Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

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Invitrogen™ PureLink™ Pro 96 PCR Purification Kit

The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.

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Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

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Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) • ChargeSwitch™ Purification Buffer (N5) • ChargeSwitch™ Wash Buffer (W12) • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Run Time	5 min
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	High-throughput Compatible
Product Line	ChargeSwitch

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2X
Concentration	2X
For Use With (Application)	Gene Expression
High-throughput Compatibility	High-throughput Compatible
PCR Method	2-step RT-qPCR
Detection Method	SYBR
Format	Tube
Product Type	Fast Advanced Cells-to-Ct Kit
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Purification Target	Lysate
Starting Material	10-100,000 Cells
Product Line	Cells-to-CT

Invitrogen™ RNA Control 250 with Manual

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

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Specifications

Content And Storage	RNA Control and THE RNA Storage Solution should be stored at –20°C
Format	Tube
Label or Dye	Unconjugated
Product Line	Ambion

Invitrogen™ TaqMan™ Cells-to-C_T™ Control Kit

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

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Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

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Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.

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Thermo Scientific™ MuA Transposase

Transposon-based tools for functional analysis of proteins.

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Invitrogen™ PureLink™ PCR Micro Kit

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Separation Range	100 bp to 12 kb
Content And Storage	Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume	125 μL
Isolation Technology	Silica Spin Column
Elution Volume Max	20 μL
For Use With (Application)	Cloning,Standard PCR
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Target	DNA from Enzymatic Reactions (e.g. PCR)
Format	Spin Column
Product Type	PCR Micro Kit
Run Time	10 min.
Elution Volume Min	5 μL
Product Line	PureLink

Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit

The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Frozen, Dry Ice
GC-Rich PCR Performance	High
Form	Liquid
Isolation Technology	Direct Cell Lysis
For Use With (Application)	Gene Expression
High-throughput Compatibility	Yes
PCR Method	2-step RT-qPCR
Detection Method	TaqMan
Target	cDNA
Format	Kit
Product Type	Direct Cell Lysis to RT-qPCR Kit
Sample Type	Hepatocytes, HepaRG, HepG2
Reaction Speed	10 min.
Label or Dye	ROX
Purification Target	RNA
Starting Material	Up to 100,000 Cultured Cells

Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Pricing & Availability
Specifications

Product Type	Multiplex Kit
For Use With (Equipment)	3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type	DNA
Technique	SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line	SNaPshot
Detection Method	Primer-probe

Invitrogen™ TaqMan™ Fast Cells-to-C_T™ Kit

The TaqMan™ Fast Cells-to-C_T™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

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Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phusion Blood II DNA Polymerase
GC-Rich PCR Performance	High
Reaction Format	Separate Components
Product Type	Direct PCR Kit
Sample Type	Blood
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2 U/μL
Starting Material	DNA
PCR Method	Direct PCR

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

The Ambion Single Cell-to-C_T™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Purification Time	7 min.
Starting Material	1-10 Cells
For Use With (Application)	RT-PCR
PCR Method	2-step RT-qPCR
Product Line	Ambion, Cells-to-CT
Detection Method	TaqMan

Invitrogen™ Cells-to-C_T Bulk RT Reagents

Cells-to-C_T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-C_T™ Kit (20X RT enzyme mix and 20X RT buffer).

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Direct PCR Reagents and Kits

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Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Cells-to-CT Bulk RT Reagents Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit

Invitrogen™ TaqMan™ Cells-to-C_T™ Control Kit

Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit

Invitrogen™ TaqMan™ Fast Cells-to-C_T™ Kit

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

Invitrogen™ Cells-to-C_T Bulk RT Reagents