Additional Selective and Differential Cell Culture Media
NEOGEN Soy Peptone, 500 g
Soy Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans. Prepared using the enzyme papain to digest soyabean meal, this peptone is a rich source of nutrients with a high carbohydrate content. Most organisms will grow rapidly in this peptone but some bacteria will produce high levels of acid leading to auto-sterilization unless an adequate buffering system is incorporated.For more information visit https://www.neogen.com/solutions/Microbiology/soy-peptone
NEOGEN EC Medium, Modified with Novobiocin, 500 g
EC Medium, Modified with Novobiocin is used for the selective enrichment of Escherichia coli O157:H7 in a laboratory setting. EC Medium, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria and fecal streptococci were inhibited by the bile salts.EC Medium, Modified with the addition of Novobiocin was first described by Okrend and Rose. Okrend and Rose modified EC Medium by reducing the Bile Salts Mixture concentration to 1.12% and adding 20 mg/L of sodium novobiocin.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-modified-with-novobiocin
NEOGEN Harlequin Tryptone Bile Glucuronide Agar (TBX), 5 kg
Harlequin Tryptone Bile Glucuronide Agar (TBX) is used for the enumeration of E. coli in food and animal feed and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone Bile Glucuronide Agar is recommended by ISO 16649-1:2001, ISO 16649-2:2001 and ISO 16649-3:2015 for enumeration of β-glucuronidase positive Escherichia coli in products intended for human consumption or for the feeding of animals. The medium contains tryptone as a source of nitrogen and amino acids, bile salts act as a selective against Gram-positive organisms and agar is the gelling agent. 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-glucuronide) is a chromogen for the detection of the β-D-glucuronidase enzyme which is present in most E. coli* but not other coliforms.*96-97% of E.coli are β-D-glucuronidase positive. A notable exception is E. coli O157:H7 For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-tryptone-bile-glucuronide-agar-tbx
NEOGEN Harlequin Cronobacter Isolation Agar, 500 g
Chromogenic Cronobacter Isolation Agar (CCI) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Tryptic digest of casein and yeast extract provide a source of nitrogen and essential vitamins. The high content of sodium chloride and the presence of sodium desoxycholate produce a selective and specific medium.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-cronobacter-isolation-agar
NEOGEN Sodium Chloride (Bacteriological), 5 kg
Sodium Chloride is a salt often used when preparing microbiological culture media in a laboratory setting. Sodium Chloride is not intended for use in the diagnosis of disease or other conditions in humans. When added to culture media Sodium Chloride allows for adjustments in osmotic pressure.For more information visit https://www.neogen.com/solutions/Microbiology/sodium-chloride-bacteriological
NEOGEN Lowenstein-Jensen Medium, 5 kg
Lowenstein - Jensen Medium is used with fresh egg and glycerol for the isolation and differentiation of Mycobacterium spp. in a laboratory setting. Lowenstein - Jensen Medium is not intended for use in the diagnosis of disease or other conditions in humans.The use of egg-based media for primary isolation of mycobacteria have two significant advantages. First, egg-based media support a wide variety of mycobacteria. Second, growth of mycobacteria on egg media can be used for niacin testing. Liquefaction of Lowenstein-Jensen Medium can occur if contaminated with proteolytic organisms.Lowenstein-Jensen Medium is a modification of Lowenstein Medium, modified by Jensen. Jensen modified the medium by alternating the citrate and phosphate contents, eliminating congo red dye, and increasing malachite green concentration.For more information visit https://www.neogen.com/solutions/Microbiology/lowenstein-jensen-medium
NEOGEN m-HPC Agar, 5 kg
m-HPC Agar is used for the enumeration of heterotrophic organisms in water by the membrane filtration technique in a laboratory setting. m-HPC Agar is not intended for use in the diagnosis of disease or other conditions in humans.m-HPC Agar, also known as m-Heterotrophic Plate Count Agar, was developed by Taylor and Geldreich in 1979. Monitoring the levels of heterotrophic bacteria, those microorganisms that can grow on minimal organic nutrients, is currently performed to indicate problems with treatment methods within distribution systems. There is evidence that heterotrophic bacteria are not hazardous to healthy individuals, but may be opportunistic pathogens capable of infecting individuals with impaired immune systems.m-HPC Agar is used in standard methods for the detection of heterotrophic bacteria using the membrane filtration technique.For more information visit https://www.neogen.com/solutions/Microbiology/m-hpc-agar
NEOGEN Tomato Juice Agar, 500 g
