PCR Supplies
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Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | MultiScribe |
| Size (Final Product) | Up to 7 kb |
| Reaction Format | Premixed Components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 to 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 37°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Label or Dye | SYBR Green |
| Product Line | SYBR |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Polymerase | AmpliTaq Gold DNA Polymerase |
| GC-Rich PCR Performance | High |
| Product Type | Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Product Type | Multiplex Kit |
|---|---|
| For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
| Sample Type | DNA |
| Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
| Product Line | SNaPshot |
| Detection Method | Primer-probe |
Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer
These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| PCR Method | qPCR,Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Invitrogen™ T4 DNA Polymerase
DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Polymerase | T4 DNA Polymerase |
| Content And Storage | T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C. |
| Description | DNA Polymerase |
| Hot Start | No |
| Exonuclease Activity | 3' - 5' |
SureTect™ Salmonella Species PCR Assay
Rapidly detect Salmonella species in food and environmental samples with the Thermo Scientific™ SureTect™ Salmonella Species PCR Assay.
Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - JAK2 12600
Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - BRAF 476
Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ PCR SuperMix
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ GeneAmp™ 10X PCR Gold Buffer & MgCl2
Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Format | Kit |
| Product Type | 10X PCR Gold Buffer |
| Concentration | 10X |
| For Use With (Application) | Standard PCR |
Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA)
Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Purity or Quality Grade | LD (Low DNA) |
| Quantity | 250 units |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq DNA Polymerase |
| No. of Reactions | 200 Reactions |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Format | Kit |
| Sample Type | PCR Products |
| Isolation Technology | Silica Spin Column |
| Run Time | 10 min. |
Thermo Scientific™ dNTP/dUTP Mix
Thermo Scientific dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM.