PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
InhibitorToleranceSimple	Medium
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	PowerUP™, SYBR™

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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
InhibitorToleranceSimple	High
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	8 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudio™ Absolute Q Digital PCR System, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	Applied Biosystems™

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Product Type	High-Fidelity DNA Polymerase
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Standard PCR,High-fidelity PCR

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Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Content And Storage	Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Genotyping
PCR Method	qPCR
Multiplex Capability	Up to 3-plex, Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, StepOne™ System
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath™

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type	RNA
Reaction Speed	Standard
Product Line	AgPath-ID™, Ambion™

Invitrogen™ PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Kit
Sample Type	PCR Products
Isolation Technology	Silica Spin Column
Run Time	10 min.

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion Flash II DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Color	Colorless
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Thermo Scientific™ dsDNase

dsDNase is a heat inactivatable recombinant shrimp DNase used to remove genomic DNA contamination of RNA samples before reverse transcription.

Pricing & Availability

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl₂

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Pricing & Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
Fidelity (vs. Taq)	1X
Size (Final Product)	5 kb or less
Product Type	DNA Polymerase
Overhang	3'-A
Concentration	5 U/μL
Exonuclease Activity	5' - 3'

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,DNA Quantitation,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	Not Applicable
Sensitivity	Down to 10 copies
Format	Tube
Passive Reference Dye	None
For Use With (Equipment)	QuantStudio™ 6 Pro or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, QuantStudio™ Absolute Q Digital PCR System, ViiA™ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier
Sample Type	DNA, RNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Product Line	TaqPath™

Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
For Use With (Application)	Hot-start PCR

Applied Biosystems™ GeneAmp™ dNTP Blend (2.5 mM ea)

GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Form	Liquid
Concentration	10 mM
For Use With (Application)	Standard PCR
Product Line	GeneAmp™

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2X
For Use With (Application)	Standard PCR,High-fidelity PCR

Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion Hot Start II High-Fidelity DNA Polymerase
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

PCR Supplies

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