Gel Electrophoresis Reagents and Buffers
ABclonal Technology BCR Rabbit pAb
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The unregulated tyrosine kinase activity of BCR-ABL1 contributes to the immortality of leukaemic cells. The BCR protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac and other kinases. Two transcript variants encoding different isoforms have been found for this gene.
ABclonal Technology SNX18 Rabbit pAb
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a SH3 domain. Multiple transcript variants encoding different isoforms have been found for this gene.
New England Biolabs, Inc. NEBuffer™ 4 – 5 ml
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1 , NEBuffer 3.1 and CutSmart Buffer (NEB #B7204) are available. Visit www.neb.com for details.
Boston Bioproducts Inc Laemmli SDS-Sample Buffer (4X, Non-Reducing) - 25ml
The 4X non-reducing laemmli sample buffer is a critical component in the preparation of protein samples for SDS-PAGE, ensuring resolution of protein bands. Its composition typically includes Tris-HCl, glycerol, SDS, and a tracking dye like Bromophenol Blue, which provide a suitable environment for protein electrophoresis. Non-reducing Laemmli Sample Buffers do not contain reducing agents, preserving disulfide bonds in proteins.
Biotang Inc Laemmli (SDS-Sample) Buffer (Non-Reducing, 6X), 25 ml
Laemmli (SDS-Sample) Buffer (Non-Reducing, 6X), 25 ml.
Cayman Chemical Methyltrnsferase asy Buffe 1ea
An ago-PAM of GLP-1R induces calcium mobilization in 9-3-H cells expressing human GLP-1R (EC50 1.3 UM) enhances GLP-1- exenatide- or liraglutide-induced calcium mobilization in the same cells (EC50s 1.8 8.4 and 30 UM respectively) potentiates exenatide-induced insulin secretion in primary mouse pancreatic islets at 30 UM reverses haloperidol-induced catalepsy in a rat model of Parkinson’s disease motor symptoms at 30 mg/kg
Dojindo Molecular Technologies Inc Nucleolus Bright Red (60 NMOL) allows for high localization in nucleolus, simply add the reagent to fixed cells to measure the senescence marker.
A Nucleolus is one of the structures forming a nucleus and becoming the starting point of the ribosomal biogenesis. There are many ribosomal RNA (rRNA) in the nucleolus and transcription of rRNA and processing are carried out. Whereas the morphological change of nucleolus is known as one of the indicators of cancer diagnosis, there have been some scientific reports describing the relation between nucleolus and DNA damage, autophagy, virus infection, and cellular senescence. Nucleolus Bright dyes are small molecules and they bind to RNA in the nucleolus to emit fluorescence. The nucleolus can be observed without any washing steps after staining with Nucleolus Bright dyes. We recommend to co-stain with DAPI in order to image the nucleolus clearly. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
New England Biolabs, Inc. Blue Prestained Protein Standard, Broad Range (11-250 kDa) – 750 gel lanes
The Blue Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed.