Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

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Invitrogen™ RNA Control 250 with Manual

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

Pricing & Availability
Specifications

Content And Storage	RNA Control and THE RNA Storage Solution should be stored at –20°C
Format	Tube
Label or Dye	Unconjugated
Product Line	Ambion

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, Power SYBR™ Green
Detection Method	SYBR

Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.

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Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents

Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.

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Promotions Available

Invitrogen™ PureLink™ PCR Micro Kit

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Separation Range	100 bp to 12 kb
Content And Storage	Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume	125 μL
Isolation Technology	Silica Spin Column
Elution Volume Max	20 μL
For Use With (Application)	Cloning,Standard PCR
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Target	DNA from Enzymatic Reactions (e.g. PCR)
Format	Spin Column
Product Type	PCR Micro Kit
Run Time	10 min.
Elution Volume Min	5 μL
Product Line	PureLink

Thermo Scientific™ MuA Transposase

Transposon-based tools for functional analysis of proteins.

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Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

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Invitrogen™ TaqMan™ Cells-to-C_T™ Control Kit

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

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Promotions Available

Invitrogen™ PureLink™ PCR Purification Binding Buffer B2

PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.

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Promotions Available

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

The Ambion Single Cell-to-C_T™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Purification Time	7 min.
Starting Material	1-10 Cells
For Use With (Application)	RT-PCR
PCR Method	2-step RT-qPCR
Product Line	Ambion, Cells-to-CT
Detection Method	TaqMan

Invitrogen™ Cells-to-C_T Bulk RT Reagents

Cells-to-C_T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-C_T™ Kit (20X RT enzyme mix and 20X RT buffer).

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Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.

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Promotions Available

Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) • ChargeSwitch™ Purification Buffer (N5) • ChargeSwitch™ Wash Buffer (W12) • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Run Time	5 min
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	High-throughput Compatible
Product Line	ChargeSwitch

Promotions Available

Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit

The TaqMan™ Gene Expression Cells-to-C_T™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	M-MLV
PCR Method	2-step RT-qPCR
Detection Method	Primer-probe
Polymerase	DNA Polymerase
Format	Tube
Product Type	Gene Expression Cells-to-C Kit
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cell
Reaction Speed	Standard
Product Line	Ambion, Cells-to-CT, TaqMan

Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Pricing & Availability
Specifications

Product Type	Multiplex Kit
For Use With (Equipment)	3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type	DNA
Technique	SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line	SNaPshot
Detection Method	Primer-probe

Direct PCR Reagents and Kits

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Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Cells-to-CT Bulk RT Reagents Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents

Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

Invitrogen™ TaqMan™ Cells-to-C_T™ Control Kit

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

Invitrogen™ Cells-to-C_T Bulk RT Reagents

Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit