Real Time PCR Master Mixes
Sigma Aldrich Fine Chemicals Biosciences fastStart Universal SYBR G
fastStart(TM) Universal SYBR(R) Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX. SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye which is included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence. Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart(TM) Taq DNA Polymerase make the modified enzyme inactive at room temperature (15 to 25C). Therefore there is no elongation during the period when primers can non-specifically bind. fastStart(TM) Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
Sigma Aldrich Fine Chemicals Biosciences SYBR(R) Green I nucleic acid gel stain 10,000 x in DMSO | 163795-75-3 | MFCD00284715 | 1ML
SYBR(R) Green I nucleic acid gel stain 10,000 x in DMSO | 163795-75-3 | MFCD00284715 | 1ML
Biotium Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn)
Fast-Plus EvaGreen Master Mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt analysis of PCR amplicons. It delivers clean PCR product even with the most challenging samples tested. The mix contains environmentally safe EvaGreen dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq. Although the master mix is formulated for qPCR using a fast cycling protocol, it is also compatible with qPCR using a regular cycling protocol. High ROX reference dye is included in the mix for well-to-well fluorescence normalization in ABI and other instrument platforms. This product has sufficient reagent for 200 reactions.
ABclonal Technology Genious 2X SYBR Green Fast qPCR Mix
Real-time Quantitative PCR (qPCR) is a powerful technique in detecting initial DNA input in a PCR reaction by fluorescence signal accumulation. ABclonal Genious 2X SYBR Green Fast qPCR Mix is a fluorescence reagent for qPCR reactions using the SYBR(R) Green I. This product provides real-time data on DNA amplification during PCR by performing quantitative fluorescence signal detection on SYBR(R) / FAM channels. This product uses the antibody-based hot-start Taq enzyme for amplification, which greatly improves the specificity of the product while ensuring the amplification effect. At the same time, by optimizing the qPCR Mix Buffer system, the product is suitable for multiple species, providing a powerful tool for multidisciplinary experimental needs. This product is a 2X pre-mix enzyme, contains all the components required for qPCR except primers and templates, providing great convenience for experimental manipulation.
INTACT GENOMICS INC FastAmp qRPA SYBR Kit, 25 Reactions
IG FastAmp qRPA SYBR kit that contains ready-to-use master mixes with all components except primers and template for isothermal amplification, detection, and quantification of DNA using Recombinase Polymerase Amplification. With this kit, RPA progress can be monitored in real-time and quantified using standardized template concentrations. The FastAmp qRPA SYBR kit is compatible with high throughput (96-well or 384-well) format fluorescent readers for faster results in just 30 minutes at constant 37⁰C. Now, primer sets can be validated by screening in parallel for isothermal RPA assay optimization with Flexible endpoint detection compatibility. Kit Includes: ,FastAmp qRPA SYBR Reaction Master Mix, IG RPA Enzyme Mixture v2 (4x), 280 mM Magnesium Acetate (MgOAc), Nuclease-free Water. Storage Temperature: -20°C
Sigma Aldrich Fine Chemicals Biosciences FastStart SYBR Green Maste
FastStart SYBR Green Master Instructions For UseFastStart(TM) SYBR(R) Green Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycler(R) instruments. This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument suitable PCR primers and a hydrolysis probe FastStart(TM) TaqMan(R) Probe Master allows very sensitive detection and quantification of defined DNA sequences.SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence.Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart(
Apexbio Technology LLC 2SYBR Green qPCR Master Mix, 2500rxn (20 ul reaction)
2SYBR Green qPCR Master Mix is a ready-to-use 2X master mix optimized for highly specific and sensitive real-time quantitative PCR using SYBR Green I and Hot Start Taq DNA Polymerase. SYBR Green I is a fluorescent double-stranded DNA binding dye that tracks double-stranded DNA amplified in PCR. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC RT SuperMix for qPCR, 50 rxn (20 uL/rxn). Cas: MFCD: NA. High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method
RT SuperMix for qPCR is the premixed solution for reverse transcription reaction, based on Reverse Transcriptase (Cat. No. K1071). Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, Reverse Transcriptase reduces RNase H activity and increases thermal stability. Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures.
Alkali Scientific Radiant™ SYBR Green Fluorescein qPCR Kit, 500 Reactions, (5 x 1mL)
Radiant™ SYBR Green Flourescein qPCR Kit: Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed. Novel hot-start chemistry for improved specificity and sensitivity. Broad range detection for increased reliability in low-copy assays Versatile 2X mixes, suitable for both ultra-fast and standard cycling methods Compatible with BioRad iCycler™, MyIQ™ & IQ5™
Biotium Forget-Me-Not™ Universal Probe Master Mix with ROX (500 reactions)
High-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. Suitable for hydrolysis probes (TaqMan and dual-Labeled BHQ probes) and displacement probes. Excellent concordance of singleplex and multiplex. Standard and fast protocol compatible.
Apexbio Technology LLC UNI. 2X PROBE QPCR MA. MIX 5ML
UNI. 2X PROBE QPCR MA. MIX 5ML APEXBIO TECHNOLOGIES
Roche Diagnostics KAPA SYBR FAST qPCR Kits - 500 x 20 uL reactions (Universal), 5 mL
Kits include KAPA SYBR FAST qPCR Master Mix (2X), which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. *This item is non returnable.
Biotium Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)
Fast Evagreen qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes. Fast Evagreen qPCR Master Mix is a 2X master mix, and is supplied with a separate vial of ROX reference dye, for use with high ROX, low ROX, or no ROX instruments. EvaGreen dye is a unique DNA-binding dye with features ideal for both qPCR and melt curve analysis. EvaGreen dye binds to dsDNA via a novel “release-on-demand” mechanism, which permits the use of a relatively high dye concentration in qPCR without PCR inhibition. This product has sufficient reagent for 100 reactions.
Biotium Fast Plus EvaGreen qPCR Master Mix with High Rox (trial size, 100 rxn)
Fast-Plus EvaGreen Master Mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt analysis of PCR amplicons. It delivers clean PCR product even with the most challenging samples tested. The mix contains environmentally safe EvaGreen dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq. Although the master mix is formulated for qPCR using a fast cycling protocol, it is also compatible with qPCR using a regular cycling protocol. High ROX reference dye is included in the mix for well-to-well fluorescence normalization in ABI and other instrument platforms. This trial size has sufficient reagent for 100 reactions.
Biotium Forget-Me-Not™ EvaGreen qPCR Master Mix (High ROX) (100 reactions)
Forget-Me-Not™ EvaGreen qPCR Master Mix is a high performance, hot-start EvaGreen dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ master mix has a light blue master mix color which helps prevent pipetting mistakes, saving time and reagents. The master mix features EvaGreen Dye which is suitable for HRM analysis and is safer, more environmentally friendly, and more sensitive than SYBR Green. The kit also features Cheetah™ Hotstart Taq for fast cycling protocols and High ROX reference dye. This trial size has sufficient reagent for 100 PCR reactions.