PCR Equipment and Supplies
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Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | SYBR |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Thermo Scientific™ Fluorescein-12-dUTP Solution (1 mM)
Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.
Thermo Scientific™ RevertAid RT Reverse Transcription Kit
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.
Thermo Scientific™ T4 DNA Polymerase (5 U/μL)
Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA.
| Polymerase | T4 DNA Polymerase |
|---|---|
| Concentration | 5 U/μL |
Thermo Scientific™ Oligo(dT)18 Primer
Thermo Scientific Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.
| Shipping Condition | Dry Ice |
|---|---|
| Primer Length | 18-mer |
| Form | Liquid |
| Product Type | Primer |
| Primer | Oligo dT |
| Concentration | 0.5 μg/μL |
| For Use With (Application) | cDNA Synthesis |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Tissue |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion Site-Directed Mutagenesis Kit
Generate mutations with this fast and reliable system.
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 2X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ Detection Enhancer, for real-time PCR
Formulated to improve template amplification in real-time PCR (qPCR) workflows
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Product Type | Detection Enhancer |
| For Use With (Equipment) | 7500 System |
| Sample Type | Viral Samples |
| Reaction Speed | Standard |
| For Use With (Application) | Real Time PCR (qPCR) |
| PCR Method | 1-step RT-qPCR |
| Product Line | Applied Biosystems |
| Detection Method | Primer-probe |
Invitrogen™ Oligo (dT) Primer (50 μM)
These are the same Invitrogen primers that are currently included in the SuperScript IV Kit (Cat. No. 18091050).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Frozen, Dry Ice |
|---|---|
| Target | cDNA |
| Content And Storage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
| Format | Kit |
| Sample Type | Mammalian Cells |
| Isolation Technology | Direct cell lysis |
| Kit Type | Direct Cell Lysis RT-qPCR |
| Purification Target | RNA |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | Yes |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
| Concentration | 200 U/μL |
|---|
Applied Biosystems™ VetMAX™ PRRSV 3.0 Controls
VetMAX™ PRRSV 3.0 Controls are for use in multiplex, real-time reverse transcription PCR (RT-PCR) assays targeting North American and European porcine reproductive and respiratory syndrome virus (PRRSV) and Xeno RNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Mutation Generation System Kit
Perform functional analysis of proteins with these transposon-based tools.