Additional Selective and Differential Cell Culture Media
STEMCELL Technologies Conditional Reprogramming (CR) Medium, Size: 250 mL
Conditional Reprogramming (CR) Medium is optimized to use with 3T3-J2 Irradiated Feeder Cells (Catalog #100-0353) to expand epithelial cells without genetic modification. Mature epithelial cells have been shown to restore tissue-specific progenitor phenotype that enables proliferation in the presence of CR Medium (Liu X et al. 2012; Liu X et al. 2017). This conditional proliferative capability is deactivated once the medium is removed from the cells (Liu X et al. 2012; Liu X et al. 2017). CR Medium is suitable to propagate primary epithelial cells from healthy or tumor tissues (airway, retina, prostate, breast, intestine, pancreas, liver biliary duct, etc.; Liu X et al. 2012; Liu X et al. 2017; Suprynowicz FA et al.). This product is free of antibiotics and intended for Research Use Only. CR Medium must be supplemented with cholera toxin prior to use.
NEOGEN Terrific Broth, Modified, 500 g
Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans. Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli and is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.For more information visit https://www.neogen.com/solutions/Microbiology/terrific-broth-modified
NEOGEN Wort Agar, 500 g
Wort Agar is for the enumeration of yeasts and molds in butter, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium can be modified to enable it to isolate osmophilic yeasts from soft drinks and sugar products by the addition of high concentrations of sucrose and glucose.For more information visit https://www.neogen.com/solutions/Microbiology/wort-agar
NEOGEN Columbia Agar
Columbia Agar is used with or without blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Conforms to Harmonized USP/EP/JP Requirements. Columbia Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Clostridia from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Originally described as a general purpose nutritious agar base by Ellner et al. at Columbia University that can be enriched by the addition of sterile blood. The peptone mixture and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/columbia-agar
NEOGEN Buffered Sodium Chloride - Peptone Broth pH 7.0, 500 g
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans.Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-sodium-chloride-peptone-broth-ph-7
NEOGEN Acuferm Neopeptone, 500 g
For more information visit https://www.neogen.com/solutions/Microbiology/acuferm-neopeptone
NEOGEN Baird Parker Agar Base, 5 kg
Baird Parker Agar is used for the cultivation of Staphylococcus aureus from foodstuffs according to ISO 6888, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally introduced in 1962, this medium was developed by Baird-Parker to overcome the problems of recovering damaged Staphylococcus aureus from foodstuffs. This version of the medium is formulated according to ISO 6888-1:1999+A1:2003 and is in compliance with ISO 6888-2:2003+A1:2003 and ISO 6888-3:2003.Baird-Parker medium is highly selective by nature, due to the presence of potassium tellurite and lithium chloride. Tellurite inhibits most coliforms and is also reduced to telluride by S. aureus, giving the typical black colonies. Glycine and sodium pyruvate are both used as growth factors by staphylococci while the pyruvate also neutralizes any toxic peroxides that may be formed.For more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar-base
NEOGEN Enterobacteria Enrichment (EE) Broth Mossel, 500 g
Enterobacteria Enrichment (EE) Broth Mossel is used for the cultivation and enrichment of Enterobacteriaceae in food. Enterobacteria Enrichment (EE) Broth Mossel, conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. Enterobacteria Enrichment Broth Mossel was developed by Mossel, Visser, and Cornelissen to facilitate the growth of Enterobacteriaceae. This medium contains dextrose to enhance the growth of E. coli and Salmonella spp., particularly in food samples. Nuisance organisms are suppressed by the addition of Ox Bile and Brilliant Green. Enterobacteria Enrichment Broth Mossel is used as an enrichment broth, providing a rich environment for the recovery of damaged or injured cells.For more information visit https://www.neogen.com/solutions/Microbiology/enterobacteria-enrichment-broth-mossel
NEOGEN Casman Medium Base, 10 kg
Casman Medium Base is used with blood for the isolation of Haemophilus influenzae, Neisseria gonorrhoeae and other fastidious microorganisms in a laboratory setting. Casman Medium Base is not intended for use in the diagnosis of disease or other conditions in humans.In 1947, Casman described an infusion-free medium enriched with 5% blood for fastidious microorganisms incubated anaerobically. This medium replaced formulas containing fresh meat infusion and heated blood. Casman adjusted the formula after experiments revealed nicotinamide disrupted the action of a blood enzyme that inactivates V factor (NAD). The concentration of nicotinamide was lowered to support growth of Neisseria spp.For more information visit https://www.neogen.com/solutions/Microbiology/casman-medium-base
Bioworld Gamborg B5 Medium, 50 L
Gamborg B5 MediumContains Micronutrients and Macronutrients as described by Gamborg, et al. (1968)Sucrose, Cytokines, Vitamins, and Auxins should be added as needed Provided as 1 unit to make 25L or 50L of mediumWorking Concentration: 3.1 g/LpH: 3.5–4.5Soluble in waterThis product is sold for laboratory research use only
NEOGEN Skim Milk Powder, 500 g
This item has a minimum order quantity of 5 per supplier requirements
Skim Milk Powder is used when preparing microbiology culture media in a laboratory setting. Milk Powder is not intended for use in the diagnosis of disease or other conditions in humans. A bacteriological grade of thermophile free spray dried skim milk. Used in Milk Plate Count Agar NCM0119 and in media for diagnostic tests involving the digestion or coagulation of casein and the fermentation of lactose. Recommended working concentration 10%.For more information visit https://www.neogen.com/solutions/Microbiology/skim-milk-powder
NEOGEN DRBC Agar (ISO), 5 kg
Dichloran Rose Bengal Chloramphenicol Agar is for the enumeration of yeasts and is not intended for use in the diagnosis of disease or other conditions in humans.Developed with reference to ISO 21527-1:2008 for the enumeration of yeasts and molds in food and animal products, this medium is tested to the performance requirements of the standard. Used for the enumeration of viable yeasts and molds in products with a water activity of greater than 0.95 such as eggs, meat, some dairy products, fresh pastes, fruit and vegetables, DRBC Agar is designed to suppress the colonial growth of ‘spreader’ molds and in doing so allow easier performance of the colony count technique on yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-iso
NEOGEN Violet Red Bile Glucose Agar (VRBGA), 10 kg
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods. Violet Red Bile Glucose Agar conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of bile-tolerant Gram-negative bacteria from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Yeast extract provides vitamins and gelatin serves as source of carbon and nitrogen. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. The formulation is a modification of Violet Red Bile Agar by Mossel which substitutes lactose for glucose.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar
NEOGEN Presence Absence Broth, 500 g
Presence Absence Broth is used for the detection of coliform bacteria in water treatment plants or distribution systems using the presence-absence coliform test in a laboratory setting. Presence Absence Broth is not intended for use in the diagnosis of disease or other conditions in humans. The Presence Absence (PA) test is a presumptive detection for coliforms in water. The test is a simple modification of the multiple-tube procedure. One 100 mL test sample is inoculated into a single culture bottle to obtain qualitative information on the presence or absence of coliforms, through the presence or absence of lactose fermentation. This test is based on the principle that coliforms and other pollution indicator organisms should not be present in a 100 mL water sample.For more information visit https://www.neogen.com/solutions/Microbiology/presence-absense-broth
NEOGEN Tryptic Soy Broth, Modified with 8 mg Novobiocin & Acid Digest Casein, 500 g
Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods. Conforms to USDA Formulation with Novobiocin supplementation in a laboratory setting. Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth-modified-with-8-mg-novobiocin-and-acid-digest-casein