goat anti-mouse horseradish peroxidase (HRP) polymer is advantageous over traditional biotinylation methods. Biotinylated anti-mouse secondary antibodies can bind to endogenous mouse IgG in the tissue in addition to the primary antibody, leading to troublesome background staining. Biocare Medical's biotin-free polymer technology provides the elimination of endogenous biotin, reduction of IHC steps and increased sensitivity.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Nacalai USA Inc BLOCKING ONE, 500ML
Blocking One is superior to conventional blocking solutions. The preservative does not affect the enzyme activity of POD or ALP. Only simple refrigerator storage is necessary, even after opening the bottle.
Seracare Life Sciences Inc 10X PBS W/ TWEEN 20 (PBST) 1L
10X Phosphate-buffered Saline with Tween 20 (PBST) is ideal for use as a wash buffer in general immunoassay procedures. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.