Additional Selective and Differential Cell Culture Media
NEOGEN WL Nutrient Agar, 5 kg
W-L Nutrient Agar is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations. W-L Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans. W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker’s yeast will grow on W-L Nutrient Medium. W-L Nutrient Medium is also referred to as “Wallerstein Laboratory Medium”.For more information visit https://www.neogen.com/solutions/Microbiology/wl-nutrient-agar
NEOGEN Casein Digest, 500 g
Casein Digest is an enzymatic digest of casein for use in preparing microbiological culture media in a laboratory setting. Casein Digest is not intended for use in the diagnosis of disease or other conditions in humans.Casein is the main protein of milk and a rich source of amino nitrogen. Casein Digest was developed for use in molecular genetic media formulations, including NZCYM Broth, NZYM Broth, and NZM Broth. The enriched formulations of NZ media are used to cultivate recombinant strains of E. coli and propagating theta bacteriophages.For more information visit https://www.neogen.com/solutions/Microbiology/casein-digest
NEOGEN Agar, Technical, 500 g
Agar, Technical is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Technical is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to US Pharmacopeia (USP).Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperatures for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-technical
NEOGEN Reinforced Clostridial Medium, 10 kg
Reinforced Clostridial Medium is used for the cultivation of anaerobes from food. This medium conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Reinforced Clostridial Medium (RCM) is recommended by the Harmonized Pharmacopoeia for the selective enrichment of Clostridia from non-sterile samples and conforms to USP/EP/JP performance specifications. Peptone, beef and yeast extract provide a source of nitrogen, essential vitamins and amino acids. Starch aids the detoxification of harmful metabolites and glucose is a fermentable carbohydrate. Sodium chloride provides osmotic balance and sodium acetate acts as a buffer. L-Cysteine hydrochloride acts as a reducing agent to create an anaerobic environment and maintain a low Eh.For more information visit https://www.neogen.com/solutions/Microbiology/reinforced-clostridial-medium
NEOGEN Kanamycin Aesculin Azide Agar, 5 kg
Kanamycin Aesculin Azide Agar is a selective isolation and enumeration medium for enterococci (Lancefield group D streptococci) in food, and is not intended for use in the diagnosis of disease or other conditions in humans.Sodium azide and kanamycin provide the selective inhibition required whilst aesculin and iron salts form an indicator system for the presumptive identification of enterococci. Incubation at 42°C will increase the medium’s selectivity.Formula:Tryptone - 20.0 g/LYeast Extract - 5.0 g/LSodium Chloride - 5.0 g/LSodium Citrate - 1.0 g/LAesculin - 1.0 g/LFerric Ammonium Citrate - 0.5 g/LSodium Azide - 0.15 g/LKanamycin Sulphate - 0.02 g/LAgar- 10.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/kanamycin-aesculin-azide-agar
NEOGEN Bile Aesculin Agar, 10 kg
Bile Esculin Agar is used for the selective isolation and differentiation of group D streptococci from foods and is not intended for use in the diagnosis of disease or other conditions in humans.Bile Esculin Agar is based on the formulation described by Swan and further evaluated by Facklam and Moody. Rochaix first noted the value of esculin hydrolysis in the identification of enterococcci. Meyer and Schonfeld added bile to the esculin medium and demonstrated 61 of 62 enterococci strains were able to grow and hydrolyze esculin, while other streptococci could not.Molecular taxonomic studies of the genus Streptococcus have placed enterococci, previously described as group D streptococci, in the genus Enterococcus. The ability to hydrolyze esculin in the presence of bile is a characteristic of enterococci and group D streptococci.For more information visit https://www.neogen.com/solutions/Microbiology/bile-esculin-agar
NEOGEN XLT4 Agar, 10 kg
XLT4 Agar is used with Tergitol 4 for the isolation of non-typhi Salmonella spp and is not intended for use in the diagnosis of disease or other conditions in humans. XLT4 Agar is a selective differential isolation medium for the specific detection of Salmonella spp. from environmental and food samples. Due to its highly selective nature, XLT4 Agar is particularly effective when used with samples where overgrowth of contaminating flora is expected, for example, fecally contaminated agricultural samples. Developed to perform as per Miller & Tate in 1990, this medium was found to improve the recovery of non-typhi Salmonella from chicken and farm environmental samples. Dusch and Altwegg further established the application of XLT4 Agar to salmonellae detection in clinical samples, with the notable exceptions of Salmonella typhi and Salmonella paratyphi.For more information visit https://www.neogen.com/solutions/Microbiology/xlt4-agar
NEOGEN Water Plate Count Agar, 500 g
Water Plate Count Agar is a non-selective medium which conforms to ISO 6222:1999, and is not intended for use in the diagnosis of disease or other conditions in humans.Water Plate Count Agar allows the enumeration of culturable microorganisms, which can be used for the assessment and surveillance of water quality. Colony counts are useful for assessment of ground water integrity and the efficiency of water treatment processes. They also give an indication of the cleanliness and integrity of the distribution system. They can be used to assess the suitability of a water supply for the preparation of food and drink, thus avoiding contamination of the product with spoilage organisms. The main value of colony counts lies in the detection of changes in water supply quality from those expected, based on frequent long-term monitoring.For more information visit https://www.neogen.com/solutions/Microbiology/water-plate-count-agar
