The Nano ITC Test kit contains DDI H2O, Ca2+ and EDTA in 10mM MES buffer, pH6.0. There is sufficient volume of each reagent to perform three separate ITC titration experiments. Reagents should only be used with other reagents having the same Lot Number (Lot No.). The ITC Test Kit also comes with a CD containing the Test Kit Instruction Manual, Getting Started Guide, and Safety Data Sheets (SDS) for all chemicals in the Kit. The Getting Started Guide also contains the necessary information for proper loading and cleaning. All Nano ITC and Affinity ITC instruments are compatible with these reagents. This test kit is not included with any Nano or Affinity ITC instruments and should be a separate line item on any quote or order.