Protein Method Blocking Buffers
Thermo Scientific Chemicals BSA Blocking Buffer, 5% in PBS, with 0.05% Tween-20
BSA (bovine serum albumin) blocking buffer, 3% in PBS, is a blocking agent ideal for saturating excess protein-binding sites on membranes.
MilliporeSigma™ HAT Assay Buffer, 5X
For use in the HAT Assay.
MilliporeSigma™ Chemicon™ Choline Acetyltransferase Blocking Peptide
For Blocking AB143, AB144P Antibodies
Pharmingen™ Purified NA/LE Human BD Fc Block™
0.25mg in a 1.0mg/mL concentration
Azure Biosystems Fluorescent Blot Blocking Buffer
Blocking solution for fluorescent Western blot, 1X solution, 500 mL
TBST (Tris-Buffered Saline + Tween 20), 20X Solution, Molecular Biology Grade, Hoefer™
Dilute to 1X with high purity H20 for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween™ 20
LI COR BIOTECH LLC Intercept (TBS) Blocking Buffer (500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.
Bio Rad Laboratories EveryBlot Blocking Buffer, 500 mL
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
LI COR BIOTECH LLC Intercept (TBS) Blocking Buffer (3 x 500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.