Additional Selective and Differential Cell Culture Media
NEOGEN Buffered Peptone Water (BPW), 5 kg
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water
NEOGEN D/E Neutralizing Agar with Tween, 500 g
D/E Neutralizing Agar with Tween is used for the isolation of microorganisms from sanitized environmental surfaces and is not intended for use in the diagnosis of disease or other conditions in humans.D/E Neutralizing Agar with Tween was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycolate media, and Neutralizing Buffer.For more information visit https://www.neogen.com/solutions/Microbiology/de-neutralizing-agar-with-tween
NEOGEN Violet Red Bile Agar (VRBA), 10 kg
Violet Red Bile Agar is used for the enumeration of coliforms in food and dairy products in a laboratory setting. Violet Red Bile Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to American Public Health Association (APHA). The coliform group of bacteria includes aerobic and facultative anaerobic, gram-negative, non-spore forming bacilli. Coliforms ferment lactose and form acid and gas at 35°C within 48 hours. Members of Enterobacteriaceae comprise the majority of the group, but other lactose fermenting organisms may also be included. Procedures to detect, enumerate, and presumptively identify coliforms are used in testing foods and dairy products. One method for performing the presumptive test for coliforms uses Violet Red Bile Agar, (VRBA).For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-agar
NEOGEN HC Agar Base, 500 g
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.For more information visit https://www.neogen.com/solutions/Microbiology/hc-agar-base
NEOGEN Beef Extract Powder, 500 g
Beef Extract Powder is a dehydrated extract of bovine tissue for use in preparing microbiological culture media in a laboratory setting. Beef Extract Powder is not intended for use in the diagnosis of disease or other conditions in humans.Beef Extract Powder is prepared and standardized for use in microbiological culture media, where it is generally used to replace infusion of meat. Culture media containing Beef Extract Powder are recommended for use in bacteriological examination of water, milk, and other materials, where uniform composition of media is important. Beef Extract Powder is relied upon for biochemical studies, particularly fermentation reactions because of its independence from fermentable substances. Several media containing Beef Extract Powder are recommended in standard methods for multiple application.For more information visit https://www.neogen.com/solutions/Microbiology/beef-extract-powder
NEOGEN UVM Broth, 10 kg
UVM Broth is used for the selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.UVM Broth is a modification of the formula described by Donnelly and Baigent. This formula is used for the selective enrichment of Listeria spp. from food samples. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.For more information visit https://www.neogen.com/solutions/Microbiology/uvm-broth
NEOGEN Harlequin Tryptone Bile Glucuronide Agar (TBX), 500 g
Harlequin Tryptone Bile Glucuronide Agar (TBX) is used for the enumeration of E. coli in food and animal feed and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone Bile Glucuronide Agar is recommended by ISO 16649-1:2001, ISO 16649-2:2001 and ISO 16649-3:2015 for enumeration of β-glucuronidase positive Escherichia coli in products intended for human consumption or for the feeding of animals. The medium contains tryptone as a source of nitrogen and amino acids, bile salts act as a selective against Gram-positive organisms and agar is the gelling agent. 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-glucuronide) is a chromogen for the detection of the β-D-glucuronidase enzyme which is present in most E. coli* but not other coliforms.*96-97% of E.coli are β-D-glucuronidase positive. A notable exception is E. coli O157:H7 For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-tryptone-bile-glucuronide-agar-tbx
NEOGEN Mueller Hinton Agar II, 10 kg
Mueller Hinton Agar II is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS).Mueller Hinton Agar II is based on the formula recommended by Mueller and Hinton for the primary isolation of Neisseria species. Mueller and Hinton selected pea meal extract agar as a simple transparent medium containing heat stable ingredients. During their modification, starch replaced the growth-promoting properties of pea extract, acting as a “protective colloid” against toxic substances. Bauer, Kirby, Sherris and Tuck recommended Mueller Hinton Agar for performing antibiotic susceptibility tests using a single disk of high concentration.For more information visit https://www.neogen.com/solutions/Microbiology/mueller-hinton-agar-2
ILEX LIFE SCIENCES LLC ERYTHROPLUS SFM KIT 500 ML
PAN-Biotech ErythroPlus, Serum-Free Media Kit for Erythroid Cells (500 ml kit). INTRO: ErythroPlus is a serum-free, premium quality media kit for culture of erythroid cells (erythrocytes). The kit includes a lipid supplement and a supplement with iron-loaded deferiprone that can partially replace holotransferrin. The medium contains no animal-derived components and no phenol red. The basal medium is based on IMDM. APPLICATION: ErythroPlus is a medium developed for erythrocyte expansion and differentiation, containing components for optimal growth and proliferation under serum-free conditions. It is recommended to add Penicillin/Streptomycin and 2 mM L-Glutamine. For culturing erythroid cells, it is necessary to supplement with application-specific growth factors, such as EPO and Human Recombinant SCF. Addition of Albumin is not necessary. BICARBONATE: With 3.024 g/L NaHCO3. GLUTAMINE: Without L-glutamine. STERILE: Yes. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC MCDB 131 P04-80057 500 ML
PAN-Biotech MCDB 131 Cell Culture Medium, w/o: L-Glutamine, w: 1.176 g/L NaHCO3 (500 ml). BICARBONATE: With 1.176 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany). DESCRIPTION: MCDB 131 was developed for cultivation of human microvascular endothelial cells (HMVEC) under reduced serum content. For this purpose it is often supplemented with dialyzed serum, EGF, and hydrocortisone. MCDB 131 is suitable for other cells, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. MCDB 131 has been used for definitive endoderm differentiation of hPSCs and as a basal medium for MSCs differentiation. MCDB 131 is formulated with several unique components, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins.
