Quantitative synthetic Human metapneumovirus hMPV RNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. This preparation includes the N Gene (mRNA-Nucleoprotein), P Gene (mRNA-Phosphoprotein), M Gene (mRNA-Matrix Protein), F Gene (mRNA-Fusion Glycoprotein), and L Gene (mRNA-RNA Dependent RNA Polymerase).