Protein Method Blocking Buffers
Kd Medical Inc PBS 20X PLUS TWEEN 20 4L
Phosphate Buffered Saline and Tween 20 in a 20X concentrate for use as a wash buffer in immuno-labeling techniques. One 4 liter cubitainer.
Kd Medical Inc PBS 20X + TWEEN 20, 1 L
Concentrated 20X PBS and Tween 20 used to be diluted to a 1X working solution. Used as a wash buffer in various laboratory applications. One 1 Liter bottle.
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Alkali Scientific CellPro™ DPBS, 1X, with Tween 20, 1L, 12/cs
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Electron Microscopy Sciences Aurion Tween-20 10 ML
Tween-20 is a non-ionic detergent with a molecular weight of about 600 and a critical micelle concentration (CMC) of 0.06-0.07% in water at room temperature. Its working mechanism may in part be based on its action as a detergent, binding to the hydrophobic moieties of water insoluble compounds, rendering them hydrophilic. In addition, immuno-compounds may become incorporated into micelles when the Tween-20 concentration is higher than the CMC, for instance at 0.1 % in PBS at pH 7.4.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting - MB-070
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Crescent Chemical Co Inc Denhardt's Solution, 50X Concentration, 10mL
Denhardt's Solution, 50X Concentration, 10mL, Molecular Biology Grade, DNase/RNase not detected.Suitable for nucleic acid hybridization. Denhardt's solution is a mixture of blocking agents capable of saturating non-specific binding sites and to be used in membrane-based hybridization protocols. It is recommended for use with nylon membranes. Storage Conditions: -15 °C TO -25 °C
Boca Scientific Inc SmartBlock™, 500ml
SmartBlock™ is a BSA-free, peptide-based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces, removing background and preventing unwanted binding. This reagent was produced by chemically modifying peptides in a specific process that resulted in a peptide solution with strong blocking characteristics. SmartBlock™ has high lot-to-lot consistency that had previously only been obtained by synthetic blockers. For many assays, SmartBlock™ is an economical and reliable alternative to BSA-containing solutions. Problems caused by BSA within blockers, such as BSA-interactions and cross reactivities, can be eliminated by using SmartBlock™.
Vector Laboratories Animal-Free Blocker and Diluent (AFB), Ready-to-Use (R.T.U.) 100 ml
R.T.U. Animal-Free Blocker and Diluent is a ready-to-use antibody diluent and blocking reagent that stabilizes diluted antibody solutions and diminishes background due to non-specific binding in immunohistochemical applications. It contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations where these or other traditional blocking agents are ineffective. It is especially convenient for multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
Nacalai USA Inc BLOCKING ONE, 500ML
Blocking One is superior to conventional blocking solutions. The preservative does not affect the enzyme activity of POD or ALP. Only simple refrigerator storage is necessary, even after opening the bottle.
Research Products International Corp Casein Blocking Solution in TBS, 1 Liter
Contains 3% Casein in TBS buffer. Optimized for use in western blotting to minimize non-specific binding of antibodies.
Sigma Aldrich Fine Chemicals Biosciences Gelatin blocking buffer for Western blotting, powder blend | 1L
Gelatin blocking buffer for Western blotting, powder blend | 1L
Abcam Immunoassay Blocking Buffer, 1000ML
Abcam’s Immunoassay Blocking Buffer (ab171534) effectively preserves the conformation and activity of dried proteins in immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
Seracare Life Sciences Inc 10X PBS W/ TWEEN 20 (PBST) 1L
10X Phosphate-buffered Saline with Tween 20 (PBST) is ideal for use as a wash buffer in general immunoassay procedures. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.