Additional Selective and Differential Cell Culture Media
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NEOGEN Bile Salts No. 3, 10 kg
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Bile Salts No. 3 is a mixture of bile salts for use in preparing microbiological culture media in a laboratory setting. Several media containing Bile Salts No. 3 are recommended in standard methods for multiple applications. Bile Salts No. 3 is not intended for use in the diagnosis of disease or other conditions in humans.Bile Salts No. 3 is a refined bile salts mixture, comprising mainly sodium cholate and sodium desoxycholate. Its primary use is as a selective agent for the isolation of Gram-negative micro-organisms, inhibiting Gram-positive cocci. Bile Salts No. 3 is prepared especially for use in MacConkey Agar and Violet Red Bile Agar. This fraction of bile is highly active allowing maximum selection of organisms of enteric origin at relatively low concentrations (0.15%) and is soluble in distilled or deionized water and neutral to slightly alkaline in pH.For more information visit https://www.neogen.com/solutions/Microbiology/bile-salts-number-3
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NEOGEN Agar, Technical, 500 g
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Agar, Technical is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Technical is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to US Pharmacopeia (USP).Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperatures for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-technical
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NEOGEN Agar, Bacteriological No. 2, 10 kg
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Agar, Bacteriological No. 2 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-bacteriological-number-2
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NEOGEN Beef Extract Powder, 500 g
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Beef Extract Powder is a dehydrated extract of bovine tissue for use in preparing microbiological culture media in a laboratory setting. Beef Extract Powder is not intended for use in the diagnosis of disease or other conditions in humans.Beef Extract Powder is prepared and standardized for use in microbiological culture media, where it is generally used to replace infusion of meat. Culture media containing Beef Extract Powder are recommended for use in bacteriological examination of water, milk, and other materials, where uniform composition of media is important. Beef Extract Powder is relied upon for biochemical studies, particularly fermentation reactions because of its independence from fermentable substances. Several media containing Beef Extract Powder are recommended in standard methods for multiple application.For more information visit https://www.neogen.com/solutions/Microbiology/beef-extract-powder
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NEOGEN Malt Extract, 500 g
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Malt Extract is a dehydrated extract of malt for use in preparing microbiological culture media in a laboratory setting. Malt Extract is not intended for use in the diagnosis of disease or other conditions in humans.Malt Extract is a clarified, water-soluble extract of malted barley. Malt Extract is a useful ingredient of culture media designed for the propagation of yeasts and molds. This ingredient is suitable for yeasts and molds because it contains a high concentration of carbohydrates, particularly maltose. The approximate percentage of reducing sugars in Malt Extract is 60 – 63%.The high carbohydrate content makes malt extract very sensitive to overheating which results in darkening of the medium it is used in. Malt Extract is generally employed in culture media at concentrations between 10 to 100 grams per liter.For more information visit https://www.neogen.com/solutions/Microbiology/malt-extract
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NEOGEN Nutrient Agar 1.5%, 10 kg
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Nutrient Agar 1.5% is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Agar 1.5% is not intended for use in the diagnosis of disease or other conditions in humans. In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Agar is specified in many standard method procedures. Nutrient Agar 1.5% is a modification of Nutrient Agar. Nutrient Agar 1.5% is a general purpose medium, with a slightly alkaline pH. This medium contains 0.8% sodium chloride and can be used as a base for enrichment with blood, ascitic fluid, or other supplements for cultivating fastidious microorganisms.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-agar-1-5-percent
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NEOGEN Reinforced Clostridial Agar, 500 g
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Reinforced Clostridial Agar can be used for the enumeration of anaerobes by pour plate, shake tube or membrane filtration methods, and is not intended for use in the diagnosis of disease or other conditions in humans. This is a solidified version of Reinforced Clostridial Medium (NCM0102) and can be used for the enumeration of anaerobes by pour plate, shake tube or membrane filtration methods. When solidified in tubes or bottles with minimal head space it can be used for anaerobic culture without the need for anaerobic atmosphere.For more information visit https://www.neogen.com/solutions/Microbiology/reinforced-clostridial-agar
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NEOGEN Meat Peptone No. 1, 500 g
