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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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16 – 30 of 247 results
16

Thermo Scientific™ Mutation Generation System Kit

Perform functional analysis of proteins with these transposon-based tools.

EDGE
17

Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.

EDGE ENCOMPASS_PREFERRED
18

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit
GREENER_CHOICE

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

EDGE ENCOMPASS_PREFERRED
19

Invitrogen™ PureLink™ PCR Micro Kit

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Room Temperature
Separation Range 100 bp to 12 kb
Content And Storage Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume 125 μL
Isolation Technology Silica Spin Column
Elution Volume Max 20 μL
For Use With (Application) Cloning,Standard PCR
High-throughput Compatibility Not High-throughput Compatible (Manual)
Target DNA from Enzymatic Reactions (e.g. PCR)
Format Spin Column
Product Type PCR Micro Kit
Run Time 10 min.
Elution Volume Min 5 μL
Product Line PureLink
20

Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
GREENER_CHOICE

Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.

EDGE ENCOMPASS_PREFERRED
21

Invitrogen™ RNA Control 250 with Manual

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

EDGE ENCOMPASS_PREFERRED
Content And Storage RNA Control and THE RNA Storage Solution should be stored at –20°C
Format Tube
Label or Dye Unconjugated
Product Line Ambion
22

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit
GREENER_CHOICE

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

EDGE ENCOMPASS_PREFERRED
23

Thermo Scientific™ MuA Transposase

Transposon-based tools for functional analysis of proteins.

EDGE
24

Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit
GREENER_CHOICE

The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Frozen, Dry Ice
GC-Rich PCR Performance High
Form Liquid
Isolation Technology Direct Cell Lysis
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Target cDNA
Format Kit
Product Type Direct Cell Lysis to RT-qPCR Kit
Sample Type Hepatocytes, HepaRG, HepG2
Reaction Speed 10 min.
Label or Dye ROX
Purification Target RNA
Starting Material Up to 100,000 Cultured Cells
25

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Content And Storage 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at
-20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase M-MLV
Size (Final Product) 7 kb or less
Final Product Type cDNA
Sample Type Mammalian Cells
Purification Time 2 hr.
Optimal Reaction Temperature 42°C
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Product Line Ambion, Cells-to-cDNA
26
Promotions Available

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Polymerase DNA Polymerase
Format Tube
Product Type Gene Expression Cells-to-C Kit
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cell
Reaction Speed Standard
Product Line Ambion, Cells-to-CT, TaqMan
27

Invitrogen™ PureLink™ PCR Purification Binding Buffer B2

PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.

EDGE ENCOMPASS_PREFERRED
28

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

EDGE ENCOMPASS_PREFERRED
29

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit
GREENER_CHOICE

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.

EDGE ENCOMPASS_PREFERRED
30

Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

EDGE ENCOMPASS_PREFERRED
Product Type Multiplex Kit
For Use With (Equipment) 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type DNA
Technique SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line SNaPshot
Detection Method Primer-probe