PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
GC-Rich PCR Performance	High
Content And Storage	• Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl₂ solution and DMSO Store at –20°C.
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Separate Components
Product Type	High-Fidelity PCR Kit
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
For Use With (Application)	Standard PCR,High-fidelity PCR

Applied Biosystems™ VetMAX™ PRRSV 3.0 Controls

VetMAX™ PRRSV 3.0 Controls are for use in multiplex, real-time reverse transcription PCR (RT-PCR) assays targeting North American and European porcine reproductive and respiratory syndrome virus (PRRSV) and Xeno RNA.

Pricing & Availability

Promotions Available

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Pricing & Availability
Specifications

GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Product Type	DNA Polymerase With Buffer I
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Hot-start PCR
Exonuclease Activity	5' - 3'

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	Maxima
Final Product Type	First-Strand cDNA
Quantity	Each
For Use With (Application)	Real Time PCR (qPCR)
Format	Kit
Size (Final Product)	Up to 20 kb
Reaction Format	Separate components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	50°C to 55°C
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

Pricing & Availability
Specifications

Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	Separate Components
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Hot-start PCR

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

The TaqMan™ Cells-to-CT™ Express Kit enables rapid, reproducible gene expression analysis directly from cultured cells—allowing you to skip RNA extraction entirely. By removing extraction steps, the workflow reduces sample loss, shortens time to results, and improves consistency across experiments and operators.

Pricing & Availability
Specifications

Shipping Condition	Frozen, Dry Ice
Target	cDNA
Content And Storage	Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format	Kit
Sample Type	Mammalian Cells
Isolation Technology	Direct cell lysis
Kit Type	Direct Cell Lysis RT-qPCR
Purification Target	RNA
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
High-throughput Compatibility	Yes
PCR Method	2-step RT-qPCR
Detection Method	TaqMan

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl₂.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	2X
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Cloning,Hot-start PCR

Thermo Scientific™ RevertAid RT Reverse Transcription Kit

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

Pricing & Availability

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, Power SYBR™ Green
Detection Method	SYBR

Promotions Available

Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit

Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.

Pricing & Availability

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Content And Storage	Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form	Liquid
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 6-plex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	8 hr., Assembled Reaction
Polymerase	Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format	Single-Tube
Passive Reference Dye	None
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Antibody-based
Shelf Life	Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line	TaqPath

Thermo Scientific™ dATP Solution (100 mM)

Thermo Scientific™ dATP (2'-deoxyadenosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.

Pricing & Availability

Promotions Available

Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR Starter Kit

The QuantStudio Absolute Q Digital PCR Starter Kit is designed to confirm the performance of the QuantStudio Absolute Q Digital PCR System either at the time of system installation or as part of future, user-defined system verification procedures.

Pricing & Availability

Thermo Scientific™ dsDNase

dsDNase is a heat inactivatable recombinant shrimp DNase used to remove genomic DNA contamination of RNA samples before reverse transcription.

Pricing & Availability

PCR Equipment and Supplies

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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ RevertAid RT Reverse Transcription Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ dATP Solution (100 mM) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

Thermo Scientific™ RevertAid RT Reverse Transcription Kit

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Thermo Scientific™ dATP Solution (100 mM)

Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)