ILEX LIFE SCIENCES LLC IMDM P04-20250 500 ML
PAN-Biotech IMDM Cell Culture Medium, w/o: L-Glutamine, w: 3.024 g/L NaHCO3 (500 ml). BICARBONATE: With 3.024 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Iscove's Modified Dulbecco's Medium (IMDM) is a modification of Dulbecco's Modified Eagle Medium (DMEM) that includes selenium, additional amino acids and vitamins, and sodium pyruvate. Furthermore, in IMDM, ferric nitrate (iron) is replaced with potassium nitrate. IMDM is well suited for rapidly proliferating cultures of high cell density. When supplemented with albumin, transferrin, and soy lipids it can be excellently applied for culturing lymphocytes, marrow cells, or hybridoma cells.
ILEX LIFE SCIENCES LLC MCDB 131 P04-80057 6 X 500 ML
PAN-Biotech MCDB 131 Cell Culture Medium, w/o: L-Glutamine, w: 1.176 g/L NaHCO3 (6 x 500 ml). BICARBONATE: With 1.176 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany). DESCRIPTION: MCDB 131 was developed for cultivation of human microvascular endothelial cells (HMVEC) under reduced serum content. For this purpose it is often supplemented with dialyzed serum, EGF, and hydrocortisone. MCDB 131 is suitable for other cells, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. MCDB 131 has been used for definitive endoderm differentiation of hPSCs and as a basal medium for MSCs differentiation. MCDB 131 is formulated with several unique components, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins.
ILEX LIFE SCIENCES LLC IMDM P04-20450 500 ML
PAN-Biotech IMDM Cell Culture Medium, w: stable Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 (500 ml). BICARBONATE: With 3.024 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: With Stable Glutamine. PHENOL RED: With Phenol Red. HEPES: With 25 mM HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Iscove's Modified Dulbecco's Medium (IMDM) is a modification of Dulbecco's Modified Eagle Medium (DMEM) that includes selenium, additional amino acids and vitamins, and sodium pyruvate. Furthermore, in IMDM, ferric nitrate (iron) is replaced with potassium nitrate. IMDM is well suited for rapidly proliferating cultures of high cell density. When supplemented with albumin, transferrin, and soy lipids it can be excellently applied for culturing lymphocytes, marrow cells, or hybridoma cells.
ILEX LIFE SCIENCES LLC IMDM P04-20250 6 X 500 ML
PAN-Biotech IMDM Cell Culture Medium, w/o: L-Glutamine, w: 3.024 g/L NaHCO3 (6 x 500 ml). BICARBONATE: With 3.024 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Iscove's Modified Dulbecco's Medium (IMDM) is a modification of Dulbecco's Modified Eagle Medium (DMEM) that includes selenium, additional amino acids and vitamins, and sodium pyruvate. Furthermore, in IMDM, ferric nitrate (iron) is replaced with potassium nitrate. IMDM is well suited for rapidly proliferating cultures of high cell density. When supplemented with albumin, transferrin, and soy lipids it can be excellently applied for culturing lymphocytes, marrow cells, or hybridoma cells.
NEOGEN Agar No. 2 Bacteriological, 500 g
Agar No. 2 Bacteriological is a clear bacteriological agar with high gel strength compatible with all culture media. Agar No. 2 Bacteriological is not intended for use in the diagnosis of disease or other conditions in humans.A bacteriological agar which gives a firm gel at working concentrations of 1.0 to 1.5% which is reasonably clear. This agar is recommended for all culture media except sensitivity testing media and those where absolute clarity is advantageous.For more information visit https://www.neogen.com/solutions/Microbiology/agar-number-2-bacteriological