BMEM Protein-Free Cardiomyocyte Differentiation Kit is a ready-to-use, highly optimized, chemically defined, and animal origin-free (AOF) system for the robust differentiation of human iPSCs into contracting cardiomyocytes.•Novel Protein-Free Formulation: A unique formulation optimized with no proteins to maximize consistency.•High Efficiency: Typically produces contracting cardiomyocytes in 8-9 days with ~85-90% TNNT2+ purity.•High Yield: Delivers cell yields of >1x10⁵ cells/cm².•Long-Term Culture: Resulting cardiomyocytes can be maintained for >45 days using BMEM Cardiomyocyte Maintenance Medium.•System Compatibility: Designed for use with BMEM Human Primed Pluripotent medium and compatible with standard matrices (e.g., Matrigel, Geltrex™, Vitronectin).•Quality Control: Each lot is performance tested on hiPSCs and confirmed sterile.