Dissociation Reagents
Dissociation Reagents
Amsbio LLC (formerly Genlantis) Detachin™ Cell Detachment Reagent
Gentle, rapid detachment of a wide range of cells
Amsbio LLC (formerly Genlantis) Papain Cell Culture Grade
High quality dissociation reagent prepares cells for plating
Amsbio LLC (formerly Genlantis) Subculturing Reagents
Functionally tested subculturing reagents for primary cells
STEMCELL Technologies DNase I Solution (1 mg/mL), Size: 1 mL
Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following thawing. The activity range is ≥ 2000 units per mg of protein.
STEMCELL Technologies ACCUTASE™, Size: 100 mL
A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. ACCUTASE™ does not contain mammalian or bacterial-derived products. Each lot of ACCUTASE™ is tested for sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
Lonza Walkersville Trypsin Neutralizing Solution, 100 mL
Trypsin Neutralizing Solution (TNS) is used to effectively neutralize Trypsin/EDTA. Lonza's TNS is availableindividually or as a component in our ReagentPack Subculture Reagents (CC-5034) which includes Trypsin and HEPES buffer.Content:1 x Bottle Trypsin Neutralizing Solution, 100 mL
Lonza Walkersville Trypsin/EDTA Solution, 100 mL
Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension.It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6-7.8.After treatment, trypsin activity can be halted by adding a serum media mixture or a trypsin inhibitor (from soybean, for example) in serum-free systems.Trypsin/EDTA is also a component of Lonza's ReagentPack Subculture Reagents kit (CC-5034) which includes Trypsin Neutralizing reagent and HEPES buffer.Content:1 x Bottle Trypsin/EDTA 100 mL
Sigma Aldrich Fine Chemicals Biosciences Liberase TM Research Grade, 10 mg
Liberase TM Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a medium concentration of Thermolysin, a non-clostridial neutral protease.
STEMCELL Technologies Dispase (5 U/mL), Size: 100 mL
Dispase is a protease that is suitable for the gentle dissociation of a wide variety of tissues. This product contains 5 U/mL Dispase II (neutral protease from Bacillus polymyxa) dissolved in Hanks’ Balanced Salt Solution.
Sigma Aldrich Fine Chemicals Biosciences Trypsin-EDTA solution 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2Â g EDTA . 4Na per liter of Hanks' Balanced Salt Solution with phenol red | MFCD00130286 | 500ML
Trypsin-EDTA solution 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2Â g EDTA . 4Na per liter of Hanks' Balanced Salt Solution with phenol red | MFCD00130286 | 500ML
STEMCELL Technologies Dispase (1 U/mL), Size: 100 mL
Dispase is a protease that is suitable for the gentle dissociation of a wide variety of tissues. Incubation of minced tissue with pre-warmed dispase and gentle agitation will liberate cells with minimal cell damage. Pre-warmed dispase can also be used to harvest cells from tissue culture plastic. Unlike trypsin, dispase is not inhibited by serum. Dispase activity is inhibited by EDTA and EGTA. Dispase should be removed from cell suspensions by centrifuging the cells followed by washing the cells with buffer or culture medium.
STEMCELL Technologies Collagenase/Hyaluronidase, Size: 10 mL
10X Collagenase/Hyaluronidase in Dulbecco’s Modified Eagle’s Medium (DMEM) for the enzymatic dissociation of human mammary tissue.
STEMCELL Technologies STEMdiff™ Cardiomyocyte Dissociation Kit, Size: 1 Kit
STEMdiff™ Cardiomyocyte Dissociation Kit includes STEMdiff™ Cardiomyocyte Dissociation Medium and STEMdiff™ Cardiomyocyte Support Medium. STEMdiff™ Cardiomyocyte Dissociation Medium can be used to harvest cardiomyocytes that have been differentiated from human pluripotent stem cells (hPSCs) using STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (Catalog #05010) or STEMdiff™ Atrial Cardiomyocyte Differentiation Kit (Catalog #100-0215) and maintained in STEMdiff™ Cardiomyocyte Maintenance Medium (Catalog #05010/05020). STEMdiff™ Cardiomyocyte Support Medium reduces stress on these cardiomyocytes during harvesting and replating, maintaining their viability and functional capacity for downstream applications and analyses.