Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Roche Diagnostics HotStart ReadyMix with dye | 100x25uL Reactions
KAPA2G Fast PCR Kits contain a second-generation (2G) DNA polymerase enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-typeTaqpolymerase. In addition to speed, KAPA2G Fast PCR Kit provides higher yields and sensitivity than competitor enzymes across a broad range of targets. *This item is non returnable.
Bioss SARSCovid Coronavirus NP
SARS Covid Coronavirus NP Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications WB ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 500 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
Biotium PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx™
This PMAxx™-PCR kit is designed for selective detection of viable bacteria from a specific strain using PMAxx™ dye and real-time PCR. This kit provides primers for specific amplification of the Salmonella enterica invA gene. The kit has sufficient reagents for treating of 80 bacterial cultures and performing 200 PCR reactions with PMAxx™. PMAxx™ is a new and improved version of PMA designed by Biotium scientists to be a superior alternative to PMA. While PMA is generally effective at differentiating between live and dead bacteria by qPCR, it does not completely eliminate PCR products from dead cell DNA. This could potentially give false positive results. Biotium’s new dye PMAxx™ is much more effective at eliminating PCR amplification of dead cell DNA, and therefore provides the best discrimination between live and dead bacteria. Salmonella enterica is a gram-negative bacteria that causes the food-borne illness salmonellosis.
Boca Scientific Inc e-Myco PCR Mycoplasma Detection Kit, 48 Tubes
The e-Myco Mycoplasma PCR Detection Kit consists of a set of primers designed to detect the presence of mycoplasma and determine the species. This kit can detect five common cell culture-infecting types of mycoplasma. The species of mycoplasma can be determined by sequencing the amplified products. 49 species can be detected. Each detection tube contains all components required for PCR in a dried and stable pellet format, including i-StarTaq DNA Polymerase, dNTPs, 10x Buffer, Mycoplasma primers, 8-methoxypsoralen, and an internal control for Mycoplasma partial gene amplifications. The reaction can begin by simply adding the template DNA or samples to be tested. PCR with this primer set is based on conserved 16S rRNA and delivers detection results in only a few hours. This is far more efficient than the conventional detection methods that involve culturing samples on selective media and require at least 1 week to obtain results.
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Eagle Biosciences Inc RCL(VSVG) Gene Copy Number Detection Kit (qPCR)
RCL(VSVG) Gene Copy Number Detection Kit (qPCR)
Meridian Life Science Inhibitor-Tolerant RT-qPCR Mix, 1ML
Meridian’s universal Inhibitor-Tolerant RT-qPCR is a 4x formulation ready-to-use quantitative RT-PCR supermix, containing all components except primers, probes and templates for direct RT-qPCR. It is designed for developing RT-qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various RT and qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.