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Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
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31
Promotions Available

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq DNA Polymerase
Format Tube
Product Type Fast Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Product Line AmpErase, TaqMan
32

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG

Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
33
Promotions Available

Applied Biosystems™ TaqMan™ Multiplex Master Mix
GSA_VA

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

EDGE ENCOMPASS_PREFERRED
Polymerase AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Multiplex Master Mix
Sample Type DNA
Reaction Speed Fast
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method Multiplex qPCR
Product Line TaqMan
Detection Method Primer-probe
34
Promotions Available

Applied Biosystems™ TaqMan™ Genotyping Master Mix
GSA_VA

Optimized for end-point fluorescence detection in SNP genotyping applications.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Genotyping
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Genotyping Target Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Product Type Genotyping Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Sample Type DNA (Genomic)
Reaction Speed Standard
Technique SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Product Line TaqMan
35
Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

EDGE ENCOMPASS_PREFERRED
Polymerase AmpliTaq Gold DNA Polymerase
Purity or Quality Grade UP (Ultra Pure)
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
36
Promotions Available

Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)
GSA_VA

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Product Type PreAmp Master Mix
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type cDNA
For Use With (Application) Pre-amplification
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
37
Promotions Available

Applied Biosystems™ TaqMan™ GTXpress™ Master Mix

Combines AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Concentration 2X
For Use With (Application) Genotyping
PCR Method qPCR
38
Promotions Available

Applied Biosystems™ TaqMan™ Universal PCR Master Mix
GSA_VA

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

EDGE ENCOMPASS_PREFERRED
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
39
Promotions Available

Applied Biosystems™ Fast SYBR™ Green Master Mix

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Label or Dye SYBR Green
Product Line SYBR
40
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
41

Applied Biosystems™ Absolute Q™ MAP16 Plate Kit and Absolute Q™ Universal DNA dPCR Master Mix

Eliminate the need to design custom assays and choose from over 20 million predesigned assays. Includes the Absolute Q MAP16 plate kit plus the Absolute Q Universal DNA dPCR master mix which is compatible with a wide range of applications and difficult targets.

ENCOMPASS_PREFERRED
42

Applied Biosystems™ Custom OpenArray™ Preamp Pool

Custom OpenArray™ Preamp Pool

ENCOMPASS_PREFERRED
43

Applied Biosystems™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Form Liquid
For Use With (Application) Genotyping
PCR Method qPCR
Detection Method HRM
Polymerase AmpliTaq Gold 360 DNA Polymerase
Genotyping Target SNPs (Unknown or Numerous), Epigenetic Targets
Product Type HRM Master Mix
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Label or Dye High Resolution Melting (HRM),SYTO 9
Technique HRM (High Resolution Melting) Analysis, Mutation Scanning
Product Line MeltDoctor
44

MP Biomedicals™ RapidScript™ Probe One-Step RT-qPCR Kit

Use the RapidScript™ Probe One-Step RT-qPCR Kit for simple, one-step RT-qPCR.

45
Promotions Available

Invitrogen™ EXPRESS qPCR Supermix, with premixed ROX

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays.

ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Content And Storage EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format Tube
Product Type Express qPCR Supermix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-probe