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Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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1 – 15 of 143 results
1

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
GSA_VA

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

EDGE
Substrate Horseradish Peroxidase
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form Liquid
Product Type Substrate
Signal Duration 1 to 2 hr.
Product Line Pierce
Detection Method Chemiluminescence
2

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
GSA_VA

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.

EDGE
Content And Storage 2°C to 8°C
Sensitivity ∼10 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Western Blot
3

Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

EDGE
Content And Storage Upon receipt store at 4°C. Product shipped on ice.
Sensitivity Low picogram to mid-femtogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce
Detection Method Chemiluminescence, Fluorescence
4
Promotions Available

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
GSA_VA

Recommended HRP substrate for fluorescence imagers

EDGE
Content And Storage 2°C to 8°C
Kit Contents Detection reagent 1 and 2
Product Type ECL Western Blot Substrate
5

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
GSA_VA GREENER_CHOICE

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

EDGE
Sensitivity ∼1 pg
Product Type ECL Western Blot Detection Reagent
For Use With (Application) Sensitive Quantitative or Confirmatory Western Blot
6
Promotions Available

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
GSA_VA

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

EDGE
7

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
GSA_VA

Provides high sensitivity over a broad detection range at an economical price

EDGE
Substrate Horseradish Peroxidase
Content And Storage 2°C to 8°C
Shelf Life 9 Months
For Use With (Application) Dot/Slot Blot
8

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

9

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Contains SuperSignal™ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.

10

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

11

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

12

MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates
GSA_VA

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Product Type HRP Substrate
For Use With (Application) Dot/Slot Blot
13
Promotions Available

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
GSA_VA

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

EDGE
Shipping Condition Room Temperature
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Conjugate HRP
Form Liquid
Product Type Substrate
Signal Duration 6 to 24 hr.
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence
14

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

EDGE
15

Azure Biosystems Radiance ECL

General HRP substrate for chemiluminescent Western blots (500 mL kit).