OptiCulture™ Serum-Free Medium-R (4E3-500-R) is a fully defined, nutrient-rich medium with normal glucose levels that supports healthy growth and functional phenotypes across diverse cell types. The kit includes 500 mL basal medium, OptiBoom-R (recombinant human growth factors), additional supplements (ITS, Anti-Oxidants, Hydrocortisone), and 10 mL Attachment Factor for coating flasks. It‘s medium and supplements are both xeno-free. 4E3-500-R is certified for experimental use, phenol-red free, and contains no added hormones or antibiotics. Ideal for barrier models, organoids, co-cultures, disease modeling, and drug screening.