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Meat Peptone No.1 is an enzymatic digest of animal tissue used in preparing microbiological culture media in a laboratory setting. Meat Peptone No.1 is not intended for use in the diagnosis of disease or other conditions in humans.Meat Peptone No. 1 was developed as an alternate nitrogen and vitamin source in dehydrated culture media to accommodate varying nutritional requirements.For more information visit https://www.neogen.com/solutions/Microbiology/meat-peptone-number-1
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NEOGEN Wort Agar, 500 g
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Wort Agar is for the enumeration of yeasts and molds in butter, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium can be modified to enable it to isolate osmophilic yeasts from soft drinks and sugar products by the addition of high concentrations of sucrose and glucose.For more information visit https://www.neogen.com/solutions/Microbiology/wort-agar
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NEOGEN Meat Peptone No. 1, 10 kg
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Meat Peptone No.1 is an enzymatic digest of animal tissue used in preparing microbiological culture media in a laboratory setting. Meat Peptone No.1 is not intended for use in the diagnosis of disease or other conditions in humans.Meat Peptone No. 1 was developed as an alternate nitrogen and vitamin source in dehydrated culture media to accommodate varying nutritional requirements.For more information visit https://www.neogen.com/solutions/Microbiology/meat-peptone-number-1
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NEOGEN CLED Medium (Bevis Modification), 500 g
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CLED Medium (Bevis Modification) is for the cultivation of coliforms, staphylococci and streptococci, and is not intended for use in the diagnosis of disease or other conditions in humans.Bevis modified Mackey and Sandys original medium by introducing a double indicator to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci. The swarming of Proteus spp. is inhibited. Neogen’s NCM0220 CLED Medium will grow many of the more demanding streptococci of Lancefield groups A, B, C, G and F. This medium may not grow Pasteurella spp. or halophilic organisms.For more information visit https://www.neogen.com/solutions/Microbiology/cled-medium-bevis-modification
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NEOGEN Iron Sulphite Agar, 500 g
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Iron Sulphite Agar is a medium for the detection of thermophilic anaerobic organisms causing sulphide spoilage in food, and is not intended for use in the diagnosis of disease or other conditions in humans.This formulation is a modification of Cameron Sulphite Agar, which was developed by the National Canners Association of America (now the Grocery Manufacturers Association). Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes. Beerens, and later Mossel, demonstrated that some strains of C. sporogenes would not tolerate sodium sulphite levels of 0.1%. Mossel further observed that reducing sulphite content to 0.05% improved detection of these strains.For more information visit https://www.neogen.com/solutions/Microbiology/iron-sulphite-agar
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NEOGEN Casein Digest, 500 g
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Casein Digest is an enzymatic digest of casein for use in preparing microbiological culture media in a laboratory setting. Casein Digest is not intended for use in the diagnosis of disease or other conditions in humans.Casein is the main protein of milk and a rich source of amino nitrogen. Casein Digest was developed for use in molecular genetic media formulations, including NZCYM Broth, NZYM Broth, and NZM Broth. The enriched formulations of NZ media are used to cultivate recombinant strains of E. coli and propagating theta bacteriophages.For more information visit https://www.neogen.com/solutions/Microbiology/casein-digest
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NEOGEN Enzymatic Digest of Soy No. 2, 10 kg
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Enzymatic Digest of Soy No. 2 is an enzymatic digest of soybean meal for use in preparing microbiological culture media in a laboratory setting. Enzymatic Digest of Soy No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.Enzymatic Digest of Soy No. 2 is an enzymatic hydrolysate of soybean meal prepared under controlled conditions for use in microbiological procedures. Enzymatic Digest of Soy No. 2 is recommended for use in media for the cultivation of a large variety of organisms, including fungi and microbiological assay media. The nitrogen source in Enzymatic Digest of Soy No. 2 contains naturally occurring, high concentrations of vitamins and carbohydrates of soybean. Due to its high carbohydrate content, Enzymatic Digest of Soy No. 2 cannot be used for fermentation studies.For more information visit https://www.neogen.com/solutions/Microbiology/enzymatic-digest-of-soy-number-2
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NEOGEN Oxbile (Oxgall), 500 g
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Oxbile (Oxgall) is dehydrated bile for use in preparing microbiological culture media in a laboratory setting. Oxbile (Oxgall) is not intended for use in the diagnosis of disease or other conditions in humans. Oxbile is manufactured from large quantities of fresh bile by rapid evaporation of the water content. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials. Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens.For more information visit https://www.neogen.com/solutions/Microbiology/oxbile-oxgall
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