Protein Method Blocking Buffers
Thermo Scientific Chemicals BSA Blocking Buffer, 3% in PBS
BSA (bovine serum albumin) blocking buffer, 3% in PBS, is a blocking agent ideal for saturating excess protein-binding sites on membranes.
MilliporeSigma™ Calbiochem™ BLOT-QuickBlocker™ Reagent
Prevents nonspecific proteins from binding to blots.
MilliporeSigma™ HAT Assay Buffer, 5X
For use in the HAT Assay.
MilliporeSigma™ Chemicon™ Choline Acetyltransferase Blocking Peptide
For Blocking AB143, AB144P Antibodies
Azure Biosystems Chemi Blot Blocking Buffer
Blocking solution for chemiluminescent Western blot, 1X Solution
Azure Biosystems Protein-Free Blot Blocking Buffer
Protein-free blocking solution for Western blot, 5X concentrate
Azure Biosystems Fluorescent Blot Blocking Buffer
Blocking solution for fluorescent Western blot, 1X solution, 500 mL
TBST (Tris-Buffered Saline + Tween 20), 20X Solution, Molecular Biology Grade, Hoefer™
Dilute to 1X with high purity H20 for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween™ 20
Active Motif MAXblock™ Blocking Medium
MAXblock Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.
Abcam 10 x Blocking Buffer, 50ML
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
The product is subject to the following: Abcam Restricted Use Statement
LICOR Intercept (TBS) Protein-Free Blocking Buffer (500 mL)
Item is non returnable
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection.