Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
ACROBiosystems Plasmid resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
ATCC Balamuthia mandrillaris; CDC:V039
This parasitic protozoan was isolated from the brain tissue of a mandrill that died of amebic meningoencephalitis in the San Diego Zoo.
Meridian Life Science Lyo-Ready qPCR Mix 2.6x, 100ML
Lyo-Ready qPCR Mix, 2.6x is a higher concentration, glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2 and lyo-excipients. It is ideal for developing room temperature, lyophilized, multiplex qPCR diagnostic tests and is suited for use with microfluidic qPCR, where the initial volume needs to be smaller. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.
Meridian Life Science Air-Dryable qPCR Mix 4x, 50ML
Air-Dryable qPCR Mix (4x) is a glycerol-free qPCR mix containing an antibody-mediated hot-start Taq polymerase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients. It is used for the development of multiplex molecular diagnostic tests, enabling highly sensitive detection of DNA targets. The air drying does not affect the specificity or reproducibility of the assay but increases the flexibility of the volume of sample that can be added, increasing sensitivity and also makes it ideal for microfluidic qPCR and point of care platforms.
Meridian Life Science Fast qPCR Mix, 5ML
Fast qPCR Mix, 2x has been designed with the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a fast hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate, sensitive qPCR efficiency, even from low abundance targets and scarce samples. With high processivity and robustness, it delivers excellent results in both singleplex and multiplex qPCR assays and confers superior assay performance under fast thermal cycling conditions, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.
Alkali Scientific Radiant™ Probe 1-Step Hi-ROX qPCR Kit, 100 Reactions, (1 X 1mL 2X Probe 1-Step Hi-ROX qPCR Mix, 1 X 0.2mL 20X RTase with RNase inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Hi-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Hi-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ qPCR Genotyping Mix, Lo-Rox, 100 Reactions, (1 X 1mL)
Using the latest advancements in polymerase technology and advanced buffer chemistry Radiant™ Genotyping Mix Lo-Rox offers market leading performance with minimal optimization. Radiant™ Genotyping Mix Lo-Rox is a kit designed for use in dual-labeled probe based genotyping assays including Taqman, Molecular Beacon and Scorpion probe genotyping. Radiant™ Genotyping Mix is fully compatible with ABI TaqMan Pre-Designed SNP Genotyping assays. Radiant™ Genotyping Mix uses proprietary smallmolecular inhibitor technology that prevents formation of primer-dimers to improve reaction sensitivity and specificity. Highthroughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.
Meridian Life Science Lyo-Ready qPCR Buffer 2.5x, 100ML
Lyo-Ready qPCR Buffer 2.5x is a glycerol-free qPCR reaction buffer containing lyo-excipients, designed to deliver highly reproducible, accurate qPCR assay results under fast thermal cycling conditions. In order to produce lyophilized, ambient-temperature stable qPCR reagents, dNTP, MgCl2 and glycerol-free Taq DNA polymerase (such as MDX011 or MDX015) must be added prior to lyophilization.
Meridian Life Science Low DNA qPCR Mix, 5ML
Low DNA qPCR master mix contains a chemical hot-start Taq polymerase and highly optimized reaction buffer with dNTPs, MgCl2, stabilizers and PCR enhancers. The Taq polymerase is purified twice, before and after the addition of the chemical hot-start, this helps to remove trace amounts of DNA (known as bioburden) that come from the manufacturing of the DNA polymerase. In addition there is no bioburden that comes with antibody hot-start, making it ideal for low-copy number targets and also microbial DNA and fungal DNA detection assays. Low DNA qPCR master mix can also be used for fast, precise and highly reproducible genotyping of sequence variants, including loci, provides higher stringency and more specific binding of the allele-specific probes, with wider and clearer separation of allele cluster.
Immunology Consultants anti-Canine Coronavirus Antibody (Mouse Clone #6B8) - Monoclonal
Clone 6B8 targets a distinct epitope on Canine Coronavirus, providing sensitive, specific detection for virology research and assay development. The antibody performs reliably in ELISA, Western blotting, and antigen‑capture formats, offering strong affinity and low nonspecific binding. MCCV‑40A‑6B8 is well suited for monitoring viral exposure, evaluating vaccine responses, and characterizing CCoV antigen expression in clinical or experimental samples. Its reproducible performance ensures dependable results across varied assay conditions.
Meridian Life Science Fast qPCR Mix 5x, 40ML
Fast qPCR Mix, 5x has been designed with the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a fast hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate, sensitive qPCR efficiency, even from low abundance targets and scarce samples. With high processivity and robustness, it delivers excellent results in both singleplex and multiplex qPCR assays and confers superior assay performance under fast thermal cycling conditions, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.
Meridian Life Science Air-Dryable qPCR Mix 4x, 5ML
Air-Dryable qPCR Mix (4x) is a glycerol-free qPCR mix containing an antibody-mediated hot-start Taq polymerase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients. It is used for the development of multiplex molecular diagnostic tests, enabling highly sensitive detection of DNA targets. The air drying does not affect the specificity or reproducibility of the assay but increases the flexibility of the volume of sample that can be added, increasing sensitivity and also makes it ideal for microfluidic qPCR and point of care platforms.
Cytek Biosciences Inc CYBRFast™ qPCR Hi-ROX Master Mix
CYBRFast™ qPCR Hi-ROX Master Mix ..... Cat. No. 31-5102-0500R ..... Size 500 rxns
Cytek Biosciences Inc CYBRFast™ qPCR Lo-ROX Master Mix
CYBRFast™ qPCR Lo-ROX Master Mix ..... Cat. No. 31-5101-0100R ..... Size 100 rxns
Alkali Scientific Radiant™ Probe 1-Step No-ROX qPCR Kit, 300 Reactions, (3 X 1mL 2X Mix, 3 X 0.2mL 20X RTase)
Alkali Scientific offers Radiant™ Probe 1-Step No-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step No-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.