Protein Method Blocking Buffers
MilliporeSigma™ HAT Assay Buffer, 5X
For use in the HAT Assay.
MilliporeSigma™ Calbiochem™ BLOT-QuickBlocker™ Reagent
Prevents nonspecific proteins from binding to blots.
MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets
Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C
Thermo Scientific Chemicals Casein Blocking Buffer, 3% in PBS
For Western blots and ELISA
Azure Biosystems Protein-Free Blot Blocking Buffer
Protein-free blocking solution for Western blot, 5X concentrate
Azure Biosystems Fluorescent Blot Blocking Buffer
Blocking solution for fluorescent Western blot, 1X solution, 500 mL
Azure Biosystems Chemi Blot Blocking Buffer
Blocking solution for chemiluminescent Western blot, 1X Solution
TBST (Tris-Buffered Saline + Tween 20), 20X Solution, Molecular Biology Grade, Hoefer™
Dilute to 1X with high purity H20 for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween™ 20
Active Motif MAXblock™ Blocking Medium
MAXblock Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.
Abcam 10 x Blocking Buffer, 50ML
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
The product is subject to the following: Abcam Restricted Use Statement
