Gel Electrophoresis Buffers and Diluents
Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Invitrogen™ Bolt™ Transfer Buffer (20X)
Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting
Invitrogen™ NativePAGE™ Sample Buffer (4X)
A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System
Invitrogen™ Fluorescent Compatible Sample Buffer (4X, non-reducing)
Invitrogen Fluorescent Compatible Sample Buffer is for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Thermo Scientific™ Pierce™ 10X Western Blot Transfer Buffer, Methanol-free
A methanol-free concentrated solution to easily and quickly prepare MeOH-free Western blot transfer buffer for tank-based or other wet gel-to-membrane transfer methods.
Invitrogen™ NuPAGE™ MES SDS Running Buffer (Powder)
Invitrogen NuPAGE MES SDS Running Buffer (Powder) is formulated for use with Bis-Tris gels to deliver fast SDS-PAGE separations of small- to medium-sized proteins. Supplied as a powder for flexible preparation, room temperature storage, and a long 2-year shelf life.
Invitrogen™ NativePAGE™ Running Buffer (20X)
Used to make the NativePAGE Cathode and Anode Running Buffers
Invitrogen™ NativePAGE™ 5% G-250 Sample Additive
Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels
Invitrogen™ Novex™ IEF Cathode Buffer pH 3-7 (10X)
Optimized pre-mixed IEF buffers reduce variability and ensure consistent results