NEOGEN Bile Aesculin Agar, 500 g
Bile Esculin Agar is used for the selective isolation and differentiation of group D streptococci from foods and is not intended for use in the diagnosis of disease or other conditions in humans.Bile Esculin Agar is based on the formulation described by Swan and further evaluated by Facklam and Moody. Rochaix first noted the value of esculin hydrolysis in the identification of enterococcci. Meyer and Schonfeld added bile to the esculin medium and demonstrated 61 of 62 enterococci strains were able to grow and hydrolyze esculin, while other streptococci could not.Molecular taxonomic studies of the genus Streptococcus have placed enterococci, previously described as group D streptococci, in the genus Enterococcus. The ability to hydrolyze esculin in the presence of bile is a characteristic of enterococci and group D streptococci.For more information visit https://www.neogen.com/solutions/Microbiology/bile-esculin-agar
NEOGEN PPLO Broth Base without Crystal Violet, 500 g
This item has a minimum order quantity of 2 per supplier requirementsPPLO Broth Base without Crystal Violet is used with enrichments for the isolation and cultivation of Mycoplasma spp. in a laboratory setting. PPLO Broth without Crystal Violet is not intended for the use in the diagnosis of disease or other conditions in humans. PPLO Broth Base without Crystal Violet is prepared according to the formula described by Morton and Lecce. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as “pleuropneumonia-like organism” or PPLO. Mycoplasmas belong to the class of Mollicutes “soft skin” They are pleomorphic, varying in size from 0.2 to 0.3 μm.For more information visit https://www.neogen.com/solutions/Microbiology/pplo-broth-base-without-crystal-violet
NEOGEN Campy Blood-Free Selective Medium (Modified CCDA) (EMEA), 500 g
Campylobacter Blood-Free Selective Medium (Modified Charcoal Cefoperazone Deoycholate Agar or mCCDA) is used for the cultivation of campylobacters, and is not intended for use in the diagnosis of disease or other conditions in humans.A blood free medium which will support the growth of most enteric campylobacters. The selective supplement X112 (X212) makes the medium selective for C. jejuni. and C. coli when incubated at 37°C. With this product incubation at 42°C is no longer necessary and higher recovery rates have been reported at 37°C than at 42°C. The supplement X112 (X212) consists of cefoperazone and amphotericin and is superior to the selective combination of Skirrow, Butzler and Blazer-Wang all of which contain antibiotics shown to be inhibitors to C. coli. The colonial morphologies of Campylobacter spp. on this medium are distinctive.For more information visit https://www.neogen.com/solutions/Microbiology/campy-blood-free-selective-medium-modified-ccda-emea
NEOGEN Campy Blood-Free Selective Medium (Modified CCDA) (EMEA), 5 kg
Campylobacter Blood-Free Selective Medium (Modified Charcoal Cefoperazone Deoycholate Agar or mCCDA) is used for the cultivation of campylobacters, and is not intended for use in the diagnosis of disease or other conditions in humans.A blood free medium which will support the growth of most enteric campylobacters. The selective supplement X112 (X212) makes the medium selective for C. jejuni. and C. coli when incubated at 37°C. With this product incubation at 42°C is no longer necessary and higher recovery rates have been reported at 37°C than at 42°C. The supplement X112 (X212) consists of cefoperazone and amphotericin and is superior to the selective combination of Skirrow, Butzler and Blazer-Wang all of which contain antibiotics shown to be inhibitors to C. coli. The colonial morphologies of Campylobacter spp. on this medium are distinctive.For more information visit https://www.neogen.com/solutions/Microbiology/campy-blood-free-selective-medium-modified-ccda-emea
NEOGEN PPLO Broth Base without Crystal Violet, 10 kg
PPLO Broth Base without Crystal Violet is used with enrichments for the isolation and cultivation of Mycoplasma spp. in a laboratory setting. PPLO Broth without Crystal Violet is not intended for the use in the diagnosis of disease or other conditions in humans. PPLO Broth Base without Crystal Violet is prepared according to the formula described by Morton and Lecce. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as “pleuropneumonia-like organism” or PPLO. Mycoplasmas belong to the class of Mollicutes “soft skin”, which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 μm.For more information visit https://www.neogen.com/solutions/Microbiology/pplo-broth-base-without-crystal-violet
NEOGEN Letheen Broth with Tween, Modified, 10 kg
Letheen Broth with Tween, Modified is used for the recovery of microorganisms from cosmetics in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical as disinfectant residues can result in a false negative (no-growth) test result.Letheen Broth with Tween, Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-with-tween-modified
NEOGEN Campylobacter Enrichment Broth (Bolton Broth), 500 g
Campylobacter Enrichment Broth (Bolton Broth) is used with antimicrobics for the selective enrichment of Campylobacter spp. Campylobacter Enrichment Broth (Bolton Broth) is not intended for use in the diagnosis of disease or other conditions in humans. Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen. Campylobacter Enrichment Broth (Bolton) is recommended in food testing. Blood-Free Campylobacter Enrichment Broth, Bolton Broth (2X Concentration) is described by the USDA.For more information visit https://www.neogen.com/solutions/Microbiology/campylobacter-enrichment-broth-bolton