Protein Method Blocking Buffers
Thermo Scientific Chemicals Casein Blocking Buffer, 3% in PBS
For Western blots and ELISA
MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets
Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C
MilliporeSigma™ HAT Assay Buffer, 5X
For use in the HAT Assay.
BD OptEIA™ Assay Diluent
A buffered protein solution containing fetal bovine serum that is intended for use in BD OptEIA ELISA Sets
MilliporeSigma™ Chemicon™ Choline Acetyltransferase Blocking Peptide
For Blocking AB143, AB144P Antibodies
Azure Biosystems Protein-Free Blot Blocking Buffer
Protein-free blocking solution for Western blot, 5X concentrate
Azure Biosystems Fluorescent Blot Blocking Buffer
Blocking solution for fluorescent Western blot, 1X solution, 500 mL
TBST (Tris-Buffered Saline + Tween 20), 20X Solution, Molecular Biology Grade, Hoefer™
Dilute to 1X with high purity H20 for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween™ 20
Active Motif MAXblock™ Blocking Medium
MAXblock Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.
LICOR Intercept (TBS) Protein-Free Blocking Buffer (500 mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
LICOR Intercept (TBS) Blocking Buffer (500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.