Cell Lines and Cell Cultures
Genscript Corporation CHO-K1/PD-L2 Stable Cell Line
Programmed cell death 1 ligand 2 (PD-L2) is a protein that in humans is encoded by the PDCD1LG2 gene PDCD1LG2 has also been designated as CD273 (cluster of differentiation 273) Inhibitory molecules of the B7/CD28 family play a key role in the induction of immune tolerance in the tumor microenvironment The programmed death-1 receptor (PD-1) with its ligands PD-L1 and PD-L2 constitutes an important member of these inhibitory pathways PD-L2 expression was initially thought to be restricted to antigen-presenting cells such as macrophages and dendritic cells (DCs) PD-L2 expression can be induced on a wide variety of other immune cells and nonimmune cells depending on microenvironmental stimuli
Genscript Corporation CHO-K1/CD80 Stable Cell Line
Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival It is the ligand for two different proteins on the T cell surface CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation) CD80 works in tandem with CD86 to prime T cells
Genscript Corporation CHO-K1/NK2 Stable Cell Line
Tachykinins are peptides sharing a common C-terminal amino acid sequence Phe-X-Gly-Leu-Met-NH sub 2 /sub This neuropeptide family is composed of substance P neurokinin A and neurokinin B which are widely distributed in mammalian central and peripheral nervous systems These three molecules serve as both neurotransmitters and neuromodulators Their actions are mediated by binding with three distinct receptors namely NK1 NK2 and NK3 In particular NK2 is expressed in gastrointestinal tract Activation of NK2 is chiefly responsible for the regulation of intestinal motor functions (both excitatory and inhibitory) secretions inflammation and visceral sensitivity Antagonists of NK2 may be useful in the treatment of irritable bowel syndrome
Genscript Corporation CHO-K1/G15 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human G protein subunit alpha 15 (G 15) in the cytoplasm This protein enables G protein-coupled receptor binding activity and acts upstream of or within with a positive effect on calcium-mediated signaling
Genscript Corporation CHO-K1/cyno PD-L2 Stable Cell
Programmed cell death 1 ligand 2 (PD-L2) is a protein that is encoded by the PDCD1LG2 gene in humans PDCD1LG2 has also been designated as CD273 (cluster of differentiation 273) Inhibitory molecules of the B7/CD28 family play a key role in the induction of immune tolerance in the tumor microenvironment The programmed death-1 receptor (PD-1) with its ligands PD-L1 and PD-L2 constitutes an important member of these inhibitory pathways PD-L2 expression was initially thought to be restricted to antigen-presenting cells such as macrophages and dendritic cells (DCs) However PD-L2 expression can be induced on a wide variety of other immune cells and nonimmune cells depending on micro environmental stimuli
Genscript Corporation CHO-K1/mouse CTLA4 Stable Cell
Recombinant CHO-K1 cells stably overexpress Mus musculus cytotoxic T-lymphocyte-associated protein 4 (CTLA4) on the surface The surface expression of mouse CTLA4 is validated by FACS analysis This cell line is designed for cell-based binding for screening antibodies binding with mouse CTLA4 and for evaluating target binding affinity
Genscript Corporation CHO-K1/CysLT1/G15 Stable Cell
CysLT1 (Cysteinyl leukotriene receptor 1) is previous named as LTD4 receptor (leukotriene D4 receptor) It is a receptor for cysteinyl leukotrienes and has highest affinity to leukotriene D4 (LTD4) The receptor mediates contraction and proliferation of smooth muscle edema eosinophil migration and damage to the mucus layer in the lung caused by LTD4 A CysLT1 selective antagonist montelukast is used clinically in the treatment of asthma This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system The rank order of affinities for the leukotrienes is LTD4 LTE4 LTC4 LTB4
Genscript Corporation HEK293/GCGR/G15 Stable Cell L
Recombinant HEK293 cells stably overexpress human glucagon receptor (GCGR) on the surface and contain high levels of G protein G s to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/Cyno VISTA Stable Cell
V-domain Ig suppressor of T cell activation (VISTA) is a potent negative regulator of T-cell function that is expressed on hematopoietic cells VISTA levels are heightened within the tumor microenvironment in which its blockade can enhance antitumor immune responses in mice In humans blockade of the related programmed cell death 1 (PD-1) pathway has shown great potential in clinical immunotherapy trials
Genscript Corporation CHO-K1/PAC1/G15 Stable Cell L
Recombinant CHO-K1 cells stably overexpress human pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1) on the surface and contain high levels of G protein G q and G s to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/VPAC1/G15 Stable Cell
The widespread neuropeptide vasoactive intestinal peptide (VIP) has two receptors VPAC1 and VPAC2 The vasoactive intestinal polypeptide receptor VPAC1 is G sub s /sub -coupled GPCRs expressed in the lung prostate peripheral blood leukocytes liver brain small intestine colon heart spleen placenta kidney thymus and testis
Genscript Corporation CHO-K1/G15/AMY2 Stable Cell L
Receptor activity-modifying proteins (RAMPs) are a class of protein which interact with and modulate the activities of several Class B G Protein-Coupled Receptors including the receptors for secretin calcitonin (CT) glucagon and vasoactive intestinal peptide (VIP) There are three distinct types of RAMPs designated RAMP1 RAMP2 and RAMP3 each encoded by a separate gene Currently the function of RAMPs is divided into 2 class activities Association of RAMPs with either the CT or CALCR proteins forms 6 different receptors from the calcitonin receptor family When associated with the Calcitonin receptor (CTR) or Calcitonin receptor-like (CALCRL) RAMPs can change the selectivity of the receptor for a specific hormone In the cases of the other receptors mentioned however there is no evidence that they can do this but instead function to regulate trafficking of receptors from the ER / golgi to the membrane GenScript s cloned human AMY2 (RAMP2 CALCR) expressing cell line is gen
Genscript Corporation CHO-K1/H3/G15 Stable Cell Lin
The histamine receptor H3 is a G sub i /sub -coupled GPCR expressed in the thalamus caudate nucleus putamen cerebellum amygdala substantia nigra hippocampus hypothalamus and cerebral cortex Activation of H3 receptor in human nasal mucosa inhibits sympathetic vasoconstriction Expression of human H3 receptor splice variants in CHO cells shows that a deletion in the 2nd transmembrane domain alters ligand binding and deletions in the 3rd intracellular loop result in signal transduction being abolished
Genscript Corporation CD47 Human
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRP ) CD-47 acts as a don t eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers
Genscript Corporation CHO-K1/CT/G15 Stable Cell Lin
Recombinant CHO-K1 cells stably overexpress human calcitonin receptor (CT) on the surface and contain high levels of G protein G q and G s to couple with the receptor in downstream signaling pathways