Tomato Juice Agar is used for the cultivation of lactobacilli in a laboratory setting. Tomato Juice Agar is not intended for use in the diagnosis of disease or other conditions in humans. In 1925, Mickle and Breed reported the use of tomato juice in culture media used for cultivating lactobacilli. Kulp investigated tomato juice on bacterial development and found growth of L. acidophilus was enhanced. Tomato Juice Agar, prepared according to Kulp and White’s modification, is especially useful in cultivating L. acidophilus from foodstuffs.For more information visit https://www.neogen.com/solutions/Microbiology/tomato-juice-agar
NEOGEN Brilliant Green Agar, 5 kg
Brilliant Green Agar is used for the selective isolation of Salmonella spp. in a laboratory setting. Brilliant Green Agar is not intended for use in the diagnosis of disease or other conditions in humans.Kristensen, Lester, and Jurgens first described the use of Brilliant Green Agar as a primary plating medium for the isolation of Salmonella. The report described the medium as useful for the differentiation of “paratyphoid B” from other intestinal gram-negative bacilli. Kaufmann modified their formula and used Brilliant Green Agar in addition to Tetrathionate Broth for the isolation of Salmonella from stool specimens. The outstanding selectivity of this medium permits use of moderately heavy inocula, which should be evenly distributed over the surface. Brilliant Green Agar is used in the microbial limits test. Brilliant Green Agar supplemented with novobiocin is used in food testing.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-ncm0283
NEOGEN Half Fraser (Demi-Fraser) Broth (ISO), 5 kg
Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans. Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization. Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving.For more information visit https://www.neogen.com/solutions/Microbiology/half-fraser-broth
NEOGEN Tryptic Soy Agar (Soybean-Casein Digest Agar), 10 kg
Tryptic Soy Agar is used for the preparation and maintenance of test strains used in growth promotion tests, suitability of the counting methods and as negative controls as described in the Harmonized USP/EP/JP. It is also a support plating medium for various protocols described in the microbial enumeration test section of the Harmonized USP/EP/JP. Tryptic Soy Agar is not intended for use in the diagnosis of disease or other conditions in humans.Enzymatic digests of casein and soybean act as a source of nitrogen and amino acid and sodium chloride maintains the osmotic balance.Formula:Enzymatic Digest of Casein - 15.0 g/LEnzymatic Digest of Soybean - 5.0 g/LSodium Chloride - 5.0 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-agar
NEOGEN Sabouraud Dextrose Agar, 5 kg
Sabouraud Dextrose Agar is used for the cultivation of fungi. Conforms to Harmonized USP/EP/JP performance requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopeia for the isolation and identification of Candida albicans from non-sterile samples. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to SDA. The medium is also used for the preparation and maintenance of fungal test strains as described by the Harmonized Pharmacopeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating which will cause a drop in the gel strength.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar
NEOGEN Columbia Agar
Columbia Agar is used with or without blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Conforms to Harmonized USP/EP/JP Requirements. Columbia Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Clostridia from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Originally described as a general purpose nutritious agar base by Ellner et al. at Columbia University that can be enriched by the addition of sterile blood. The peptone mixture and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/columbia-agar
NEOGEN Orange Serum Agar, 10 kg
Orange Serum Agar is used for the cultivation of aciduric microorganisms associated with spoilage of products and is not intended for use in the diagnosis of disease or other conditions in humans.The low pH of fruit juices makes citrus fruit products susceptible to spoilage by yeasts, molds, and the bacteria Lactobacillus and Leuconostoc. In the 1950’s, Hays investigated spoilage in frozen concentrated orange juice. He found that an agar medium containing orange serum (juice) was superior to Lindegren Agar in isolating the microorganisms responsible for spoilage causing a buttermilk off-odor. Murdock, Folinazzo, and Troy found Orange Serum Agar, pH 5.4 to be a suitable medium for growing Leuconostoc, Lactobacillus, and yeasts. Orange Serum Agar is recommended for examining fruit beverages.For more information visit https://www.neogen.com/solutions/Microbiology/orange-serum-agar
NEOGEN Oxford Listeria Agar, 5 kg
Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water.For more information visit https://www.neogen.com/solutions/Microbiology/oxford-listeria-agar