ELISA Substrates and Reagents
ABclonal Technology Phospho-Filamin A-S2152 Rabbit pAb
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
ABclonal Technology Phospho-SRC Family-Y416 Rabbit pAb
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
ABclonal Technology Phospho-ALOX5-S271 Rabbit pAb
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ABclonal Technology Phospho-CDK1-T161 Rabbit pAb
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ABclonal Technology Phospho-NF-kB p65/RelA-S468 Rabbit pAb
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Vector Laboratories TMB Substrate (Super Sensitive) - LS-M36, 100ml
TMB Substrate (Super Sensitive) is a ready-to-use TMB substrate for microwell applications containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. This substrate is our most sensitive TMB buffer. This sensitive, faster kinetic TMB substrate is approximately 40-fold more sensitive than our most popular substrate, TMB Substrate (ELISA).
American Research Products Inc Rat Heat shock protein HSP 90
Rat Heat shock protein HSP 90-alpha (Hsp90aa1/Hsp86/Hspca) ELISA kit from CUSABIO Detection Range 0 312 ng/mL-20 ng/mL Sensitivity 0 078 ng/mL Sample serum plasma tissue homogenates Method Sandwich
Surmodics Inc TMB One Component HRP Microwell Substrate, 1mL
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
United States Biological Corporation P-NTRPHNYL PHOSP (PNPP), 250ML
ImmunoTek™ p-Nitrophenyl Phosphate (pNPP), 250ml
Neogen Diagnostics TMB Membrane Substrate, 100 ML, HRP, Membrane, Colorimetric
TMB Membrane Substrate is a ready to use solution. It contains 3,3’,5,5’-tetramethyl-benzidine in a mildly acidic buffer in a one bottle format. TMB Membrane Substrate develops an insoluble, permanent dark blue reaction in the presence of peroxidase. The substrate is intended for western blot, dot blots, and line assays and offers comparable performance and sensitivity to some chemiluminescent substrates. This substrate is not recommended for microwell or immunohistochemical applications. Stable for 2 years, from the date of manufacture, when stored at room temperature (15 to 25C) or 48 months when stored at 2-8C.
ABclonal Technology Phospho-EPHA2-S897 Rabbit pAb
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.