Western Blot Substrates and Substrate Kits
Western Blot Substrates and Substrate Kits
Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
Promotions are available
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Promotions are available
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
Provides high sensitivity over a broad detection range at an economical price

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis
Promotions are available
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution
Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.
Thermo Scientific™ NBT Substrate Powder (nitro-blue tetrazolium chloride)
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
Promotions are available
Thermo Scientific™ Pierce™ DAB Substrate Kit
Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.
Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS
Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.
Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates
Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns