Direct PCR Reagents and Kits
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Filtered Search Results
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
| Reverse Transcriptase | M-MLV |
| Size (Final Product) | 7 kb or less |
| Final Product Type | cDNA |
| Sample Type | Mammalian Cells |
| Purification Time | 2 hr. |
| Optimal Reaction Temperature | 42°C |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
| Product Line | Ambion, Cells-to-cDNA |
Thermo Scientific™ Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phusion Blood II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Reaction Format | Separate Components |
| Product Type | Direct PCR Kit |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| Starting Material | DNA |
| PCR Method | Direct PCR |
Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Purification Time | 7 min. |
| Starting Material | 1-10 Cells |
| For Use With (Application) | RT-PCR |
| PCR Method | 2-step RT-qPCR |
| Product Line | Ambion, Cells-to-CT |
| Detection Method | TaqMan |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | Direct PCR |
| Polymerase | Phusion Blood II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ PureLink™ PCR Purification Binding Buffer B2
PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Product Type | Multiplex Kit |
|---|---|
| For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
| Sample Type | DNA |
| Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
| Product Line | SNaPshot |
| Detection Method | Primer-probe |
Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Purity or Quality Grade | UP (Ultra Pure) |
| Reverse Transcriptase | M-MLV |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Polymerase | DNA Polymerase |
| Format | Tube |
| Product Type | Gene Expression Cells-to-C Kit |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Cell |
| Reaction Speed | Standard |
| Product Line | Ambion, Cells-to-CT, TaqMan |
Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Frozen, Dry Ice |
|---|---|
| Target | cDNA |
| Product Type | Direct Cell Lysis to RT-qPCR Kit |
| Sample Type | Hepatocytes, HepaRG, HepG2 |
| Isolation Technology | Direct Cell Lysis |
| Purification Target | RNA |
| Starting Material | Up to 100,000 Cultured Cells |
| High-throughput Compatibility | Yes |
Invitrogen™ RNA Control 250 with Manual
The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | RNA Control and THE RNA Storage Solution should be stored at –20°C |
|---|---|
| Format | Tube |
| Label or Dye | Unconjugated |
| Product Line | Ambion |