Protein Method Blocking Buffers
MilliporeSigma™ HAT Assay Buffer, 5X
For use in the HAT Assay.
Epredia™ Lab Vision™ Phosphate Buffered Saline and Tween™ 20 (20X)
Employ a wash buffer for immunolabeling techniques with the Epredia™ Lab Vision™ Phosphate Buffered Saline and Tween™ 20 (20X).
BLOTTO antifoam in PBS, with 0.02% sodium azide, Thermo Scientific™
Blocking reagent for immunohistochemistry experiments
MilliporeSigma™ Calbiochem™ PBS-TWEEN™ Tablets
Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN™ 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C
BLOTTO and Tween 20 in PBS, with 0.02% sodium azide, Thermo Scientific™
Blocking reagent for immunohistochemistry experiments
Azure Biosystems Fluorescent Blot Blocking Buffer
Blocking solution for fluorescent Western blot, 1X solution, 500 mL
Hoefer, Inc TBST (Tris-Buffered Saline + Tween 20), 20X Solution, Molecular Biology Grade, Hoefer™
Dilute to 1X with high purity H20 for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween™ 20
LI COR BIOTECH LLC Intercept (TBS) Blocking Buffer (500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.
Bio Rad Laboratories EveryBlot Blocking Buffer, 500 mL
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
Surmodics Inc StabilBlock Immunoassay Stabilizer, 1L
StabilBlock Immunoassay Stabilizer effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefits are reduction of non-specific binding while increasing signal-to-noise ratios within immunoassay applications.
LI COR BIOTECH LLC Intercept (TBS) Blocking Buffer (3 x 500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.