Gel Electrophoresis Buffers and Diluents
Electron Microscopy Sciences R-Universal Epitope Recovery Buffer (10x stock) 125 ML
EMS UNIVERSAL Epitope Recovery Buffer is a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups. In extensive testing the buffer has shown excellent results when used with different antibodies, including those that require low or high pH buffers or protolithic treatment for successful staining.
The EMS Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in our Antigen Retriever (62700 Series). Using our Universal Buffer in our Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections. Shelf Life: 12 months.
Boston Bioproducts Inc Citrate Buffer (0.5 M, pH 3.0) - 250ml
The Citrate Buffer comprising of sodium citrate dihydrate and citric acid is commonly used to maintain pH levels within the range of 3.0 to 6.2. This buffer supports several applications in molecular biology, pathology, histology, immunohistochemistry, and RNA isolation. It also aids in the detection of antigens in fixed tissue preparations as it induces the breaking cross-links between antigens and components in the fixation medium.
Bioworld Citrate Buffer, 0.5M, pH 5.0, 100 mL
Product DescriptionCitrate buffer solution has a 0.5M concentration at a pH of 5.0. Citrate is used as an anticoagulant, a biological buffer, and is often prepared for antigen retrieval of tissue samples. The solution is designed to break protein cross-links, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhanced stain intensity of antibodies. Citrate has as a calcium chelator that forms complexes to disrupt the tendency of blood to clot.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
New England Biolabs, Inc. T4 DNA Ligase Reaction Buffer – 5 ml
T4 DNA Ligase is the industry standard for performance and quality. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Electron Microscopy Sciences Acetate Buffer, 0.2M, pH 3.6-5.6 500 ML
Acetic Acid and Sodium Acetate, 0.2M, pH range 3.6-5.6. Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 3.6-5.6
Biotang Inc RNAse Free Sodium Acetate, 1M, pH 4.5, 100 ml
RNAse Free Sodium Acetate, 1M, pH 4.5, 100 ml.
Biotang Inc RNAse Free Sodium Acetate, 3M, pH 5.2, 100 ml
RNAse Free Sodium Acetate, 3M, pH 5.2, 100 ml.
Zymo Research Corporation RNA Pre-Wash Buffer, 20 ml
RNA Pre-Wash Buffer (25 ml). Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
As One International Inc Loading Buffer Red, 5 x 1 mL
5x DNA loading buffer used for loading DNA samples onto agarose or polyacrylamide gels and tracking migration during electrophoresis. Red tracking dye migrates at 300-500 bp
Alkali Scientific CellPro™ SSPE, 20X, pH 7.4 (Sodium Phosphate EDTA), 1L, 12/cs
CellPro™ SSPE Buffer 20X stands as a reliable reagent in molecular biology research. Crafted to stringent standards of purity and sterility, this buffer offers researchers a robust solution for nucleic acid hybridization experiments. With its sterile filtered formulation, CellPro™ SSPE Buffer ensures consistent and reproducible results, empowering researchers in their molecular biology endeavors.
Zymo Research Corporation RNA Pre-Wash Buffer, 100 ml
RNA Pre-Wash Buffer (100 ml). Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
New England Biolabs, Inc. Gel Loading Dye Purple (6X) – 4 ml
Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands. It is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes. Attention SYBR Safe and GelRed dye users: Due to an increased concentration of SDS in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.
Cayman Chemical Methyltrnsferase asy Buffe 1ea
An ago-PAM of GLP-1R induces calcium mobilization in 9-3-H cells expressing human GLP-1R (EC50 1.3 UM) enhances GLP-1- exenatide- or liraglutide-induced calcium mobilization in the same cells (EC50s 1.8 8.4 and 30 UM respectively) potentiates exenatide-induced insulin secretion in primary mouse pancreatic islets at 30 UM reverses haloperidol-induced catalepsy in a rat model of Parkinson’s disease motor symptoms at 30 mg/kg